Effect of Gentamicin and Chloramphenicol on Bovine Lens Optical Function During Culture

Sivak, J. G.; Yoshimura, Minoru; Weerheim, J.

doi:10.3109/15569529009036332

Cited by 22 publications

(15 citation statements)

References 17 publications

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“…The temperature of the culture dish did not exceed 37°C. Lens optical quality measurements were carried out as described previously [24]. For each time interval of control and irradiated lenses, we used 4 young and 4 adult lenses, respectively.…”

Section: Methodsmentioning

confidence: 99%

Analysis of UVA-Related Alterations upon Aging of Eye Lens Proteins by Mini Two-Dimensional Polyacrylamide Gel Electrophoresis

Weinreb

Rijk²,

Steely³

et al. 2000

Ophthalmic Res

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This study is a first approach to identify UVA-related alterations in situ of bovine eye lens proteins from the water-soluble and urea-soluble fractions upon aging. The fractions were obtained from irradiated long-term organ culture lenses and analyzed by mini two-dimensional gel electrophoresis. This micropreparative method followed by computer analysis allows high resolution and separation of microgram quantities of proteins and to detect spots which arose as a consequence of irradiation. To facilitate the analysis we first separated the water-soluble fraction into the major crystallin classes by gel filtration. Moreover, we immunoblotted the gel of the urea-soluble fraction with a specific antibody against the intermediate filament protein vimentin. Upon irradiation of young and adult lenses, αA-crystallin and vimentin showed obvious modifications. During aging the susceptibility to irradiation increased when vimentin started to degrade, whereas deamidation of αA-crystallin seems to occur.

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Section: Methodsmentioning

confidence: 99%

Analysis of UVA-Related Alterations upon Aging of Eye Lens Proteins by Mini Two-Dimensional Polyacrylamide Gel Electrophoresis

Weinreb

Rijk²,

Steely³

et al. 2000

Ophthalmic Res

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“…Each lens was located in the culture chamber, leaving a clear space filled with culture medium both below and above the lens; this medium was changed daily. An optical bench [Sivak et al, 1990] was used for the daily test of both exposed and control lenses. A 670 nm diode laser with the beam parallel to the axis of the lens was directed towards the cultured lens along one meridian in 0.5 mm increments.…”

Section: Optical Monitoring Systemmentioning

confidence: 99%

Localized effects of microwave radiation on the intact eye lens in culture conditions

Dovrat

Berenson

Bormusov

et al. 2005

Bioelectromagnetics

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A novel experimental system was used to investigate the localized effects of microwave radiation on bovine eye lenses in culture for over 2 weeks. Using this setup, we found clear evidence that this radiation has a significant impact on the eye lens. At the macroscopic level, it is demonstrated that exposure to a few mW at 1 GHz for over 36 h affects the optical function of the lens. Most importantly, self-recovery occurs if the exposure is interrupted. At the microscopic level, close examination of the lens indicates that the interaction mechanism is completely different from the mechanism-causing cataract via temperature increase. Contrary to the latter's effect, that is particularly pronounced in the vicinity of the sutures and it is assumed to be a result of local friction between the edges of the fibers consisting the lens. Even if macroscopically the lens has recovered from the irradiation, microscopically the indicators of radiation impact remain.

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“…Previously, changes in optical quality of the vertebrate crystalline lens in vitro have been used as the outcome measure in response to a variety of chemicals (Sivak et al, 1990(Sivak et al, , 1995Dorfman-Hecht et al, 1994 ;Herbert and Sivak, 1996). The optical measures are objective and easily quantified.…”

Section: Introductionmentioning

confidence: 99%

Optical Properties of Rainbow Trout Lenses after in vitro Exposure to Polycyclic Aromatic Hydrocarbons in the Presence or Absence of Ultraviolet Radiation

Laycock

Schirmer

Bols

et al. 2000

Experimental Eye Research

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Effect of Gentamicin and Chloramphenicol on Bovine Lens Optical Function During Culture

Cited by 22 publications

References 17 publications

Analysis of UVA-Related Alterations upon Aging of Eye Lens Proteins by Mini Two-Dimensional Polyacrylamide Gel Electrophoresis

Analysis of UVA-Related Alterations upon Aging of Eye Lens Proteins by Mini Two-Dimensional Polyacrylamide Gel Electrophoresis

Localized effects of microwave radiation on the intact eye lens in culture conditions

Optical Properties of Rainbow Trout Lenses after in vitro Exposure to Polycyclic Aromatic Hydrocarbons in the Presence or Absence of Ultraviolet Radiation

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