Effect of glutathione on sperm quality after short-term cryopreservation in seurukan fish Osteochilus vittatus (Cyprinidae)

Muthmainnah, Cut Ruhul; Eriani, Kartini; Hasri, Iwan; Irham, Muhammad; Batubara, Agung Setia; Muchlisin, Zainal A.

doi:10.1016/j.theriogenology.2018.08.024

Cited by 34 publications

(25 citation statements)

References 33 publications

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“…L-tryptophan acts by transferring an electron to free radicals resulted in the compound becomes stable and protect the plasma membrane injuries. The sperm plasma membrane is able to modulate electrolyte movement at the cellular level appropriately (Muthmainnah et al 2018) in metabolic processes that produce ATP, a sperm energy source (Gonçalves et al 2018;Muthmainnah et al 2018).…”

Section: Resultsmentioning

confidence: 99%

Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan

Kocabaş

Kutluyer

Ertekin

et al. 2019

Systems Biology in Reproductive Medicine

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Experiments were designed to test the influence of L-tryptophan supplementation of the extender on the sperm motility parameters and bacterial flora of brook trout (Salvelinus fontinalis) and rainbow trout (Oncorhynchus mykiss). The extender containing 0.3 M glucose in 10% Dimethyl sulfoxide (DMSO) was supplemented with 0, 0.5, 1, 2, and 4 mM L-tryptophan. Sperm samples stored for up to 12 days at 4°C for brook trout were motile while motility was not observed after six days of storage for rainbow trout. Diluted sperm samples were spread-plated onto Plate Count Agar (PCA) (Total Bacteria Count), Rose Bengal Agar (RBC) (Yeast-Mold Count), Violet Red Bile Dextrose (VRBD) (Enterobacteriaceae count) and Mannitol Salt Agar (MSA) (Micrococcus/ Staphylococcus count) and counts were performed in duplicate and sampling occurred on day 0, 2, 4, 6, and 12 of cold storage. L-tryptophan supplementation of the extender yielded a positive effect, significantly prolonging sperm motility in S. fontinalis and O. mykiss compared to the control group (p < 0.05). The ideal results were obtained above a concentration of 0.5 mM for both species. Total bacteria count in fresh sperm (undiluted samples) was not detectable and not detected in sperm samples treated with different L-tryptophan concentrations. Yeast-mold, Enterobacteriaceae and Micrococcus/Staphylococcus were not detected from fresh and treated sperm brook trout and rainbow trout. On the basis of the results, L-tryptophan-based extender is useful for maintaining sperm quality in short-term storage. L-tryptophan had a negative effect on the bacterial flora. The results of the current study encourages further studies related to longterm storage and reproduction management.

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Section: Resultsmentioning

confidence: 99%

Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan

Kocabaş

Kutluyer

Ertekin

et al. 2019

Systems Biology in Reproductive Medicine

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“…Muthmainnah et al. (2018) reported 63.00 ± 5.89% post‐thaw motility in Osteochilus vittatus sperm with 5% DMSO and 10 min equilibrium time, with a DMSO concentration similar to one used in Plagiognathops microlepis , by Du et al. (2018) who obtained 49 ± 3% motility with 20 min equilibrium.…”

Section: Discussionmentioning

confidence: 97%

“…However, they have variable equilibrium times and different post‐thaw motility percentages between species (Bernáth et al., 2016; Bustamante‐González et al, 2019; Du et al., 2018; Muthmainnah et al., 2018; Nynca et al., 2017).…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Chirostoma jordani sperm, fish model for the conservation of the genus in Mexico

et al. 2020

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Genus Chirostoma belongs to Atherinopsidae family and it is an endemic species from the Mesa Central in Mexico. Abundance of its species have decreased and some ones have been placed on the threatened species list, because of overfishing, urbanization, industrialization, destruction, habitat fragmentation, pollution and exotic species introduction. Chirostoma jordani (Woolman, 1894) is a freshwater fish with biological, ecological, cultural, and commercial importance. It has a broad distribution in Lerma drainage, Durango and Mexico City. In this last locality, their populations, although small, still persist in Xochimilco Lake; it is necessary to implement biotechnologies for their conservation, because of these causes and their basic biology. The aim was to standardize a sperm cryopreservation protocol in C. jordani, to determine extender solution, cryoprotective agent type and concentration, equilibrium time, freezing and thawed rate to be applied in assisted reproduction and conservation of genus Chirostoma. Chirostoma jordani adult males were collected in Atlangatepec Dam, Tlaxcala State, Mexico, to fresh seminal evaluation and cryopreservation protocol standardization. Four cryoprotectants effect was evaluated: dimethylsulfoxide (DMSO), methanol (MeOH), ethylene glycol (EG), and glycerol (GL) at five concentrations: 2%, 6%, 10%, 14% and 16% v/v. Higher and lower DMSO and MeOH 10% and EG 14%, decreased post‐thaw motility percentage. Both DMSO and MeOH 10% and EG 14% had the highest post‐thaw motility percentages, 48.8 ± 1.5%, 54.5 ± 1.0% and 53.5 ± 1.0%, at 15, 10, and 5 min equilibrium times, respectively, thawed at 40°C. Chirostoma jordani sperm can be cryopreserved with both DMSO and MeOH 10%, and EG 14%. These ones can be used for assisted reproduction. GL was not efficient, since it presented a post‐thaw motility percentage very low.

