Effect of Gamma Rays onSophora davidiiand Detection of DNA Polymorphism through ISSR Marker

Abstract

Sophora davidii (Franch.) Kom. ex Pavol is an important medicinal plant and a feeding scrub with ecological value. The effects of different gamma irradiation doses (20–140 Kr) on seed germination and seedling morphology were investigated in S. davidii, and intersimple sequence repeat (ISSR) markers were used to identify the DNA polymorphism among mutants. Significant variations were observed for seed germination, stem diameter, and number of branches per plant. The improved agronomic traits, such as stem diameter and number of branches per plant, were recorded at 80 Kr dose and 20 Kr dose for seed germination. ISSR analysis generated in total 183 scorable fragments, of which 94 (51.37%) were polymorphic. The percentage of polymorphism ranged from 14.29 to 93.33 with an average of 45.69%. Jaccard's coefficients of dissimilarity varied from 0.6885 to 1.000, indicative of the level of genetic variation among the mutants. The constructed dendrogram grouped the entities into five clusters. Consequently, it was concluded that gamma rays irradiation of seeds generates a sufficient number of induced mutations and that ISSR analysis offered a useful molecular marker for the identification of mutants.

Morphological, cytological and molecular variations induced by gamma rays in Chrysanthemum morifolium ‘Donglinruixue’

“…It was revealed that DNA changes had occurred in these materials and the dendrogram, showing the formation of five main groups of mutants, and that indicated the effects of different irradiation dosages on seedlings are far from each other. This result was in accordance with the studies on Sophora davidii (Wang et al, 2017), lily (Xi et al, 2012) and banana (Khan, 2011). Also, there is a genetic gap between dwarfing plants and other types of variation, and it can be concluded that the dwarfing plants have a genetic level change that is of great significance for obtaining dwarfing varieties of ground-grown chrysanthemum and the mutants were successfully identified through ISSR analysis.…”

Field Evaluation and Molecular Analysis of Three Early Flowering Canola Mutants under Natural Salinity-Stressed Environment

“…This study showed that the mutants and their parent and the check possess a moderate polymorphism as well as revealed by Xi et al (2012) and Wang et al (2017) who also detected a moderate polymorphism among gamma rayinduced mutants. The appearance or disappearance of bands as specific for γ-ray induced mutants is may be attributed to DNA structural rearrangements caused by different types of DNA damages (Selvi et al, 2007 andMejri et al, 2012).…”

The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker

“…Detection of genetic diversity using molecular markers of ISSR has been carried out in several previous studies (Wang et al, 2012;Patel et al, 2015;Taheri et al, 2013;Wang et al, 2017). Single nucleotide polymorphisms are the genetic markers of choice in various genetic, ecological and evolutionary studies (Tang et al 2016).…”

“…Besides plant material, primary selection is also very important. Wang et al (2017) in his research on the Sophora davidii plant stated that the induction of gamma radiation was able to produce genetic diversity between 75-86% based on the ISSR marker. Another study conducted by Taheri et al (2013) on Curcuma alismatifolia plants based on ISSR analysis results of gamma-ray irradiation obtained diversity between 27-100%.…”