Effect of green and red light in lipid accumulation and transcriptional profile of genes implicated in lipid biosynthesis in <i>Chlamydomonas reinhardtii</i>

Gaytán-Luna, Daniel Eugenio; Ochoa-Alfaro, Ana Erika; Rocha‐Uribe, Alejandro; Pérez-Martínez, Ana S.; Alpuche-Solís, Ángel G.; Soria-Guerra, Ruth Elena

doi:10.1002/btpr.2368

Cited by 15 publications

(8 citation statements)

References 47 publications

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“…Similarly, Atta et al [48] found that the lipid content of C. vulgaris increased when the light intensity with blue LED lamps increased from 100 to 200 µmol photons m −2 s −1 at 12:12 h light: dark. Gaytán-Luna et al [58] studied the effect of green, white, and red light on the lipid content of Chlamydomonas reinhardtii. They found that the maximum lipid content was observed when cultivated at red light in comparison with cultivation at white and green light.…”

Section: Experiments In the Closed Bioreactor-effect Of Wavelength Anmentioning

confidence: 99%

Effect of Light Intensity and Quality on Growth Rate and Composition of Chlorella vulgaris

Metsoviti

Papapolymerou

Karapanagiotidis

et al. 2019

Plants

143

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In this research, the effect of solar irradiance on Chlorella vulgaris cultivated in open bioreactors under greenhouse conditions was investigated, as well as of ratio of light intensity in the 420–520 nm range to light in the 580–680 nm range (I420–520/I580–680) and of artificial irradiation provided by red and white LED lamps in a closed flat plate laboratory bioreactor on the growth rate and composition. The increase in solar irradiance led to faster growth rates (μexp) of C. vulgaris under both environmental conditions studied in the greenhouse (in June up to 0.33 d−1 and in September up to 0.29 d−1) and higher lipid content in microalgal biomass (in June up to 25.6% and in September up to 24.7%). In the experiments conducted in the closed bioreactor, as the ratio I420–520/I580–680 increased, the specific growth rate and the biomass, protein and lipid productivities increased as well. Additionally, the increase in light intensity with red and white LED lamps resulted in faster growth rates (the μexp increased up to 0.36 d−1) and higher lipid content (up to 22.2%), while the protein, fiber, ash and moisture content remained relatively constant. Overall, the trend in biomass, lipid, and protein productivities as a function of light intensity was similar in the two systems (greenhouse and bioreactor).

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Section: Experiments In the Closed Bioreactor-effect Of Wavelength Anmentioning

confidence: 99%

Effect of Light Intensity and Quality on Growth Rate and Composition of Chlorella vulgaris

Metsoviti

Papapolymerou

Karapanagiotidis

et al. 2019

Plants

143

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“…In our previous work, the expression profile of the BCX1 and ACP1 genes in C. reinhardtii cultures exposed to red (650 nm) and green (550 nm) light were down‐regulated when compared with white light (control experiment); while the acyl‐ACP thiolase ( FAT1 ) and acyl‐sn‐glycerol‐3‐phosphate acyltransferase (PLS1) genes were up‐regulated under red light with respect to green light . According to this, the gene expression profile implicated in the early steps of the fatty acid synthesis may be impacted differently according to the stress conditions.…”

Section: Discussionmentioning

confidence: 90%

“…The reaction mixture in a total volume of 10 μL contained 100 ng of total cDNA, 5 μL of SYBRGreen Master Mix reagent (Applied Biosystems, Mulgrave, Australia), and 100 nM of each primer. The oligonucleotides sequences used in this study were previously described . RT‐PCR conditions were 10 min at 95°C, followed by 40 cycles of 15 s at 95°C (denaturation), 30 s at 60°C (primer annealing), and 30 s at 60°C (primer extension).…”

Section: Methodsmentioning

confidence: 99%

pH effects on the lipid and fatty acids accumulation in Chlamydomonas reinhardtii

Ochoa-Alfaro

Gaytán-Luna

González‐Ortega

et al. 2019

Biotechnology Progress

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pH variations influence the delivery of essential nutrients and CO2 solubility, which impact algae metabolism. In this study the microalgal growth and chlorophyll, lipid, and fatty acids content; along with the expression of some genes implicated in the biosynthesis of lipids were examined in Chlamydomonas reinhardtii subjected to pH values of 7.0, 7.8, and 8.5. At pH 7.8 an increase in cell growth was observed with a significant accumulation of chlorophyll (1.75‐fold) when compared with growth at pH 7, while at pH 8.5 a sharp decrease in both parameters was observed when compared with the other pH values tested. Lipid content increased 3.0 (14.81% of dry cell weight, dcw) and 2.3 times (11.43% dcw) at pH 7.8 and 8.5, respectively, when compared with the experiment at pH 7 (4.97% dcw). The compositions of major fatty acids in the strains growing at pH 7.0, 7.8, or 8.5 were 25.7, 28.0, and 32.1% for palmitic acid; 17.3, 14.7, and 25.7% for oleic acid; and 9.8, 12.1, and 4.6% for linoleic acid; respectively. qRT‐PCR analysis showed that the transcripts of ß‐carboxyltransferase, Acyl carrier protein 1, acyl‐ACP thiolase 1, acyl‐sn‐glycerol‐3‐phosphate acyltransferase, and diacylglycerol acyl transferase isoform 3 were significantly induced at pH 7.8 when compared with the other two pH conditions. These results indicate that the induction of genes implicated in the early and final steps of lipid biosynthesis contributes to their accumulation in the stationary phase. Our research suggests that a pH of 7.8 might be ideal to maximize growth and lipid accumulation.

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“…For the production of ATP and NADPH and the synthesis of particles needed for growth, algae need light of appropriate wavelength and color temperature [45][46][47]. It would seem that since the light intensity improves the growth rate of the algae, the bioreactors should be illuminated as intensely as possible.…”

Section: Type Of Photobioreactor Modelmentioning

confidence: 99%

The Influence of Artificial Lighting Systems on the Cultivation of Algae: The Example of Chlorella vulgaris

Brzychczyk¹,

Hebda²,

Pedryc³

2020

Energies

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Microalgae are a practical source of biological compounds for biodiesel production. This study examined the influence of three different light-emitting diode (LED) systems on the biomass production of green algae Chlorella vulgaris BA0002a. The cultivation was carried out in a photobioreactor illuminated from the bottom with a single side light jacket (PBR I), in a photobioreactor illuminated from the bottom with a double side light jacket (PBR II) and in a photobioreactor illuminated only from the top (PBR III). Research has shown that the intensification of algae cell production and growth depends on the light distribution and exposure time of a single cell to radiation. In the experiment, the highest growth of algae cells was obtained in the photobioreactor with double jacket and lower light panel. The lowest cell growth was observed in the photobioreactor illuminated only from above. For cultures raised in the PBR I and PBR II photobioreactors, increased oxygen production was observed, which was directly related to the increased production of biomass, which in turn was dependent on the increased amount of radiant energy.

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Effect of green and red light in lipid accumulation and transcriptional profile of genes implicated in lipid biosynthesis in Chlamydomonas reinhardtii

Cited by 15 publications

References 47 publications

Effect of Light Intensity and Quality on Growth Rate and Composition of Chlorella vulgaris

Effect of Light Intensity and Quality on Growth Rate and Composition of Chlorella vulgaris

pH effects on the lipid and fatty acids accumulation in Chlamydomonas reinhardtii

The Influence of Artificial Lighting Systems on the Cultivation of Algae: The Example of Chlorella vulgaris

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