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“…Mystus nemurus [26,38], African catfish Clarias gariepinus [35,[39][40][41][42][43], giant goramy Osphronemus goramy [44,45], lampam Barbonymus gonionotus [46,47], salmonid spermatozoa [48][49][50][51][52], common carp Cyprinus carpio [53][54][55][56][57][58], zebra fish, Danio rerio [59][60][61][62][63] Mozambique tilapia, Sarotherodon mossambicus [64,65], rainbow trout, Oncorhynchus mykiss [66][67][68][69][70][71], and seurukan Osteochillus vittatus [72]. However, cryopreservation of botia sperm has never been reported previously.…”

Section: Several Species Of Fish Sperm Have Been Successfully Cryoprementioning

confidence: 99%

“…Several studies showed that the simultaneously used of both cryoprotectants types gave a better results. For instance, Abinawanto et al [47,80] used a combination of skimmed milk and methanol for Java Barbs, Barbonymous gonionotus, sucrose and methanol for Indonesian Giant Goramy, Osphronemus goramy [44], honey solution, and Dimethyl Sulfoxide (DMSO) for Indonesian Giant Goramy, Osphronemus goramy and Indonesian shark minnow, Osteochillis hasseltii [45,81], and egg yolk and DMSO for depik, Rasbora tawarensis sperm [72]. In this study we tested the combination of honey solution as a natural non-permeating cryoprotectant and methanol as a permeating cryoprotectants with Ringer's solution as an extender.…”

Section: Several Species Of Fish Sperm Have Been Successfully Cryoprementioning

confidence: 99%

The effect of combination of methanol with several concentration of honey solution on sperm quality of botia Chromobotia macracanthus Bleeker 1852 after short-term storage at −80 ℃

Abinawanto

Musthofa

Lestari

et al. 2019

Preprint

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Background: Clown loach or botia, Chromobotia macracanthus Bleeker 1852, is a popular freshwater ornamental fish. Lack of high quality broodstock and asynchronous gonad maturation are the main problem in breeding of this species. Cryopreservation is one of the methods to overcome this problem, and cryoprotectant plays a vital role in this process. Herein, we tested two types of cryoprotectants, namely methanol as a permeating cryoprotectant and honey solution as a natural non-permeating cryoprotectant. Hence, the objective of the present study was to determine best combination of honey solution and methanol for short-term storage of C. macracanthus sperm. Methods: One level of methanol (10%) combined with six levels of honey solution (0%, 0.1%, 0.3%, 0.5%, 0.7% and 0.9%) were tested in this study. Ringer’s solution was used as an extender. The diluted sperms were equilibrated for 25 min at 4 °C and then kept at −80 ℃ for 48 h. Sperms was thawed for 13 s at 40 °C. Spermatozoa viability, motility, and fertilization rates were then observed. Results: The one-way ANOVA showed that combination of methanol with several concentrations of honey had a significant effect on sperm viability and fertilization rates (P<0.05), but did have a significant effect on sperm motility (P>0.05). The study revealed that the 0.1% honey solution combined with 10% methanol resulted in the highest rates of motility (89.4 ± 5.45%), viability (85.75 ± 4.79 %), and fertilization (98.55 ± 1.69 %). Conclusion: A 0.1% honey solution combined with 10% methanol in Ringer’s was the best cryoprotectant for C. macracanthus spermatozoa preserved at -80 ℃.

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Effect of glutathione on sperm quality after short-term cryopreservation in seurukan fish Osteochilus vittatus (Cyprinidae)

Cited by 34 publications

References 33 publications

Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan

Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan

Cryopreservation of Chirostoma jordani sperm, fish model for the conservation of the genus in Mexico

The effect of combination of methanol with several concentration of honey solution on sperm quality of botia Chromobotia macracanthus Bleeker 1852 after short-term storage at −80 ℃

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