Effect of fusaric acid on the leaf physiology of cucumber seedlings

Wang, Min; Ling, Ning; Dong, Xian; Liu, Xiaokang; Shen, Qirong; Guo, Shiwei

doi:10.1007/s10658-013-0306-4

Cited by 30 publications

(24 citation statements)

References 50 publications

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“…The results indicate that the leaves of FA treated plants accumulated more red ink and had a higher relative electronic conductance ( Fig. 5 , Table 4 ), in agreement with our previous study with transmission electron microscopy (TEM), in which the leaf mesophyll cell membrane was seriously injured after treatment with FA 54 . The ratio of the transpiration rate to the stomatal conductance (E/g s ) was increased in the FA-treated plants, indicating that water had evaporated from damaged cells, which is caused by FA movement from the infection site to the leaf.…”

Section: Discussionsupporting

confidence: 91%

Water balance altered in cucumber plants infected with Fusarium oxysporum f. sp. cucumerinum

Wang

Sun

et al. 2015

Sci Rep

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Fusarium wilt is caused by the infection and growth of the fungus Fusarium oxysporum in the xylem of host plants. The physiological responses of cucumbers that are infected with Fusarium oxysporum f. sp. cucumerinum (FOC) was studied in pot and hydroponic experiments in a greenhouse. The results showed that although water absorption and stem hydraulic conductance decreased markedly in infected plants, large amounts of red ink accumulated in the leaves of infected cucumber plants. The transpiration rate (E) and stomatal conductance (gs) of the infected plants were significantly reduced, but the E/gs was higher than healthy plants. We further found that there was a positive correlation between leaf membrane injury and E/gs, indicating that the leaf cell membrane injury increased the non-stomatal water loss from infected plants. The fusaric acid (FA), which was detected in the infected plant, resulted in damage to the leaf cell membranes and an increase in E/gs, suggesting that FA plays an important role in non-stomatal water loss. In conclusion, leaf cell membrane injury in the soil-borne Fusarium wilt of cucumber plants induced uncontrolled water loss from damaged cells. FA plays a critical role in accelerating the development of Fusarium wilt in cucumber plants.

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Section: Discussionsupporting

confidence: 91%

Water balance altered in cucumber plants infected with Fusarium oxysporum f. sp. cucumerinum

Wang

Sun

et al. 2015

Sci Rep

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“…The different leaf temperature after inoculation may due to the different abscisic acid (ABA) content in response to the different N forms and supplies [46]. Generally, pathogen infection can induce stomatal closure [47,48], which is mediated by ABA content [7,49], thus decreasing the transpiration rate and increasing the leaf temperature [50,51]. …”

Section: Discussionmentioning

confidence: 99%

“…It was reported that FA is the critical causal agent of Fusarium wilt through leaf cell membrane injury [6,7]. Many studies have shown that FA disturbs the metabolism of infected plants and decreases the viability of plant cells, including the early hyperpolarization of the root membrane electrical potential [8]; alterations in membrane permeability [9]; an increase of electrolyte leakage [10,11]; the inhibition of defense enzymes and respiratory activity [12,13]; and a decrease in ATP levels and stomatal conductance [14,15], thus inhibiting plant growth and eventually leading to death [15,16,17].…”

Section: Introductionmentioning

confidence: 99%

“…We characterized the influence of N forms and supply levels on disease index dynamic changes, cucumber growth and leaf temperature changes to identify the parameters of the interaction that are affected by N supply. Moreover, our previous studies reported that FA plays a critical role in Fusarium wilt by causing leaf cell membrane injury [6,7]. Thus, FA was applied in the nutrient solution to further investigate the role of FA on Fusarium wilt in response to different N sources.…”

Section: Introductionmentioning

confidence: 99%

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Nitrate Increased Cucumber Tolerance to Fusarium Wilt by Regulating Fungal Toxin Production and Distribution

Zhou

Wang

Sun

et al. 2017

Toxins

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Cucumber Fusarium wilt, induced by Fusarium oxysporum f. sp. cucumerinum (FOC), causes severe losses in cucumber yield and quality. Nitrogen (N), as the most important mineral nutrient for plants, plays a critical role in plant–pathogen interactions. Hydroponic assays were conducted to investigate the effects of different N forms (NH4+ vs. NO3‒) and supply levels (low, 1 mM; high, 5 mM) on cucumber Fusarium wilt. The NO3‒-fed cucumber plants were more tolerant to Fusarium wilt compared with NH4+-fed plants, and accompanied by lower leaf temperature after FOC infection. The disease index decreased as the NO3‒ supply increased but increased with the NH4+ level supplied. Although the FOC grew better under high NO3− in vitro, FOC colonization and fusaric acid (FA) production decreased in cucumber plants under high NO3− supply, associated with lower leaf membrane injury. There was a positive correlation between the FA content and the FOC number or relative membrane injury. After the exogenous application of FA, less FA accumulated in the leaves under NO3− feeding, accompanied with a lower leaf membrane injury. In conclusion, higher NO3− supply protected cucumber plants against Fusarium wilt by suppressing FOC colonization and FA production in plants, and increasing the plant tolerance to FA.

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“…which can be used as a selecting agent for in vitro selection of resistant orchids (Mahlanza et al, 2013). FA is a suitable method used in in vitro selection because has low to moderate toxicity to human, plant and environment and can cause alteration in the morphology and growth in the infected plants per se (Wang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Preliminary study on the effects of fusaric acid treated protocorm-like bodies of Dendrobium hybrid against Fusarium proliferatum and Fusarium oxysporum

Sangu¹,

Nor²,

Zakaria³

et al. 2019

MJM

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Aims: Dendrobiums are majorly affected by Fusarium proliferatum and F. oxysporum. The aim of this research was to utilise the mycotoxin, fusaric acid (FA) on Dendrobium hybrid to produce cultivars that are resistant towards these fungi. Methodology and results: FA of concentrations 0.05, 0.10, 0.15 and 0.20 mM were transferred to sterilised halfstrength Murashige and Skoog (MS) medium and inoculated with four weeks old thin cell layer (TCL) of protocorm-like bodies (PLBs) for eight weeks. It was deduced that PLBs treated with 0.10 mM of FA resulted in highest survival and shoot regeneration rate but the survival and regeneration rate began to decline as the concentrations of FA were increased. Histology and scanning electron microscopy (SEM) observation showed prominent cell damage and stomatal closure in PLBs treated with FA. Direct amplification of minisatellite DNA (DAMD) markers showed polymorphism in the FA treated PLBs compared to the control PLBs. In the leaf bridge bioassay, plantlets treated with 0.05 mM of FA showed most resistance towards both fungal species. Conclusion, significance and impact of study: Therefore, this research is a preliminary screening study where the optimum concentration of FA was selected based on the reaction of treated TCL of PLBs towards these mutagens.

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Effect of fusaric acid on the leaf physiology of cucumber seedlings

Cited by 30 publications

References 50 publications

Water balance altered in cucumber plants infected with Fusarium oxysporum f. sp. cucumerinum

Water balance altered in cucumber plants infected with Fusarium oxysporum f. sp. cucumerinum

Nitrate Increased Cucumber Tolerance to Fusarium Wilt by Regulating Fungal Toxin Production and Distribution

Preliminary study on the effects of fusaric acid treated protocorm-like bodies of Dendrobium hybrid against Fusarium proliferatum and Fusarium oxysporum

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