Effect of Grafted Amphiphilic PVP–Palmityl Polymers on the Thermotropic Phase Behavior of 1,2 Dipalmitoyl- sn-glycero-3-phosphocholine Bilayer

Savva, Michalakis; Torchilin, Vladimir P.; Huang, Leaf

doi:10.1006/jcis.1999.6344

Cited by 9 publications

(3 citation statements)

References 14 publications

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“…The interaction of macromolecules such as polymers and proteins with lipid membranes is important in areas as diverse as polymer science, , membrane biophysics, − and drug formulations . There is considerable interest in using supported lipid bilayers as models for biophysical processes relevant to protein adsorption, drug formulation, vesicle formation, and sensing. , Detailed characterization of these supported bilayers both by themselves and upon incorporation of various materials can provide insight into the structure and reactivity of the bilayer film.…”

Section: Introductionmentioning

confidence: 99%

Modification of a Supported Lipid Bilayer by Polyelectrolyte Adsorption

2004

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Addition of a weak polyelectrolyte, poly(methacrylic acid) (PMA), to a supported phospholipid bilayer made from 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) depresses the melting temperature and alters the morphology of the bilayer in the gel phase. Ellipsometry measurements show that PMA adsorption lowers the phase transition temperature by 2.4 degrees C. Atomic force microscopy (AFM) showed no visible contrast in the fluid phase (above the melting temperature) but a rich morphology in the gel phase. In the gel phase, adsorption leads to formation of significantly less mobile phospholipid islands and other defects. One consequence of this lower mobility is a decrease in the implied cooperativity number of the phase transition, N, when polymer is added. Additionally, AFM images of the gel-phase bilayer show a highly defected structure that anneals significantly more slowly than in the absence of adsorbed polymer. Tentatively, we suggest that PMA preferentially decorates island and defect edges of the DMPC bilayer.

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Section: Introductionmentioning

confidence: 99%

Modification of a Supported Lipid Bilayer by Polyelectrolyte Adsorption

2004

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“…[2][3][4][5][6] In the case of VP, few examples of living radical polymerisation experiments have been described so far, [7] and the resultant products usually had relatively broad polydispersities. Therefore, conventional radical polymerisation processes in the presence of functionalised chaintransfer agents (CTAs) still represent, in our opinion, a remarkably cheap and efficient route to PVP oligomers bearing reactive functions at one terminus.…”

Section: Introductionmentioning

confidence: 99%

“…End‐functionalised 1‐vinylpyrrolidin‐2‐one (VP) polymers of controlled molecular weight are biocompatible macromolecular compounds with a high potential as intermediates in the synthesis of new bioactive and bioeliminable supramolecular architectures. By virtue of their low toxicity and strong solubilising power,1 they have been proposed as an alternative to polyethylene glycol (PEG) in nano‐sized delivery systems for active substances, such as modified proteins, soluble drug carriers, nanoparticles, liposomes, and polymeric micelles 2–6…”

Section: Introductionmentioning

confidence: 99%

NMR Spectroscopy and MALDI‐TOF MS Characterisation of End‐Functionalised PVP Oligomers Prepared with Different Esters as Chain Transfer Agents

Ranucci

Ferruti

Annunziata

et al. 2006

Macromolecular Bioscience

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Ester-functionalised poly(1-vinylpyrrolidin-2-one) (PVP) oligomers obtained by radical polymerisation in methyl propionate, diethyl malonate and diethyl 2-methylmalonate were characterised by NMR spectroscopy, and MALDI-TOF mass spectrometry. The chain-transfer constants were determined as 5.54 x 10(-4), 1.22 x 10(-3) and 1.70 x 10(-2), respectively, by measuring the variation of the number-average molecular weight on conversion. These values were compared with those of methyl isobutyrate (1.65 x 10(-3)) and ethyl lactate (1.03 x 10(-2)), which had been previously determined. A clear dependence was found on the reactivity of the mobile hydrogen atoms alpha with the ester group. All of the macromolecules carried a single ester function. Therefore, the re-initiation step by the CTA-derived radicals overwhelmingly prevailed over initiation by the primary radicals.

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Probing molecular interactions of poly(styrene-co-maleic acid) with lipid matrix models to interpret the therapeutic potential of the co-polymer

Banerjee

Pal

Guha

2012

Biochimica et Biophysica Acta (BBA) - Biomembranes

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To understand and maximize the therapeutic potential of poly(styrene-co-maleic acid) (SMA), a synthetic, pharmacologically-active co-polymer, its effect on conformation, phase behavior and stability of lipid matrix models of cell membranes were investigated. The modes of interaction between SMA and lipid molecules were also studied. While, attenuated total reflection-Fourier-transform infrared (ATR-FTIR) and static (31)P nuclear magnetic resonance (NMR) experiments detected SMA-induced conformational changes in the headgroup region, differential scanning calorimetry (DSC) studies revealed thermotropic phase behavior changes of the membranes. (1)H NMR results indicated weak immobilization of SMA within the bilayers. Molecular interpretation of the results indicated the role of hydrogen-bond formation and hydrophobic forces between SMA and zwitterionic phospholipid bilayers. The extent of membrane fluidization and generation of isotropic phases were affected by the surface charge of the liposomes, and hence suggested the role of electrostatic interactions between SMA and charged lipid headgroups. SMA was thus found to directly affect the structural integrity of model membranes.

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Effect of Grafted Amphiphilic PVP–Palmityl Polymers on the Thermotropic Phase Behavior of 1,2 Dipalmitoyl- sn-glycero-3-phosphocholine Bilayer

Cited by 9 publications

References 14 publications

Modification of a Supported Lipid Bilayer by Polyelectrolyte Adsorption

Modification of a Supported Lipid Bilayer by Polyelectrolyte Adsorption

NMR Spectroscopy and MALDI‐TOF MS Characterisation of End‐Functionalised PVP Oligomers Prepared with Different Esters as Chain Transfer Agents

Probing molecular interactions of poly(styrene-co-maleic acid) with lipid matrix models to interpret the therapeutic potential of the co-polymer

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