Effect of graded concentration of organic Zinc (Zinc glycinate) on antioxidants status and immune response in commercial broilers

Abstract

One hundred and twenty day old commercial broiler chicks were randomly allotted to 4 dietary groups, with 6 replicates of 5 birds in each and reared for 42 days under uniform management conditions to study the effect of organic zinc (zinc glycinate; Zn-gly) supplemented at lower levels (30, 20 and 10 ppm) on antioxidant status and immune response in comparison to NRC (1994) recommended levels (40 ppm) of Zn supplemented from inorganic source (ZnSO4). The dietary treatments were corn soybean meal basal diet supplemented with 40 ppm Zn from ZnSO4 (inorganic) (control) and 30, 20 and 10 ppm Zn from Zn-gly (organic). Antioxidant enzyme levels and antibody titres against New castle disease (ND) vaccine (humoral immunity) were estimated on 35th d and oxidative stress markers from liver on 42nd d. On 40th d, the cell mediated immunity (CMI) was assessed as cutaneous basophilic hypertrophy to phytohemagglutinin-P (PHA-P). The lipid peroxidation in haemolysate (P<0.05) lowered in birds with 30 ppm Zn supplemented as Zn-gly compared to 20 and 10 ppm Zn from Zn-gly and 40 ppm Zn from ZnSO4. The Glutathione peroxidase enzyme activity was higher (P<0.05) with 30 ppm Zn as Zn-gly compared to control. The CMI was sgnificantly (P<0.05) high at 30 ppm Zn supplemented as Zn-gly compared to 40 ppm Zn as ZnSO4 and other organic Zn groups. The SOD activity, humoral and cell mediated immune resonse when fed 20 ppm Zn as Zn-gly was comparable to those on 40 ppm Zn as ZnSO4. The results of the study indicated that reducing the level of Zn from inorganic source (40 ppm) to 50 % (20 ppm) supplementation from Zn-gly can result in comparable immune and antioxidant status in broiler chicks.

Effect of replacing inorganic zinc with a lower level of organic zinc (zinc propionate) on performance, biochemical constituents, antioxidant, immune and mineral status in buffalo calves

“…The CMIR against PHA-P expressed as DTH reaction was higher (P<0.05) in Zn-Prop supplemented calves compared to ZnSO 4 at both 24 and 48h of post sensitization (Table 7). Current findings are in consistent with the results of Sridhar et al (2016) who observed better (P<0.05) cell mediated immunity in Table 7: Cell mediated immune response against PHA-P (increase in skin fold thickness, mm) in buffalo calves fed zinc propionate supplemented diets.…”

Mechanisms Underlying the Protective Effect of Maternal Zinc (ZnSO4 or Zn-Gly) against Heat Stress-Induced Oxidative Stress in Chicken Embryo

“…This is because there are antioxidant protection mechanisms in the body, including a series of antioxidant substances (such as MT) and antioxidant enzymes (such as SOD, GSH-Px, CAT) [ 53 ], and their expression levels represent the antioxidant ability of animal. Studies have confirmed that dietary zinc supplementation can increase the activity of T-AOC, CuZn-SOD, GSH-Px, CAT, T-SOD, and the level of MT in various tissues of chickens [ 34 , 49 , 54 , 55 , 56 ]. Consistent results were also obtained in this experiment, maternal zinc treatment increased the activity of antioxidant enzymes and the level of MT in chicken embryo liver under oxidative stress and non-oxidative stress conditions.…”

Replacement of Inorganic Zinc with Various Organic Zinc Sources on Haematological Constituents, Antioxidant Status, Immune Response and Reproductive Efficiency in Rats

“…Similarly, Nagalakshmi et al, (2015a &2016a) observed better antioxidant activity in rats supplemented with Zn methionine compared to Zn carbonate. Furthermore, Sridhar et al, (2016) observed improved antioxidant status of broiler chicken though reducing the Zn supplementation using organic Zn (Zn glycinate).…”