Effect of Germination, under Different Environmental Conditions, on Saponins, Phytic Acid and Tannins in Lentils (Lens culinaris)

Abstract

The e †ect of germination conditions on some antinutrients of L ens culinaris var Magda 20 seeds were studied. The seeds were germinated at 20¡C under variable conditions of time, water and light. Quantitative analyses of the soyasapogenols, inositol phosphates and tannins were carried out by capillary gas chromatography, high-performance liquid chromatography and spectrophotometric techniques respectively. Germinated seeds at day 6 contained higher levels soyasapogenol B than the controls, whereas in general the tannin content was reduced. Total phytic acid amounts did not decrease after 3 days of germination but was greatly reduced after 6 days. This work shows that the optimal conditions to reduce some antinutritional factors (tannins and phytic acid) in lentils were 6 days of seed germination in dark and with alternate watering. Therefore, germination conditions o †er a good opportunity to improve the nutritional quality of lentils.

Effect of Processing on Antinutrient Compounds in Pulses

“…The soyasapogenol B and soyasaponin I contents increased during six days of germination in lentils (Ayet et al 1997), contrary to other reports in which the germination process reduced these compounds in chickpeas and black gram (Jood et al 1986). The soyasapogenol B and soyasaponin I contents increased during six days of germination in lentils (Ayet et al 1997), contrary to other reports in which the germination process reduced these compounds in chickpeas and black gram (Jood et al 1986).…”

“…The pulse type, germination conditions (growing under light or dark conditions, timing and amount of water addition), and duration of germination (1-10 days) affect antinutrient content, thus creating difficulty when comparing reported antinutrient values. Systematic studies were conducted by Ayet et al (1997) on lentils and Vidal-Valverde and coworkers (2002) on peas, lentils, and edible beans. Optimal conditions to reduce tannins and phytic acid in lentils were found to be six days of germination in the dark with alternate watering by Ayet's laboratory.…”

Phytate in foods and significance for humans: Food sources, intake, processing, bioavailability, protective role and analysis

“…Similar results were reported by Wing [255] who determined the fractional cadmium accumulation ( 109 Cd) from different diets based on either whole wheat, bran or endosperm with different levels of phytate (4.2, 7.6 and 0.3 mmol IP 6 /kg, respectively). The fractional accumulation of 109 Cd in the liver and the kidneys was significantly lower (p a 0.001) in rats fed the phytate rich whole wheat and bran diets compared to the low phytate endosperm diet (control diet). The bioavailability of cadmium from cow's milk formula, soy formula, wheat/oat/milk formula, whole meal/milk formula and water were compared in rat pups [256].…”

Iterative operator splitting method for capillary formation model in tumor angiogenesis problem: Analysis and application

“…A reduced phenolic content in cereals and legumes has been reported after soaking in different media,7, 8 cooking,10, 11 germination12–16 and fermentation 12, 13, 17, 18. The reduced amounts of phenolic compounds as a result of such treatments might be attributed to several factors.…”

“…These compounds form complexes with food nutrients, making the latter either less soluble or less susceptible to enzymatic degradation and thus less available for absorption. Community‐based processing/treatment techniques including soaking in different media,7–9 cooking,10, 11 germination12–16 and fermentation12, 13, 17–20 have been shown to reduce tannin levels in grains to various extents.…”