Effect of glycosides based standardized fenugreek seed extract in bleomycin-induced pulmonary fibrosis in rats: Decisive role of Bax, Nrf2, NF-κB, Muc5ac, TNF-α and IL-1β

Kandhare, Amit D.; Bodhankar, Subhash L.; Mohan, Vishwaraman; Thakurdesai, Prasad

doi:10.1016/j.cbi.2015.06.019

Cited by 130 publications

(76 citation statements)

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“…Antioxidants are known to inhibit lipid peroxidation by preventing accumulation of the reactive oxygen species. [75][76][77][78][79] In this study, antioxidant activity of FLC decreased lipid peroxidation, controlled abnormal lipid metabolism, and restored altered lipid profile values.…”

Section: Discussionmentioning

confidence: 92%

Flax lignan concentrate reverses alterations in blood pressure, left ventricular functions, lipid profile and antioxidant status in DOCA-salt induced renal hypertension in rats

Sawant

Bodhankar

2016

Renal Failure

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Context Earlier we reported cardioprotective, antihyperlipidemic, and in vitro antioxidant activity of flax lignan concentrate (FLC) obtained from the seeds of Linum usitatissimum L. (Linaceae). Objective To investigate the effect of FLC in deoxycorticosterone acetate (DOCA)-salt induced experimental renal hypertension in rats. Materials and methods Hypertension was induced in uninephrectomized (UNTZD) male Wistar rats (230-280 g) by injecting DOCA (25 mg/kg, subcutaneously, twice weekly) and supplementing 1% NaCl in drinking water for 5 weeks. The rats were divided in six groups. Captopril (30 mg/kg, p.o.) and FLC (200, 400 and 800 mg/kg, p.o.) were administered daily to the rats of groups III-VI, respectively, for 5 weeks. Various hemodynamic and biochemical parameters were investigated as well as histology of kidney and heart were carried out. Results In this study, the FLC (400 and 800 mg/kg) significantly (p50.01, p50.001) decreased the systolic blood pressure, diastolic blood pressure, and mean arterial blood pressure. It also significantly (p50.01, p50.001) decreased elevated end diastolic pressure (EDP), dP/dt max and dP/dt min, organs weights (kidney and heart) and activities of hepatic, renal and cardiac marker enzymes in the serum. Furthermore, FLC (400 and 800 mg/kg) significantly (p50.01, p50.001) restored altered antioxidant status, serum electrolyte level, lipid profile values, and histological abnormalities. Captopril (30 mg/kg) showed maximum antihypertensive effect but low dose of FLC (200 mg/kg) was not enough to show the antihypertensive activity. Conclusion FLC possessed antihypertensive effect via modulation of endogenous enzymes in DOCA-salt induced renal hypertension in rats.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 92%

Flax lignan concentrate reverses alterations in blood pressure, left ventricular functions, lipid profile and antioxidant status in DOCA-salt induced renal hypertension in rats

Sawant

Bodhankar

2016

Renal Failure

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“…Administration of TNBS may disrupt the endogenous antioxidant defense system leading to colonic oxidative damage (Li et al, 2011[47]). Superoxide dismutase (SOD) is an endogenous enzyme, whereas glutathione (GSH), a non-enzymatic antioxidant and both together play a vital role in cellular defense against elevated H 2 O 2 generation (Kandhare et al, 2015[29]). Furthermore, malondialdehyde (MDA) is a highly toxic molecule which is a secondary product of lipid peroxidation.…”

Section: Discussionmentioning

confidence: 99%

“…Hydroxyproline is an amino acid, and its quantification act as an important indicator for collagen synthesis (Kandhare et al, 2015[29]). In the present study, elevated hydroxyproline level was found after TNBS administration, and this increased hydroxyproline level significantly correlated with the accumulated collagen in colonic tissue.…”

Section: Discussionmentioning

confidence: 99%

Ferulic acid ameliorates TNBS-induced ulcerative colitis through modulation of cytokines, oxidative stress, iNOs, COX-2, and apoptosis in laboratory rats

Sadar

Vyawahare

Bodhankar

2016

EXCLI Journal; 15:Doc482; ISSN 1611-2156

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“…), free amino acids (4-hydroxyisoleucine) and individual spirostanols and furastanols like vicenin-1, vitexin, diosgenin, gitogenin, yamogenin, etc. (Bhaskaran & Vishwaraman 2012;Mandegary et al 2012;Kandhare et al 2015b). All these ingredients have a broad spectrum of biological activities including antibacterial, anti-inflammatory, insulinotropic, antidiabetic, hypocholesterolemic, antifertility and anticancer activity (Valette et al 1984;Madar et al 1988;Al-Habori & Raman 1998;Aswar et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of HPLC method for vicenin-1 isolated from fenugreek seeds in rat plasma: application to pharmacokinetic, tissue distribution and excretion studies

Kandhare

Bodhankar

Thakurdesai

2016

Pharmaceutical Biology

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Context: Vicenin-1, a flavonol glycoside, has potent platelet aggregation inhibition, antioxidant, radioprotectants and anti-inflammatory activities. Objective: To establish a rapid, simple, precise and sensitive high-performance liquid chromatography (HPLC) method for determination of vicenin-1 in rat plasma, and to investigate the pharmacokinetics, tissue distribution and excretion after a single 60 mg/kg oral dose in rats. Materials and methods: Vicenin-1 was extracted by solid-liquid extraction through Tulsicon V R ADS-400 (0.40-1.2 mm). Chromatographic separation was achieved by HPLC with a C 18 column with a mobile phase composed of water and acetonitrile (75:25 v/v) and a flow rate of 1 mL/min along with UV detection at 210 nm. Results: Good linearity of calibration curve was found between 10.5 and 100.5 lg/mL (R 2 ¼ 0.995) for plasma and tissue, whereas 2.5-500 lg/mL (R 2 ¼ 0.999) for the urine and stool samples. The extraction recoveries were 98.51-99.58% for vicenin-1 in plasma, whereas intra-day and inter-day precision were validated by relative standard deviation (%RSD), that came in the ranges of 1.16-1.79% and 1.28-1.73%, respectively. The pharmacokinetics results showed C max (7.039 lg/mL) and T max (2 h) after oral administration of vicenin-1. Tissue distribution study showed that the highest concentration of vicenin-1 was achieved in the liver followed by the lung. Approximately 24.2% of its administered dose was excreted via urinary excretion route. Conclusion: The first-pass metabolism, poor solubility and presence of reducing sugar moiety in vicenin-1 may decrease its bioavailability. The developed method is sensitive, specific and was successfully applied to the pharmacokinetics, tissue distribution and excretion studies of vicenin-1 in rats.ARTICLE HISTORY

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Effect of glycosides based standardized fenugreek seed extract in bleomycin-induced pulmonary fibrosis in rats: Decisive role of Bax, Nrf2, NF-κB, Muc5ac, TNF-α and IL-1β

Cited by 130 publications

References 53 publications

Flax lignan concentrate reverses alterations in blood pressure, left ventricular functions, lipid profile and antioxidant status in DOCA-salt induced renal hypertension in rats

Flax lignan concentrate reverses alterations in blood pressure, left ventricular functions, lipid profile and antioxidant status in DOCA-salt induced renal hypertension in rats

Ferulic acid ameliorates TNBS-induced ulcerative colitis through modulation of cytokines, oxidative stress, iNOs, COX-2, and apoptosis in laboratory rats

Development and validation of HPLC method for vicenin-1 isolated from fenugreek seeds in rat plasma: application to pharmacokinetic, tissue distribution and excretion studies

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