Effect of free and encapsulated Pseudomonas putida CC-FR2-4 and Bacillus subtilis CC-pg104 on plant growth under gnotobiotic conditions

Rekha, P. D.; Lai, Wei-An; Arun, A. B.; Young, Chiu-Chung

doi:10.1016/j.biortech.2006.01.009

Cited by 95 publications

(46 citation statements)

References 22 publications

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“…A similar trend of solubilization was observed with ER phosphate also. This may be attributed to the survival of bacterial cells from newly introduced environment and thereby reducing cell loss in immobilized form (Rekha et al, 2007). Immobilization provides protective environment with nutrient source (Saxena, 2011).…”

Section: Resultsmentioning

confidence: 99%

Application of Immobilization Technology in Solubilization of Rock Phosphate

Walpola¹,

Kim²,

Jeon³

et al. 2014

Korean Journal of Soil Science and Fertilizer

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Phosphates solubilizing bacterial strains belong to Pantoea, Burkholderia and Enterobacter were isolated and employed in assessing their solubilization ability of Ca phosphate and ER phosphate (Eppawala Rock Phosphate). Among the bacterial strains used, PSB-13 (Pantoea rodasii) showed higher Ca-phosphate solubilization (1100 µg ml -1 ) as well as rock phosphate solubilization (168 µg ml -1 ). The strain was then immobilized in agar to further assess its phosphate solubilization ability. According to the results, agar encapsulated strain solubilized 0.3%, 7.31%, 20.24%, and 20.62% more Ca-phosphate and 11.53%, 15.29%, 28.48%, 36.55% (respectively in 4 cycles) more ER-phosphate than free cells. The reuse efficiency of agar entrapped bacterial cells for Ca-phosphate and ER-phosphate solubilization was greater than that by freely suspended bacterial cells. In conclusion, immobilization could enhance the phosphate solubilization capacity of the strains and thus could be used effectively in enhancing solubilization of ER phosphate.Key words: Pantoea rodasii, Immobilization, Phosphate solubilization, Eppawala rock phosphate

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Section: Resultsmentioning

confidence: 99%

Application of Immobilization Technology in Solubilization of Rock Phosphate

Walpola¹,

Kim²,

Jeon³

et al. 2014

Korean Journal of Soil Science and Fertilizer

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“…In this study we have opted to use calcium alginate hydrogel that is gelled by ionic bonds and swells and dissolves in a solution containing phosphates, a chelating agent present in the soil [6]. Although several biomaterials are available, the alginate is preferred because the capsules are formed instantaneously by one-step gelling with cell loss reduced to a minimum [25] and the microbial cell density could be maintained as high as high as 10 10 -10 11 CFUg -1 of capsule [8]. In our study, we achieved a concentration of 8.08 Log CFU.g -1 of bacteria in freeze-dried microcapsules and a survival rate of 14%; a concentration close to what was achieved under similar conditions (8.28 Log CFU.g -1 ) with Lactoacillus casei [14].…”

Section: Survival Of B Subtilis B26mentioning

confidence: 99%

“…Encapsulation of living cells in polymeric gels is a well-established technology suitable for a wide range of applications [4,7]. Several studies thus far have used alginate matrix as the encapsulating material since it forms microbeads instantaneously in the presence of polyvalent cations such as calcium [6,8]. Alginate beads have several positive attributes: (i) they are capable of entrapping a sufficient number of bacteria [9]; (ii) they protect the cells by providing a pre-defined and constant microenvironment thus allowing the cells to survive and maintain metabolic activity for extended period of time, and (iii) they provide a controlled release of microroganisms as well as serve as energy source for the microorganisms [10].…”

Section: Introductionmentioning

confidence: 99%

Pea Protein Alginate Encapsulated Bacillus subtilis B26, a Plant Biostimulant, Provides Controlled Release and Increased Storage Survival

