Effect of Gibberellic Acid (GA3) and Kinetin on Seed Germination of Sesbania sesban L. and Sesbania rostrata L. (Fabaceae)

Abstract

We investigated the effects of gibberellic acid (GA3) and kinetin on seed germination of S. sesban L. and S. rostrata L. The matured seeds used for this study were obtained from International Institute for Tropical Agriculture (IITA) Ibadan, Nigeria. The seeds were harvested in 2014 and stored dry in a glass container and kept (15°C) in the refrigerator. The viability of the seeds were determined by floating the intact seeds in water and water uptake (imbibition) was carried out. Four replicates of 20 seeds per replicate were germinated and the seeds observed daily and final count was recorded after 14 days of incubation at 30°C. Intact seeds were soaked in gibberellic acid (GA3) and kinetin for 24h and germination percentage taken. The results from the water absorption demonstrated that the seeds of S. rostrata is more permeable compared to S. sesnban as indicated by higher water absorption of seeds of S. rostrata (70%) to S. sesban (25.4%) after 24hrs of incubation. The anatomy of the seed coats indicated the presence of water and gas impermeable tissues namely cuticle, macrosclereids, osteosclereids and disintegrated parenchyma layer. Generally, 0.1 mM kinetin and GA3 enhanced significant germinations compared to the control with 0% germination for the 14 days period of germination. The percentage germination of seeds of S. sesban and S. rotrata subjected to different treatments and germinated in 0.1 mM GA3 and water showed a progressive decrease in germination. From our study, 0.1 mM GA3 and Kinetin significantly enhanced seed germination of S. sesban and S. rostrata.

Salicylic Acid in Root Growth and Development

“…JA activates ABA signaling [110]. Cytokinins and GAs mainly have a stimulating effect on seed germination [91,[104][105][106][107][111][112][113], wherein GAs promote germination activating SA biosynthesis and signaling [114], and cytokinin antagonizes ABA-mediated inhibition of germination by suppressing ABA INSENSITIVE5 (ABI5) [115]. However, in some species, inhibition of radicle emergence upon cytokinin and GAs treatment has been reported [71,116].…”

Factors Affecting Telang (Clitoria ternatea L.) Germination: Systematic Literature Review

“…In combination with scarification, exogenous gibberellin application, for example, is reported to improve germination of several physically dormant legume species such as Mucuna bracteata and Cercis siliquastrum (Grbić et al, 2014;Sari et al, 2014). Meanwhile, the same hormone has also been reported to be able to initiate the germination of intact seeds of two physically dormant legume species, Sesbania rostata and S. sesban (Mensah et al, 2020). Gibberellin also significantly improve the germination of D. regia (Solichatun et al, 2016).…”

“…Meanwhile, internal factors such as seed size and dormancy also affect legume germination (Hill & Auld, 2020;Kuswantoro & Li'aini, 2022). Human intervention, such as dormancy alleviation and hormonal treatments (Kuswantoro & Li'aini, 2022;Mensah et al, 2020), might also affect legume germination in laboratory or agricultural environments. This condition implies that understanding C. ternatea germination biology, including factors affecting the process, is essential to finding an effective propagation method.…”