Effect of gelling agents on in vitro development of Amelanchier canadensis ‘Rainbow Pillar’

Abstract

In vitro shoot multiplication responses of Amelanchier canadensis ‘Rainbow Pillar’ were studied on media solidifi ed with different gelling agents. The media were gelled either with 6.8 g l-1 fi brous agar-agar, or 50.0 g l-1 wheat starch, or 20.0 g l-1 Guar gum, or 15 g l-1 Isubgol or 50.0 g l-1 wheat starch mixed with 0.5 g l-1 Phytagel. Shoot cultures were grown for two months, thereafter the multiplication rates (number of newly developed shoots per explant) were counted and the length of shoots were measured. We found that the highest shoot multiplication of Amelanchier canadensis ‘Rainbow Pillar’ occurred on media gelled with Guar gum, while the longest shoots developed on media with Starch. About four-fold shoot number were obtained on media with Guar gum compared to the weakest results found on media gelled with Isubgol. Finally, considering all factors (shoot growth parameters, costs) the most economical gelling agent for Amelanchier canadensis ‘Rainbow Pillar’ was proved to be wheat starch among the tested alternatives which allows a 75.6% cost reduction.

The Use of Wheat Starch as Gelling Agent for In Vitro Proliferation of Blackberry (Rubus fruticosus L.) Cultivars and the Evaluation of Genetic Fidelity after Repeated Subcultures

“…Furthermore, micropropagation strategies aim to achieve two goals simultaneously: to increase the proliferation rate of the targeted plant material and to reduce production costs at the same time. Replacing agar, the most expensive component of the culture medium, with alternative low-priced gelling agents can lead to reduced production costs of the in vitro cultures [64].…”

“…If the medium is excessively soft, it can lead to hyperhydricity in tissue cultures, while if it is excessively hard, it can impede shoot development. [64].…”

Establishment of an Efficient Micropropagation System for Humulus lupulus L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers

“…Gelling agents, especially Plant agar is a relatively expensive component of in vitro culture media, and some cheaper alternatives were found with beneficial effects in the micropropagation process of some species [57]. Wheat starch, for example, has been used in Goji berry and blackberry in vitro culture and provided not only higher multiplication rates as compared to media gelled with Plant agar but also a high quality of the regenerated shoots [58,59].…”

Особливості насінного розмноження інтродукованих представників роду Amelanchier Medik

“…Інтродукція Amelanchier spp., як і більшості інших рослин, у минулому й дотепер здійснюється переважно через насіння, що пояснює інтерес щодо вивчення особливостей їхнього насінного розмноження. У численних публікаціях висвітлюються особливості збирання плодів, виділення й зберігання насіння (Kuklina, 2011;Strela, 1970); аналізуються варіанти стратифікації та інших способів підготовлення його до сівби (Brinkman & Stron, 2008;Dirr & Heuser, 2012), зокрема через згодовування плодів птахам і тваринам з проходженням насіння крізь травний тракт (Kuklina, 2011;Robinson, 1986), а також вивчаються строки сівби; можливості виведення з органічного спокою (Bewley et al, 2013;Cruz-Cruz, 2005;Kuklina, 2011;Nikolaeva et al, 1985), пророщування насіння в лабораторних умовах (Strela, 1970); зокрема використання технологій in vitro (Fira et al, 2013;Koldar et al, 2015;Pinker & Oellerich, 2007;Yang & Du, 2017).…”