Effect of Gossypol and Heat on the Digestibility and Utilization of Soybean Protein by Sheep

Effect of free and bound gossypol on the absorption of carbon-14-L-lysine, carbon-14-L-methionine, and carbon-14-L-valine from the rat small intestine

“…This same situation was encountered with lysine when it was used at the actual initial concentrations of methionine and valine, thus necessitating a tenfold decrease in each initial concentration for lysine, a phenomenon also observed by Jervis and Smyth (1959). A decrease in transport at high concentrations has also been noted in the rat gut for L-phenylalanine (Tillman and Kruse, 1962), histidine (Agar et al, 1954), and L-tryptophan (Spencer and Samiy, 1960). Due to the fact that the points for the 10 mM initial con- centrations of methionine for both free and bound gossypol fell away from an asymptotic projection, only the three lowest points representing a 50-fold concentration range were used in the Lineweaver-Burk plots of methionine from which the methionine Kt and Vmax values were derived.…”