Effect of fungicides on epiphytic yeasts associated with strawberry

Debode, Jane; Hemelrijck, Wendy Van; Creemers, P.; Maes, Martine

doi:10.1002/mbo3.85

Cited by 24 publications

(16 citation statements)

References 37 publications

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“…The copyright holder for this preprint this version posted December 7, 2020. ; https://doi.org/10.1101/2020.12.05.413260 doi: bioRxiv preprint influence pollinator visitation, which are perhaps more salient than microbes in mediating plantpollinator interactions in nature. Second, effects on only a few pollinator taxa have been tested to date (Good et al, 2014;Schaeffer et al, 2019;Vannette et al, 2013) Agrochemical disturbance from fungicide directly affected floral microbiome, consistent with the observations in other systems and/or plant organs (Bartlewicz et al, 2016;Debode, Van Hemelrijck, Creemers, & Maes, 2013;Gu et al, 2010;Schaeffer et al, 2017). Although oxytetracycline is among the most widely used bactericides (McManus et al, 2002;Vidaver, 2002), it caused non-significant reduction in bacterial α-diversity and mild changes in composition such as Hymenobacter, the common bacteria in strawberry flowers (Wei & Ashman, 2018).…”

Section: Discussionsupporting

confidence: 55%

Pollinators mediate floral microbial diversity and network under agrochemical disturbance

Wei¹,

Russell

Jarrett

et al. 2020

Preprint

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How pollinators mediate microbiome assembly in the anthosphere is a major unresolved question of theoretical and applied importance in the face of anthropogenic disturbance. We addressed this question by linking visitation of diverse pollinator functional groups (bees, wasps, flies, butterflies, beetles, true bugs and other taxa) to the key properties of floral microbiome (microbial α- and β-diversity and microbial network) under agrochemical disturbance, using a field experiment of bactericide and fungicide treatments on cultivated strawberries that differ in flower abundance. Structural equation modeling was used to link agrochemical disturbance and flower abundance to pollinator visitation to floral microbiome properties. Our results revealed that (1) pollinator visitation influenced the α- and β-diversity and network centrality of floral microbiome, with different pollinator functional groups affecting different microbiome properties; (2) flower abundance influenced floral microbiome both directly by governing the source pool of microbes and indirectly by enhancing pollinator visitation; and (3) agrochemical disturbance affected floral microbiome primarily directly by fungicide, and less so indirectly via pollinator visitation. These findings improve the mechanistic understanding of floral microbiome assembly, and may be generalizable to many other plants that are visited by diverse insect pollinators in natural and managed ecosystems.

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Section: Discussionsupporting

confidence: 55%

Pollinators mediate floral microbial diversity and network under agrochemical disturbance

Wei¹,

Russell

Jarrett

et al. 2020

Preprint

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“…Harvested fruits were infected with B. cinerea isolate 895 (Debode et al, 2013 ) based on the method of Bhaskara Reddy et al ( 2000 ). Briefly, individual ripe strawberry fruits were inoculated with 20 μl conidial suspension (2 × 10 5 conidia ml −1 ) and incubated at 11°C in humid conditions.…”

Section: Methodsmentioning

confidence: 99%

Trichoderma-Inoculated Miscanthus Straw Can Replace Peat in Strawberry Cultivation, with Beneficial Effects on Disease Control

Debode¹,

Tender

Cremelie³

et al. 2018

Front. Plant Sci.

