Lactobacillus gasseri OLL2809 inhibits development of ectopic endometrial cell in peritoneal cavity via activation of NK cells in a murine endometriosis model

Abstract

We have previously reported that peritoneal administration of interleukin-12 (IL-12) suppresses development of ectopic endometriotic lesions via activation of natural killer (NK) cells in a mouse endometriosis model. Lactobacillus gasseri OLL2809 is one of a probiotic lactobacillus that has been selected on an ability to stimulate production of IL-12 from murine splenocytes. In this study, we examined whether the oral administration of heatkilled L. gasseri OLL2809 suppressed development of endometriosis. Administration of L. gasseri OLL2809 for 21 consecutive days resulted in reduction of the development of ectopic endometriotic lesions in an extent similar to IL-12. Although obvious effects of L. gasseri OLL2809 on the peritoneal cytokine levels, population of peritoneal cells as well as cytotoxicity of splenic NK cells, gene expression analysis of the peritoneal cells revealed enhancement in the transcription of IL-2 and natural killer cell triggering receptor 1 genes. Therefore, it was suggested that L. gasseri OLL2809 suppressed development of endometriosis via activation of NK cells.

Involvement of immune cells in the pathogenesis of endometriosis

“…Itoh et al conducted mouse model experiments and found that the oral administration of probiotics activates peritoneal NK cells, and reduces endometriotic lesion formation. 60 Further, an extract of the white mistletoe tree, Helixor A, also directly activates NK cell cytotoxicity. 56 By adapting a strategy for cancer immunotherapy, 61 Montenegro et al administered intraperitoneal injections of IL-2-activated NK cells and reduced lesion formation in a mouse endometriosis model.…”

“…Itoh et al . conducted mouse model experiments and found that the oral administration of probiotics activates peritoneal NK cells, and reduces endometriotic lesion formation . Further, an extract of the white mistletoe tree, Helixor A, also directly activates NK cell cytotoxicity .…”

Effects ofLactobacillus gasseriOLL2809 on the Induced Endometriosis in Rats

“…We have reported the preventive efficacy of OLL2809 on a mouse endometriosis model by activating NK cells. 5) We evaluated the effect of OLL2809 on existing endometriosis in rats. In addition, recently we reported that the estrus cycle affected the healing process in a rat implanted endometriosis model, and found that the autoimplanted endometriosis in the diestrus did not heal readily by sequential endoscopic observation.…”

“…We observed recently a suppressive effect of IL-12 on the development of ectopic endometrial lesions, and found that it was due to activation of NK cells. 5) OLL2809 is a probiotic Lactobacillus that was selected on the basis of its immune stimulatory activity.…”

“…4) OLL2809 suppressed the development of endometriosis by activation of natural killer (NK) cells in a mouse endometriosis model, 5) but the efficacy of OLL2809 has not been clarified on already existing endometriosis in basic research. On the other hand, Katuki et al 6) have reported that dienogest reduced the endometrial implant volume to the same extent as danazol did.…”