Effect of growth conditions of Lactobacillus gasseri OLL2809 on the immunostimulatory activity for production of interleukin-12 (p70) by murine splenocytes

Sashihara, Toshihiro; Sueki, Natsuko; Furuichi, K.; Ikegami, Shuji

doi:10.1016/j.ijfoodmicro.2007.09.003

Cited by 40 publications

(33 citation statements)

References 28 publications

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“…Out of many strains in our LAB library, we had previously selected the optimal OLL2712 strain, which has high IL-10-inducing activity (15). On the other hand, the health-promoting properties of LAB may change in response to their culture conditions, even when the strain remains the same (7)(8)(9)(10)(11). With respect to OLL2712, we expected that the anti-inflammatory activity would be changed by modification of the culture conditions.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the screening of strains, enhancing the abilities of selected strains improves the healthpromoting abilities of LAB. Moreover, the immunomodulatory properties of a given LAB strain are reportedly sensitive to changing culture conditions (7)(8)(9)(10)(11). It was also reported that the amount and structure of teichoic acids, which are principal immunomodulatory components in LAB, depended on culture conditions (12)(13)(14).…”

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confidence: 99%

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Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice

Toshimitsu

Ozaki

Mochizuki

et al. 2017

Appl Environ Microbiol

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Studies on the health-promoting effects of lactic acid bacteria (LAB) are numerous, but few provide examples of the relationship between LAB function and culture conditions. We verified the effect of differences in culture conditions on Lactobacillus plantarum OLL2712 functionality; this strain exhibits anti-inflammatory activity and preventive effects against metabolic disorders. We measured interleukin-10 (IL-10) and IL-12 production in murine immune cells treated with OLL2712 cells prepared under various culture conditions. The results showed that the IL-10-inducing activities of OLL2712 cells on murine immune cells differed dramatically between OLL2712 groups at different culture phases and using different culture medium components, temperatures, and neutralizing pHs. In particular, exponential-phase cells had much more IL-10-inducing activity than stationary-phase cells. We confirmed that the Toll-like receptor 2 (TLR2) stimulation activity of OLL2712 cells depended on culture conditions in conjunction with IL-10-inducing activity. We also demonstrated functional differences by culture phases in vivo; OLL2712 cells at exponential phase had more anti-inflammatory activity and anti-metabolic-disorder effects on obese and diabetic mice than those by their stationary-phase counterparts. These results suggest that culture conditions affect the functionality of antiinflammatory LAB.IMPORTANCE While previous studies demonstrated that culture conditions affected the immunomodulatory properties of lactic acid bacteria (LAB), few have comprehensively investigated the relationship between culture conditions and LAB functionality. In this study, we demonstrated several culture conditions of Lactobacillus plantarum OLL2712 for higher anti-inflammatory activity. We also showed that culture conditions concretely influenced the health-promoting functions of OLL2712 in vivo, particularly against metabolic disorders. Further, we characterized a novel mechanism by which changing LAB culture conditions affected immunomodulatory properties. Our results suggest that culture condition optimization is important for the production of LAB with anti-inflammatory activity.KEYWORDS culture phase, dendritic cell, interleukin-10, interleukin-12, Lactobacillus plantarum, macrophage, metabolic disorder, mono-oleic acid ester, proinflammatory cytokine, Toll-like receptor L actic acid bacteria (LAB) have been reported to modulate immune activities and prevent or ameliorate immune-related disorders, including inflammatory bowel diseases, allergies, and certain metabolic disorders (1-3). Certain LAB strains have been selected as the most suitable strains against each disorder (4-6). In addition to the

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice

Toshimitsu

Ozaki

Mochizuki

et al. 2017

Appl Environ Microbiol

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“…L. gasseri OLL2809 has been selected on the basis of its immunostimulatory activity from 20 of potential probiotic candidates (Sashihara et al 2006). It stimulates production of IL-12 from antigen-presenting cells such as macrophage and dendritic cells (Sashihara et al 2007;Sashihara et al 2008). The immunostimulatory effect of the strain was also observed in a clinical study with patients with Japanese cedar pollinosis (Gotoh et al 2009).…”

