Effect of fullerenol C60(OH)24 on lipid peroxidation of kidneys, testes and lungs in rats treated with doxorubicine

Abstract

The aim of this study is to investigate the protective effect of fullerenol C₆₀(OH)₂₄ in various doses, on lipid peroxidation of rat's kidneys, testes and lungs after application of doxorubicin. The experiment was performed on healthy male Wistar rats. The animals were randomly divided into five experimental groups and treated with saline (0.9 % NaCl i.v.), doxorubicin alone (10 mg/kg i.v.), combination of doxorubicin/fullerenol (50 and 100 mg/kg fullerenol, respectively, 30 min before the introduction of doxorubicin) and fullerenol alone (100 mg/kg), respectively. Animals were killed on the 2nd and 14th day after treatment. Products of lipid peroxidation and thiobarbituric acid are determined spectrophotometrically from the crude homogenate fraction of the kidney, testis and lung tissues of the rats. Fullerenol, applied as a pre-treatment of doxorubicin, significantly reduced or completely prevented the appearance of doxorubicin toxicity in kidneys and testes, in both tested doses. A dose of 100 mg/kg i.p. exhibited a better protective effect. When fullerenol was applied alone, at a dose of 100 mg/kg i.p, it did not significantly affect the intensity of lipid peroxidation in all tested organs.

Antioxidative nanofullerol prevents intervertebral disk degeneration

“…Among numerous reported fullerene derivatives, nanofullerol is the most extensively studied, and has been implicated as a powerful antioxidant in the biomedical field. [48][49][50][51][52][53][54][55][56] Therefore, fullerol was selected in our present study.…”

Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells

“…31 Our present data showed that fullerol served as an antioxidant in human ADSCs without cytotoxicity at doses up to 10 µM (Figures 1 and 2), and this is in agreement with a majority of previous reports with regards to other tissues and cell types. [17][18][19]28,[32][33][34][35][36][37][38][39] It has been shown that ROS inhibit osteogenesis and bone formation. Mody et al 40 reported that three oxidants, hydrogen peroxide (H 2 O 2 ), xanthine, and xanthine oxidase, and minimally oxidized low-density lipoproteins inhibited osteoblastic differentiation of MC3T3-E1 mouse bone preosteoblast cell line and M2-10B4 mouse marrow stromal cell line, while increasing intracellular oxidative stress.…”

Bioimpact of Carbon Nanomaterials

“…(higher dose) in combined therapy exhibited a better protective effect. On the other hand, fullerenol applied alone at the same dose did not show signifi cant infl uence on lipid peroxidation in all tested organs [117].…”

“…The effect of fullerenol C 60 (OH) 24 on lipid peroxidation in the kidneys, testes and lungs of rats treated with doxorubicin has been recently published by Vapa et al [117]. The experiment was conducted with healthy male Wistar rats divided into fi ve investigation groups.…”