Effect of garlic on isoniazid and rifampicin-induced hepatic injury in rats

Abstract

AIM: To evaluate the hepatoprotective effect of garlic on liver injury induced by isoniazid (INH) and rifampicin (RIF). METHODS:Wistar rats weighing 150-200 g were treated orally with 50 mg/kg of INH and RIF daily each for 28 d. For hepatoprotective studies, 0.25 g/kg per day of freshly prepared garlic homogenate was administered orally half an hour before the INH+RIF doses. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and bilirubin were estimated on d 0, 14, 21, and 28 in all the rats. Histological analysis was carried out to assess the injury to the liver. Lipid peroxidation (LPO) as a marker of oxidative stress and non-protein thiols (glutathione) for antioxidant levels were measured in liver homogenate. RESULTS:The treatment of rats with INH+RIF (50 mg/kg per day each) induced hepatotoxicity in all the treated animals as judged by elevated serum ALT, AST, and bilirubin levels, presence of focal hepatocytic necrosis (6/8) and portal triaditis (8/8). Garlic simultaneously administered at a dose of 0.25 g/kg per day prevented the induction of histopathological injuries in INH+RIF co-treated animals, except in 4 animals, which showed only moderate portal triaditis. The histological changes correlated with oxidative stress in INH+RIF treated animals. The group which received 0.25 g/kg per day garlic homogenate along with INH+RIF showed higher levels of glutathione (P < 0.05) and low levels of LPO (P < 0.05) as compared to INH+RIF treated group.

Effect of tylophora asthmatica on hepatotoxicity induced by antitubercular drugs in albino rats

“…18 In another study by Pal et al it was seen that the treatment with (INH 50 mg/kg+ RIF 50 mg/kg) caused portal triaditis and liver necrosis. 19 In the present study administration of antitubercular drugs resulted in portal inflammation, ballooning degeneration, fatty change and necrosis in rat liver, which supports the findings of previous studies. 18,19 In the present study it was also found that there were no significant changes like portal inflammation, ballooning degeneration, fatty change and necrosis in groups treated with aqueous extract of tylophora asthmatica for 14 days.…”

Modulatory Activity of Bee Pollen Against the Toxicity of Antituberculosis Drugs Rifampicin and Isoniazid in Testis of Sprague Dawley Rats

“…Bee pollen has been found to have some protective effect against carbon tetrachloride-induced toxicity [17] and malathioninduced toxicity [18]. Previous studies showed that antituberculosis drugs damaged the endogenous antioxidant system of the whole body [3][4][5][6][7] In our results, it is clearly reflected that supplementation of diet with bee pollen in animals has antioxidant and protective effect when given along with antituberculosis drugs (rifampicin as well as isoniazid). It is thus observed that bee pollen has strong potential to provide protection against antituberculosis drugs-induced toxicity in rats as evidenced by improved histological parameters and rebuilding of the antioxidant/oxidative status.…”

“…About 100 g of bee pollen contained about 396.4-411.1 kcal energy [16]. Rifampicin-and isoniazid-induced toxicity and oxidative stress relationship have been reported previously [3,4,7]. Mechanism of drug toxicity is through production of free radical and their capability to induce apoptosis by the production of ROS, lipid peroxidation, and cell membrane damage [5,6].…”

“…To minimize the adverse effects of tuberculosis therapy, there is a necessity to supplement tuberculosis therapy with natural products. Various natural products such as Ocimum sanctum [3], garlic [4], Silymarin [5], Aloe vera [6], and propolis [7] had potential to reduce the oxidative stress caused by antituberculosis drugs. Keeping all these activities in mind, the present experiment was designed to study the preventive role of bee pollen against toxic effect of antituberculosis drug induced toxicity in Sprague Dawley (SD) rats.…”

Hepatoprotective effect of <i>Monotheca buxifolia</i> fruit against antitubercular drugs-induced hepatotoxicity in rats

“…Suspension of isoniazid and rifampicin were separately prepared and given to rats in dose of 50 mg/kg each for 21 days via oral gavage tube (Pal et al, 2006).…”