Effect of gibberellic acid and ethephon on the germination of European beech dormant and chilled beechnuts

Kolářová, Petra; Bezděčková, L.; Procházková, Z.

doi:10.17221/32/2010-jfs

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…A pharmacological approach was adopted to investigate whether changes in the endogenous levels of key regulators of seed germination such as the phytohormones such as ABA, GA and ethylene, as well as ascorbic acid (AA) or hydrogen peroxide (H 2 O 2 ), were involved in the control of the thermoinhibition process. When indicated, seed germination (three plates with 30–35 seeds each) was performed at HT (33°C), in the darkness, in the presence of different concentrations of the ABA biosynthesis inhibitor fluridone, ethephon (acid 2-chlorethylphosphate), which is a source of ethylene in plant tissues (Kolářová et al, 2010 and references therein), GA 4+7 , GA 3 (supplied either alone or in combination with fluridone or ethephon), AA or H 2 O 2 . When indicated, the GA biosynthesis inhibitor paclobutrazol (Hedden and Graebe, 1985) was included in ethephon treatments (see Table 2 for a description of all the conditions tested).…”

Section: Methodsmentioning

confidence: 99%

Germination responses in Zephyranthes tubispatha seeds exposed to different thermal conditions and the role of antioxidant metabolism and several phytohormones in their control

Acosta

Manfreda²,

Alcaraz³

et al. 2022

Seed Sci. Res.

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Zephyranthes tubispatha is an ornamental species distributed along several countries of South America. Although it can be multiplied through bulbs or scales, seed germination is a simpler and more cost-effective process. Temperature plays a major role in the control of germination; however, its effect has been scarcely investigated in this species. In the present work, we characterized the germination responses of Z. tubispatha seeds to different temperatures and analyzed the role of key components of the antioxidant metabolism and phytohormones in their control. Seeds showed an optimal temperature range for germination between 14 and 20°C, with higher temperatures (HTs) being progressively inhibitory. While germination was almost nil above 28°C, it could be recovered after transferring the seeds to 20°C, suggesting that thermoinhibition was the underlying phenomenon. The duration of the HT incubation period affected both the time to germination onset and the germination rate at 20°C. Similarly, the activity of antioxidant enzymes, the production of reactive oxygen species in the embryo and the sensitivity to some germination promoters varied depending on the duration of the HT treatment. The addition of 20 μM fluridone was sufficient to recover germination dynamics as in the control treatment when given after a long-term incubation period (25 d) at HT. Ethephon supply was more effective than gibberellins to suppress thermoinhibition, suggesting that changes in the balance and/or sensitivity to ethylene and abscisic acid over time play an important role in the regulation of germination responses to thermal cues in this species.

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Section: Methodsmentioning

confidence: 99%

Germination responses in Zephyranthes tubispatha seeds exposed to different thermal conditions and the role of antioxidant metabolism and several phytohormones in their control

Acosta

Manfreda²,

Alcaraz³

et al. 2022

Seed Sci. Res.

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“…Dormancy is determined genetically but it can be regulated by environmental factors. The techniques for breaking the dormancy of beechnuts have been improved using a variety of treatments (chemical or physical) (Gendreau and Corbineau 2009;Kolářová et al 2010). Many substances controlling seed viability and dormancy are associated with germination capacity.…”

Section: Introductionmentioning

confidence: 99%

Storage compounds, ABA and fumarase in Fagus sylvatica embryos during stratification

Eliášová¹,

Pešek²,

Vondráková³

2015

Dendrobiology

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Understanding the mechanisms of seed dormancy is essential for planning optimal seed storage and for breaking the dormancy of stored seeds. Our experiments focused on three aspects of characterization of breaking the dormancy of Fagus sylvatica seeds during moist low temperature stratification. 1) We used the changes of ABA content in embryos during the whole process of stratification as the marker of the depth of dormancy: the decrease in the depth of dormancy (according standard germination tests) was directly proportional to the drop in endogenous ABA content in both cotyledons and embryonic axes. 2) Fumarase activity (the second marker of dormancy) increased gradually during stratification.3) The histochemical visualisation of storage compounds as the third marker of dormancy documented the changes in storage protein deposition during stratification. The proteinaceous content of storage vacuoles disappeared; the vacuoles diminished, petered out or fused, creating central vacuoles. This process commenced with imbibition of the seeds and it was more pronounced in the cells in the external parts of the cotyledons. No changes in the size and location of starch grains or calcium oxalate crystals linked with stratification were detected.

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“…On the other hand, inappropriate chilling may cause germination to decrease. Gibberellic acid (GA3) could possibly substitute for chilling (Kolářová et al 2010). Gibberellins are the part of phytohormones which have multifunctional effects on the regulation of ontogenesis.…”

mentioning

confidence: 99%

Effect of gibberellic acid on germination capacity and emergence rate of Sycamore maple (Acer pseudoplatanus L.) seeds

Stejskalová¹,

Kupka²,

Miltner³

2015

J. For. Sci.

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Seedlots of sycamore maple (Acer pseudoplatanus L.) originating from three different provenance regions were collected in autumn 2012. The seed quality, namely vitality and germination rate, was assessed according to Czech conventions. There were four replications within each seedlot, i.e. twelve treatments altogether for each experimental variant. The effect of gibberellic acid on germination and vitality was studied using three treatments including a control for germination capacity and three treatments including a control for emergence rate. The data shows that the gibberellic acid did not substitute for the stratification procedure as it did not improve the germination capacity of seeds with pericarp when compared with stratified seeds; however for seeds without pericarp the gibberellic acid improved the germination capacity to the level of stratified seeds. The data also shows the positive influence of gibberellic acid on emergence rate. All variants where acid gibberellic was applied have a statistically higher emergence rate than the control. The increase was about 50% higher than in the control, i.e. without the influence of gibberellic acid.

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Effect of gibberellic acid and ethephon on the germination of European beech dormant and chilled beechnuts

Cited by 4 publications

References 6 publications

Germination responses in Zephyranthes tubispatha seeds exposed to different thermal conditions and the role of antioxidant metabolism and several phytohormones in their control

Germination responses in Zephyranthes tubispatha seeds exposed to different thermal conditions and the role of antioxidant metabolism and several phytohormones in their control

Storage compounds, ABA and fumarase in Fagus sylvatica embryos during stratification

Effect of gibberellic acid on germination capacity and emergence rate of Sycamore maple (Acer pseudoplatanus L.) seeds

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