Effect of genistein on proinflammatory cytokines and estrogen receptor–β in mice model of endometriosis

The purpose of this study was to investigate the effect of genistein administration on the modulation of the estrogen receptor, inhibition of inflammation and angiogenesis in the murine model of peritoneal endometriosis. A total of thirty-six mice (Mus musculus) were divided into six groups (n = 6), including the control group, endometriosis group, endometriosis group treated with various doses of genistein (0.78; 1.04; 1.3 mg/day), and endometriosis group treated with leuprolide acetate (0.00975 mg/day every 5 days for 15 days). Analysis of estrogen receptor-α, estrogen receptor-β, TNF-α, IL-6, VEGF, and HIF-1α were performed immunohistochemically. Expression of estrogen receptor-α, estrogen receptor-β, TNF-α, IL-6, VEGF and HIF-1α increased significantly compared with the control group (p < 0.05). All doses of genistein decreased the expression of estrogen receptor-α, increased estrogen receptor-β, lowered VEGF and HIF-1α significantly compared with endometriosis group (p > 0.05). Genistein also decreased the expression of TNF-α and IL-6 (1.04 and 1.3 mg/day) compared with the endometriosis group, reaching level comparable to that of the control group (p > 0.05). It was concluded that genistein is able to modulate estrogen receptor-α and estrogen receptor-β and inhibit the development of inflammation and angiogenesis in the murine model of peritoneal endometriosis. Thus, genistein can be a candidate in the treatment of endometriosis.

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“…These results are consistent with previous studies, saying that genistein inhibited neo-angiogenesis through the inhibition of HIF-1α. 43 , 32 …”

Section: Discussionmentioning

confidence: 99%

“…The development of endometriosis in mice was observed for 14 days and categorized as a model of endometriosis. 32 …”

Section: Methodsmentioning

confidence: 99%

Genistein modulates the estrogen receptor and suppresses angiogenesis and inflammation in the murine model of peritoneal endometriosis

Sutrisno

Aprina

Simanungkalit

et al. 2018

Journal of Traditional and Complementary Medicine

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“…However, rats given oral genistein in Yavuz et al [ 166 ] saw a significant regression of peritoneal endometriotic implants when compared to the control group. These two studies suggest genistein might have an inhibitory and even regressive effect on endometriotic cells, which is supported by studies showing that genistein inhibits expression of proinflammatory cytokines NF-κB, ESR2 [ 167 ], Bcl-2, COX-2, and PGE [ 168 ] in rodent models of endometriosis. There does not appear to be thorough research into why rodent studies are more conclusive in supporting genistein’s potential for treating endometriosis, but it may be due to several factors ranging from the paucity of human investigations, fundamental physiological differences between humans and rodents, confounding lifestyle variables, and more.…”

Section: Genistein and Women’s Diseasesmentioning

confidence: 82%

Genistein: Dual Role in Women’s Health

Yu¹,

Ríos²,

Castro³

et al. 2021

Nutrients

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Advanced research in recent years has revealed the important role of nutrients in the protection of women’s health and in the prevention of women’s diseases. Genistein is a phytoestrogen that belongs to a class of compounds known as isoflavones, which structurally resemble endogenous estrogen. Genistein is most often consumed by humans via soybeans or soya products and is, as an auxiliary medicinal, used to treat women’s diseases. In this review, we focused on analyzing the geographic distribution of soybean and soya product consumption, global serum concentrations of genistein, and its metabolism and bioactivity. We also explored genistein’s dual effects in women’s health through gathering, evaluating, and summarizing evidence from current in vivo and in vitro studies, clinical observations, and epidemiological surveys. The dose-dependent effects of genistein, especially when considering its metabolites and factors that vary by individuals, indicate that consumption of genistein may contribute to beneficial effects in women’s health and disease prevention and treatment. However, consumption and exposure levels are nuanced because adverse effects have been observed at lower concentrations in in vitro models. Therefore, this points to the duplicity of genistein as a possible therapeutic agent in some instances and as an endocrine disruptor in others.

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“…[54][55][56][57] Sutrisno et al in their investigation demonstrated that genistein as an inhibitor of Wnt/bcatenin signaling pathway decreased high levels of IL-6 and IL-8 in mice model of endometriosis and in vitro cultured endometriotic cells. 58,59 Additionally, another study showed that artemisinin with anti-inflammatory properties could suppresses mRNA expression of IL-6 in chondrocytes extracted from patients with osteoarthritis through the interference with Wnt/b-catenin signaling pathway. 60 Delbandi et al revealed that the 1,25(OH)2D3, a Wnt/b-catenin signaling pathway inhibitor, 61 could suppress the IL-6 secretion by endometriotic stromal cells isolated from women with ovarian endometriosis, but this vitamin had no significant effect on IL-8 production.…”

Section: Discussionmentioning

confidence: 99%

Pyrvinium pamoate induces in-vitro suppression of IL-6 and IL-8 produced by human endometriotic stromal cells

et al. 2020

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Endometriosis, a chronic inflammatory disease, is identified by the presence of endometrial tissue outside the uterus. The prevalence of this disease among reproductive-age women is almost 10–15%. High levels of IL-6 and IL-8 have been found in the peritoneal fluid (PF) of women with endometriosis and are involved in its pathogenesis. Isolated stromal cells from 12 ectopic and eutopic endometrial biopsies of women with ovarian endometrioma and also 12 endometrial biopsies of nonendometriotic controls were treated with 1.1 µM pyrvinium pamoate, a Wnt/β-catenin signaling pathway inhibitor, for 72 hrs. Before treatment, mRNA gene expression and secretion of IL-6 and IL-8 were significantly higher in ectopic (EESCs) than eutopic (EuESCs) and control (CESCs) endometrial stromal cells. After treatment, mRNA gene expression and also secretion of IL-6 and IL-8 were significantly reduced. Our Findings showed that pyrvinium pamoate suppresses the mRNA gene expression and secretion of IL-6 and IL-8 in human endometriotic stromal cells. Additional investigations on this compound are required before clinical application.

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Effect of genistein on proinflammatory cytokines and estrogen receptor–β in mice model of endometriosis

Cited by 10 publications

References 13 publications

Genistein modulates the estrogen receptor and suppresses angiogenesis and inflammation in the murine model of peritoneal endometriosis

Genistein modulates the estrogen receptor and suppresses angiogenesis and inflammation in the murine model of peritoneal endometriosis

Genistein: Dual Role in Women’s Health

Pyrvinium pamoate induces in-vitro suppression of IL-6 and IL-8 produced by human endometriotic stromal cells

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