Probing molecular interactions of poly(styrene-co-maleic acid) with lipid matrix models to interpret the therapeutic potential of the co-polymer

Banerjee, Subhasis; Pal, Tapan Kumar; Guha, Sujoy K.

doi:10.1016/j.bbamem.2011.12.010

Cited by 30 publications

(16 citation statements)

References 57 publications

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“…Colby and coworkers [74,75]. The two carboxyl groups have different pK a s of 6 and 10, which means SMA bears two charges on both carboxyl groups at high pH, one charge at neutral pH, and is not charged at low pH [76]. The conformation and solubility of SMA are also affected by the local pH: at high or neutral pH, the electrostatic repulsions of the carboxylates prevail and overcome the hydrophobic effect, therefore the polymer adopts a random coil conformation and shows good solubility in aqueous solution; at low pH (lower than pH 6), the carboxylates are protonated and the hydrophobicity of the styrene dominates, which results in a compact globular conformation with poor aqueous solubility [77].…”

Section: Modification and Functionalization Of Alternating Copolymersmentioning

confidence: 99%

Recent advances in alternating copolymers: The synthesis, modification, and applications of precision polymers

Huang

Turner

2017

Polymer

108

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Alternating copolymers represent a special class of copolymers, in which the two comonomers copolymerize in a regular alternating sequence along the chain. Their interesting physical and chemical properties, as well as the underlying mechanism, have attracted significant attention in both academia and industry. The electron-donor benzylidene monomers-styrene and stilbene, readily form alternating copolymers with the electron-acceptor monomersmaleic anhydride and N-substituted maleimides. The rich chemistry of the substitution groups on these monomers offers enormous combinations for the synthesis of alternating copolymers for different applications. In this paper, we aim to provide a general overview of recent publications on the specific field of these benzylidene-containing alternating copolymers, and the emphasis is placed on the synthetic progress, structure-property relationships, and the applications of these copolymers.

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Section: Modification and Functionalization Of Alternating Copolymersmentioning

confidence: 99%

Recent advances in alternating copolymers: The synthesis, modification, and applications of precision polymers

Huang

Turner

2017

Polymer

108

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“…49,50 To measure complement activation in vitro, we determined the NP-induced rise of human serum complement products C5a and sC5b-9 using the respective ELISA kits (Quidel, San Diego, CA, USA) according to the manufacturer's protocols as described previously. 45,46 Briefly, complement activation was initiated by adding the required quantity of TT1 loaded HSA-NPs to undiluted serum in Eppendorf tubes in a shaking water bath at 37°C for 30 minutes, unless stated otherwise. Reactions were terminated by quickly cooling samples on ice and adding 25 mM ethylenediaminetetraacetic acid (EDTA).…”

Section: Stability Of Np Solutionmentioning

confidence: 99%

Human serum albumin nanoparticles loaded with phthalocyanine dyes for potential use in photodynamic therapy for atherosclerotic plaques

Banerjee

Sengupta

Aljarilla

et al. 2019

PRNANO

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Diseases caused by obstruction or rupture of vulnerable plaques in the arterial walls such as cardiovascular infarction or stroke are the leading cause of death in the world. In the present work, we developed human serum albumin nanoparticles loaded by physisorption with zinc phthalocyanine, TT1, mainly used for industrial application as near-infrared photosensitizer and compared these to HSA NPs loaded with the well-known silicone phthalocyanine (Pc4). The use of NIR light allows for better tissue penetration, while the use of nanoparticles permits high local concentrations. The particles were characterized and tested for toxicity and stability as well as for their potential use as a contrast agent and NIR photosensitizer for photodynamic therapy in cardiovascular disease. We focused on the distribution of the nanoparticles in RAW264.7 macrophage cells and atherosclerotic mice. The nanoparticles had an average size of 120 nm according to dynamic light scattering, good loading capacity for zinc phthalocyanine, and satisfying stability in 50% (v/v) fetal bovine serum for 8 hours and in an aqueous environment at 4°C for 4–6 weeks. Under light irradiation we found a high production of singlet oxygen and the products showed no dark toxicity in vitro with macrophages (the target cells in vulnerable plaques), but at a low g/mL nanoparticle concentration killed efficiently the macrophages upon LED illumination. Injection of the contrast agent in atherosclerotic mice led to a visible fluorescence signal of zinc phthalocyanine in the atherosclerotic plaque at 30 minutes and in the lungs with a fast clearance of the nanoparticles. Zinc phthalocyanine loaded human serum albumin nanoparticles present an interesting candidate for the visualization and potentially photodynamic treatment of macrophages in atherosclerotic plaques.

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“…The styrene groups provide hydrophobic character to the polymer, and the carboxylate groups, hydrophilic character. Because of their pKa, the carboxylate groups are uncharged at a pH below 6 [72]. As a result, SMALPs are much more hydrophobic, and so they precipitate at acidic pHs.…”

Section: Polymer-based Strategies a Amphipolsmentioning

confidence: 99%

Artificial membranes for membrane protein purification, functionality and structure studies

Parmar

Lousa

Muench

et al. 2016

Biochemical Society Transactions

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Membrane proteins represent one of the most important targets for pharmaceutical companies. Unfortunately, technical limitations have long been a major hindrance in our understanding of the function and structure of such proteins. Recent years have seen the refinement of classical approaches and the emergence of new technologies that have resulted in a significant step forward in the field of membrane protein research. This review summarises some of the current techniques used for studying membrane proteins, with overall advantages and drawbacks for each method. Micelles and classical detergent techniquesMembrane proteins account for~30% of both prokaryotic and eukaryotic proteins [1]. Integral proteins such as transporters or ion channels, as well as peripheral membrane proteins such as G-proteins, all perform essential tasks in signal transduction, cell metabolism and transport of small molecules [2][3][4]. Integral and peripheral membrane proteins are respectively embedded in or closely associated with the phospholipid bilayer of cell membranes. Therefore, their function often relies on their precise lipid environment [5,6]. For instance, cardiolipin, which constitutes about 20% of the inner mitochondrial membrane, is essential to the function of many mitochondrial transporters such as the ADP/ATP carriers, the enzymes of the respiratory chain, and bacterial proteins [7][8][9]. This is because cardiolipin offers polar and electrostatic interactions that increase protein stability[10]; similar interactions have been observed for other lipids [11,12]. Unfortunately, classical techniques to study membrane proteins involve the use of detergents that solubilise the protein but also destabilise its interaction with membrane lipids. In many cases, membrane proteins may be stable in only a few detergents, limiting the range of conditions that can be used for crystallization trials [13]. Consequently, much time is spent testing various detergents in different ratios and concentrations in the hope of finding conditions that will mimic the essential interactions of the protein with its natural lipidic environment -and this way stabilise the protein and preserve its functional state. These problems have hindered membrane protein research for many years: both biophysical characterisation and structure solution have suffered due to difficulties of extracting proteins from membranes and keeping them stable away from their native environment. The past few years have seen the emergence of new techniques aimed at providing a membrane-like natural environment. These novel techniques include liposomes, bicelles, discs, polymer and lipids based strategies.

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Probing molecular interactions of poly(styrene-co-maleic acid) with lipid matrix models to interpret the therapeutic potential of the co-polymer

Cited by 30 publications

References 57 publications

Recent advances in alternating copolymers: The synthesis, modification, and applications of precision polymers

Recent advances in alternating copolymers: The synthesis, modification, and applications of precision polymers

Human serum albumin nanoparticles loaded with phthalocyanine dyes for potential use in photodynamic therapy for atherosclerotic plaques

Artificial membranes for membrane protein purification, functionality and structure studies

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