Section: Gene Expression Analysis Of Peritoneal Cellsmentioning

confidence: 99%

“…Lactobacillus gasseri OLL2809 is one of a probiotic lactobacillus that has been selected on the basis of its immunostimulatory activity (Sashihara et al 2006(Sashihara et al , 2007. We previously reported that the oral administration of heat-killed L. gasseri OLL2809 effectively enhances production of IL-12 in splenocytes ex vivo and shifts the Th1/Th2 balance from Th2-dominant immunity toward Th1-dominant immunity (Sashihara et al 2008;Gotoh et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus gasseri OLL2809 inhibits development of ectopic endometrial cell in peritoneal cavity via activation of NK cells in a murine endometriosis model

et al. 2011

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We have previously reported that peritoneal administration of interleukin-12 (IL-12) suppresses development of ectopic endometriotic lesions via activation of natural killer (NK) cells in a mouse endometriosis model. Lactobacillus gasseri OLL2809 is one of a probiotic lactobacillus that has been selected on an ability to stimulate production of IL-12 from murine splenocytes. In this study, we examined whether the oral administration of heatkilled L. gasseri OLL2809 suppressed development of endometriosis. Administration of L. gasseri OLL2809 for 21 consecutive days resulted in reduction of the development of ectopic endometriotic lesions in an extent similar to IL-12. Although obvious effects of L. gasseri OLL2809 on the peritoneal cytokine levels, population of peritoneal cells as well as cytotoxicity of splenic NK cells, gene expression analysis of the peritoneal cells revealed enhancement in the transcription of IL-2 and natural killer cell triggering receptor 1 genes. Therefore, it was suggested that L. gasseri OLL2809 suppressed development of endometriosis via activation of NK cells.

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“…Sashihara et al reported that the IL-12-inducing activity of heat-killed bacteria was higher than of live bacteria if cultured with immune cells under a neutral pH condition, but that IL-12-inducing activities of live and heat-killed bacteria were equal if bacteria were cultured under a low pH condition. 24) They concluded that these phenomena might be induced by the modification of peptidoglycan conformation or degradation of cell wall structure via autolysis. Some reports, including our study, have indicated that the cell wall is an important component for IL-12 production from immune cells.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Immunomodulatory Effects of Live and Heat-killed Lactobacillus pentosus S-PT84

Izumo

Ida

Maekawa

et al. 2011

JOURNAL OF HEALTH SCIENCE

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Lactobacillus pentosus S-PT84, which was isolated from Kyoto pickles, enhances splenic natural killer (NK) cell activity, increases IgA production from Peyer's patch cells and has high T-helper 1 (Th1) cytokine and type 1-interferon-inducing activity. However, all of these effects were obtained by using heat-killed S-PT84. In the present study, we investigated the immunomodulatory effect of live and heat-killed S-PT84 in BALB/c mice. Unexpectedly, live S-PT84 indicated weak activity on Th1 cytokine (interleukin-12 or interferon-γ) production from splenocytes compared to heat-killed S-PT84 in vitro. Similar tendencies were also shown in S-PT84 having different cell wall thickness. Next, we compared the immunomodulatory effect after oral ingestion of each S-PT84. The results indicated splenic NK activity was equally enhanced by live or heat-killed S-PT84 ingestion, though heat-killed S-PT84 indicated slightly higher activity in interferon-γ production and inclination toward Th1 immunity. These results suggested that both live and heat-killed S-PT84 have an equal immunomodulatory effect in BALB/c mice. It is thought that the use of heat-killed S-PT84 is advantageous from the viewpoint of ease of handling compared to live S-PT84.

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Effect of growth conditions of Lactobacillus gasseri OLL2809 on the immunostimulatory activity for production of interleukin-12 (p70) by murine splenocytes

Cited by 40 publications

References 28 publications

Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice

Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice

Lactobacillus gasseri OLL2809 inhibits development of ectopic endometrial cell in peritoneal cavity via activation of NK cells in a murine endometriosis model

Comparison of the Immunomodulatory Effects of Live and Heat-killed Lactobacillus pentosus S-PT84

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