Effect of genotype and medium composition on linseed (Linum usitatissimum) ovary culture

Abstract

Breeding linseed (Linum usitatissimum L.) using haploid techniques allows breeders to develop new cultivars in a shorter time period. Many research groups successfully created new linseed genotypes through anther culture; however ovary culture has been the subject of only a few earlier studies. In the present study, the effect of genotype and growth regulators combination on callus induction and shoots regeneration in ovary culture of nine commercially important linseed cultivars was investigated. Ovaries were cultured on modified MS medium supplemented with three different combinations of plant growth regulators. Variable callogenic responses were expressed by all of the genotypes tested on different induction media. The results suggested that specific combination of growth regulators for callus induction must be designed for each genotype. Shoot regeneration from ovary derived callus is a critical phase of the whole gynogenetic process. Differences in adventitious shoot formation frequency among genotypes were demonstrated and four responsive genotypes have been selected. Ovary derived callus from cultivar 'Mikael' manifested the highest adventitious shoot formation frequency with a high number of shoots per explant. The optimum ratio of growth regulators for shoot regeneration was shown to depend on the genotype. Cultivars 'Linola', 'Mikael' and 'Szaphir' showed the highest shoot regeneration frequency when callus had originated on induction medium supplemented with 2 mg L −1 BAP and 2 mg L −1 NAA, while combination of 1 mg L −1 BAP and 2 mg L −1 IAA promoted shoot formation in ovary-derived callus of 'Barbara'. The highest rate of shoots per explant has been obtained in second subculture.

A Novel Regeneration System for Tamarillo (Cyphomandra betacea) via Organogenesis from Hypocotyl, Leaf, and Root Explants

“…bud formation from explants were reported by Zaffari et al (2000) and Subramaniam et al (2008) in Cavendish banana cultivar Brasilian (AAA). However, the results are contrary to many reports that indicate that there is reduction in number of shoots in media supplemented with cytokinins concentrations higher than optimal level (Burbulis et al, 2011;Rai et al, 2009). The root explants cultured on media supplemented with BA in all the different concentrations did not produce any microshoots.…”

Comparative analysis of fatty acid composition in 84 accessions of flax (Linum usitatissimum L.)

“…Flax (Linum usitatissimum L.) is one of the oldest cultivated plants in the world [1]. It is a dual-purpose crop, a source of seeds and fiber [2]. Some genotypes are grown mainly for fiber (fiber -type of use), others mainly for seeds (linseed -type of use) and some for fiber and seeds together (combined -type of use).…”

Performance of Frontline Demonstration on Productivity and Profitability of Utera Cropping of Linseed (Linum usitatissimum L.) under Rainfed Conditions in Tribal District Balaghat of Madhya Pradesh, India

“…It is tolerance to biotic and abiotic stresses, which makes it suitable for a wide range of tropical, sub-tropical and temperate regions. It is a dualpurpose crop and is grown for seeds and fibre which is used for the manufacture of linen" [2]. "The oil content of the seed varies from 33-47%.…”

“…"The productivity of oilseed in the district is low as compared to the national average mainly due to poor crop management practices and inadequate availability of quality seeds of improved Linseed varieties and other inputs. To increase the productivity of linseed in the Balaghat district an adequate and balance supply of plant nutrients along with high-yielding varieties is of critical importance" [2]. Employing a strategic approach, the systematic conduct of Front-line Demonstrations (FLDs) directly on farmers' fields proves to be an effective tool for extension, facilitating the unspoiled transfer of knowledge and skills among peers.…”