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Peat based growing media are not ecologically sustainable and often fail to support biological control. Miscanthus straw was (1) tested to partially replace peat; and (2) pre-colonized with a Trichoderma strain to increase the biological control capacity of the growing media. In two strawberry pot trials (denoted as experiment I & II), extruded and non-extruded miscanthus straw, with or without pre-colonization with T. harzianum T22, was used to partially (20% v/v) replace peat. We tested the performance of each mixture by monitoring strawberry plant development, nutrient content in the leaves and growing media, sensitivity of the fruit to the fungal pathogen Botrytis cinerea, rhizosphere community and strawberry defense responses. N immobilization by miscanthus straw reduced strawberry growth and yield in experiment II but not in I. The pre-colonization of the straw with Trichoderma increased the post-harvest disease suppressiveness against B. cinerea and changed the rhizosphere fungal microbiome in both experiments. In addition, defense-related genes were induced in experiment II. The use of miscanthus straw in growing media will reduce the demand for peat and close resource loops. Successful pre-colonization of this straw with biological control fungi will optimize crop cultivation, requiring fewer pesticide applications, which will benefit the environment and human health.

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“…Plant leaves were inoculated using the method of Meller Harel et al (2012). Briefly, the B. cinerea isolate 895 (Debode et al, 2013) was cultured on Potato Dextrose Agar (PDA) at 20°C for 4 days. Agar discs (4 mm) containing pathogen mycelium and conidiophores were cut out from the colony edge and placed, mycelium side down, on the surface of three young fully expanded strawberry leaves per plant, with one disc per leaflet.…”

Section: Methodsmentioning

confidence: 99%

Dynamics in the Strawberry Rhizosphere Microbiome in Response to Biochar and Botrytis cinerea Leaf Infection

Tender

Haegeman²,

Vandecasteele³

et al. 2016

Front. Microbiol.

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Adding biochar, the solid coproduct of biofuel production, to peat can enhance strawberry growth, and disease resistance against the airborne fungal pathogen Botrytis cinerea. Additionally, biochar can induce shifts in the strawberry rhizosphere microbiome. However, the moment that this biochar-mediated shift occurs in the rhizosphere is not known. Further, the effect of an above-ground infection on the strawberry rhizosphere microbiome is unknown. In the present study we established two experiments in which strawberry transplants (cv. Elsanta) were planted either in peat or in peat amended with 3% biochar. First, we established a time course experiment to measure the effect of biochar on the rhizosphere bacterial and fungal communities over time. In a second experiment, we inoculated the strawberry leaves with B. cinerea, and studied the impact of the infection on the rhizosphere bacterial community. The fungal rhizosphere community was stabilized after 1 week, except for the upcoming Auriculariales, whereas the bacterial community shifted till 6 weeks. An effect of the addition of biochar to the peat on the rhizosphere microbiome was solely measured for the bacterial community from week 6 of plant growth onwards. When scoring the plant development, biochar addition was associated with enhanced root formation, fruit production, and postharvest resistance of the fruits against B. cinerea. We hypothesize that the bacterial rhizosphere microbiome, but also biochar-mediated changes in chemical substrate composition could be involved in these events. Infection of the strawberry leaves with B. cinerea induced shifts in the bacterial rhizosphere community, with an increased bacterial richness. This disease-induced effect was not observed in the rhizospheres of the B. cinerea-infected plants grown in the biochar-amended peat. The results show that an above-ground infection has its effect on the strawberry rhizosphere microbiome, changing the bacterial interactions in the root-substrate interface. This infection effect on the bacterial rhizosphere microbiome seems to be comparable to, but less pronounced than the effect of biochar-addition to the peat. The biological meaning of these observations needs further research, but this study indicates that biochar and an above-ground pathogen attack help the plant to recruit rhizosphere microbes that may aid them in their plant growth and health.

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Effect of fungicides on epiphytic yeasts associated with strawberry

Cited by 24 publications

References 37 publications

Pollinators mediate floral microbial diversity and network under agrochemical disturbance

Pollinators mediate floral microbial diversity and network under agrochemical disturbance

Trichoderma-Inoculated Miscanthus Straw Can Replace Peat in Strawberry Cultivation, with Beneficial Effects on Disease Control

Dynamics in the Strawberry Rhizosphere Microbiome in Response to Biochar and Botrytis cinerea Leaf Infection

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