Effect of glutathione depletion on the uptake of acrylonitrile vapors and on its irreversible association with tissue macromolecules

Pilon, Denis; Roberts, Alan E.; Rickert, Douglas E.

doi:10.1016/0041-008x(88)90163-9

Cited by 18 publications

(6 citation statements)

References 13 publications

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“…Binding of ACN to these antioxidants, especially GSH, results in the induction of oxidative stress and impaired regeneration of other antioxidants [32]. Studies with 14 C ACN have shown that ACN covalently binds with sulfhydryl groups of protein [38] and to tissue macromolecules and nucleic acids [39]. This explains the reduction in the GSH content of tissues.…”

Section: Discussionmentioning

confidence: 98%

Hesperidin, an antioxidant flavonoid, prevents acrylonitrile‐induced oxidative stress in rat brain

El-Sayed

Abo-Salem

Abd-Ellah

et al. 2008

J Biochem & Molecular Tox

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Acrylonitrile (ACN) is a volatile, toxic liquid used as a monomer in the manufacture of synthetic rubber, styrene plastics, acrylic fiber, and adhesives. ACN is a potent neurotoxin. A role for free radical mediated lipid peroxidation in the toxicity of ACN has been suggested. We examined the ability of hesperidin, an antioxidant flavonoid, to attenuate ACN-induced alterations in lipid peroxidation in rat brains. The daily oral administration of ACN to male albino rats in a dose of 50 mg/kg bwt for a period of 28 days produced a significant elevation in brain lipid peroxides measured as malondialdehyde (MDA) amounting to 107%, accompanied by a marked decrease in brain-reduced glutathione (GSH) content reaching 63%. In addition, ACN administration resulted in significant reductions in the enzymatic antioxidant parameters of brain; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione-S-transferase (GST) recording 43%, 64%, 52%, and 43%, respectively. On the other hand, pretreatment with hesperidin and its coadministration with ACN once daily in a dose of 200 mg/kg bwt i.p. for 28 days ameliorated ACN-induced alterations in brain lipid peroxidation. These results suggest that hesperidin may have a beneficial role against ACN-induced oxidative stress in the brain; an effect that is mainly attributed to the antioxidant property of hesperidin.

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Section: Discussionmentioning

confidence: 98%

Hesperidin, an antioxidant flavonoid, prevents acrylonitrile‐induced oxidative stress in rat brain

El-Sayed

Abo-Salem

Abd-Ellah

et al. 2008

J Biochem & Molecular Tox

View full text Add to dashboard Cite

show abstract

“…Binding of ACN to GSH results in induction of OS and impaired regeneration of other antioxidants 33. Studies with 14 C ACN have shown that ACN covalently binds with sulfhydryl groups of protein 34, macromolecules, and nucleic acids 1. This explains the reduction in the GSH content in cells and increases their susceptibility to OD.…”

Section: Discussionmentioning

confidence: 99%

“…Acrylonitrile (ACN) is widely used in the production of many industrial products such as synthetic fibers, nitrile rubbers, resins, plastics, and pesticide fumigants for grains. It is also used as an intermediate in the synthesis of antioxidants, surface coatings, adhesives, and dyes 1. In clinical practice, ACN is used in the synthesis of high permeable dialysis tubings 2, and artificial membranes to encapsulate Langerhan's islet implants 3.…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective activity of quercetin against acrylonitrile‐induced hepatotoxicity in rats

Abo-Salem

Abd-Ellah

Ghonaim

2011

J Biochem & Molecular Tox

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Acrylonitrile is a potent hepatotoxic, mutagen, and carcinogen. A role for free radical-mediated lipid peroxidation in the toxicity of acrylonitrile has been suggested. The present study was designed to assess the hepatoprotective effect of quercetin against acrylonitrile-induced hepatotoxicity in rats. Liver damage was induced by oral administration of acrylonitrile (50 mg/kg/day/5 weeks). Acrylonitrile produced a significant elevation of malondialdehyde (138.9%) with a marked decrease in reduced glutathione (72.4%), and enzymatic antioxidants; superoxide dismutase (81%), and glutathione peroxidase (53.2%) in the liver. Serum aspartate aminotransferase, alanine aminotransferases, direct bilirubin, and total bilirubin showed a significant increase in acrylonitrile alone treated rats (115.5%, 110.8%, 1006.8%, and 1000.8%, respectively). Pretreatment with quercetin (70 mg/kg/day/6 weeks) and its coadministration with acrylonitrile prevented acrylonitrile-induced alterations in hepatic lipid peroxides and enzymatic antioxidants as well as serum aminotransferases and bilirubin. Histopathological findings supported the biochemical results. We suggest that querectin possess hepatoprotective effect against acrylonitrile-induced hepatotoxicity through its antioxidant activity.

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“…VCN is rapidly absorbed and distributed to all major tissues in animals. Previous studies with 14C have shown that VCN covalently binds to thiol group of proteins (Ahmed et al, 1982) and tissue macromolecules and nucleic acids (Pilon et al, 1988). Therefore, estimation of free radical generation and antioxidant defence has become an important aspect of investigation in mammals.…”

Section: Introductionmentioning

confidence: 99%

Hormonal Physiological Changes of Testis Resulting From Exposure to Vinyl Cyanide and the Possible Protective Role of β-cryptoxanthin in Male Rat

Al-Zail

2021

MJSc

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Vinyl cyanide (VCN) is an aliphatic nitrile product which is extensively used in various synthetic chemical industries. VCN is known to exert toxic actions to human beings as well as experimental animals. The present study was designed to examine the ability of β-cryptoxanthin, a naturally occurring antioxidant, to attenuate VCN-induced testicular toxicity in adult albino rats. Daily oral administration of VCN at a dose level of 30 mg/kg b.w. (7.2mg/ animal) to male rats for a period of 5 days significantly reduced the levels of serum testosterone (T), androsterone, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) which indicates injury to the testis function. Compared to VCN-treated animals, pretreatment with β-cryptoxanthin and its co-administration with VCN once daily at a dose of 40 mg/kg b.w. (9.6mg/ animal) for 30 days induced a remarkable degree of improvement in the levels of endocrine parameters including T, androsterone, FSH and LH. In conclusion, the present results clearly demonstrate the protective role of β-cryptoxanthin against VCN-induced physiological changes in the testis of rats.

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Effect of glutathione depletion on the uptake of acrylonitrile vapors and on its irreversible association with tissue macromolecules

Cited by 18 publications

References 13 publications

Hesperidin, an antioxidant flavonoid, prevents acrylonitrile‐induced oxidative stress in rat brain

Hesperidin, an antioxidant flavonoid, prevents acrylonitrile‐induced oxidative stress in rat brain

Hepatoprotective activity of quercetin against acrylonitrile‐induced hepatotoxicity in rats

Hormonal Physiological Changes of Testis Resulting From Exposure to Vinyl Cyanide and the Possible Protective Role of β-cryptoxanthin in Male Rat

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