Gagné-Bourque¹,

Xu²

2015

J Fertil Pestic

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Plant growth promoting bacteria (PGPB) represent a wide variety of soil and endophytic bacteria that have the ability to promote growth or to protect against stress of its host plant. Encapsulation of PGPB, using vegetable proteins instead of animal or petroleum-derived polymers, is a new technology to crop production and protection. Pea protein isolates (PPI) alginate capsules were synthesized and used for the protection and delivery of Bacillus subtilis B26 as plant inoculum for agricultural applications. The capsules provided a protective site to B26 strain allowing good survival, between 45 to 50%, after 112 days of incubation at different temperatures. The loaded microcapsules sustained a large population of bacteria, up to 8.3 LogCFUg -1 of soil after 3 weeks post application. The soil concentration stabilized to 7.3 Log CFUg -1 after 8 weeks post application. Capsules loaded with B26, once incorporated in the soil, successfully colonized test plants, and cell numbers inside plant tissues were sustained at 3.5 and 3.3 Log 7.3 CFUg -1 in the root and the shoot respectively. These results indicate that PPIalginate technology represents a good choice for commercial application of B. subtilis B26 in agriculture.Citation: Gagné-Bourque F, Meng Xu, Dumont MJ, Jabaji S (2015) Materials and Methods Maintenance and preparation of B. subtilis B26 inoculumThe B.subtilis strain B26, previously isolated from switchgrass and fully characterized [15], was maintained on Luria Broth (LB) (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl) with glycerol (25% final volume) and stored at-80°C. B. subtilis B26 was revived on LBA (1.5% Agar) plates. The inoculum was prepared by placing a single colony of B. subtilis B26 in 250 mL of LB and incubated for 18 h at 37°C until an OD600 of 0.7, representing colony forming units (CFU) of 3.38 × 10 8 / mL was reached on a shaker at 250 rpm to the mid-log phase, pelleted by centrifugation, washed and suspended in sterile distilled water. Preparation of alginate capsules and microencapsulationPea protein isolates (PPI) (Propulse N™) containing 81.73% protein, <10.3% sugars, <0.7% starch, <3.4% moisture, <0.5% fat, <4.0% ash was obtained from Nutri-Pea Ltd. (Portage la Prairie, MB, Canada). Alginic acid sodium salt with low viscosity (viscosity of 1% aq. solution: <300cps) was purchased from MP biomedicals (Solon, OH, USA). Calcium chloride (CaCl 2 ) dehydrate, hydrochloric acid (HCl) and dimethyl sulfoxide (DMSO) were purchased from Fisher Scientific (Fair Lawn, NJ). Sodium hydroxide was purchased from EMD (Damstadt, Germany). Tryptone, Yeast Extract, NaCl, and Agar were purchased from Difco (Franklin Lakes, NJ, USA). Ammonium sulphate, Potassium phosphate dibasic trihydrate, monopotassium phosphate, trisodium citrate and magnesium sulfate heptahydrate were purchased from Sigma-Aldrich (Co., St. Louis, MO). Glycerol was purchased from ThermoFisher Scientific (Waltham, MA). The qPCR SYBRII master mix and the ROX were purchased from Agilent Technologies (Morrisville, NC, USA) while B...

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“…비록 대상 식물 종과 환 경조건이 다르지만 alginate에 고정한 Pseudomonas fluorescens BAM-4와 Burkholderia cepacia BAM-12가 밀의 shoot 길이 생 장을 비접종 대조군보다30-40% 정도 증가시킨 결과보다 우수 하였으며 (Minaxi, 2011), 상추(Lectuca sativa)를 대상으로 humic acid를 첨가한 alginate에 Pseudomonas putida CC-FR2-4를 고 정화하여 접종했을 때 현탁액 접종보다 shoot와 뿌리 길이가 5-10% 정도로 생장이 증가한 결과와 유사하였다 (Rekha et al, 2007). 현탁액 접종에 비해 고정화 균주 접종의 식물생장 촉진효 과가 높은 것은 고정화 균주가 한꺼번에 방출되지 않고 지속적 으로 식물 근권에 공급되며 안정적으로 식물생장 촉진활성을 나 타내기 때문인 것으로 추정된다 (Vassilev et al, 2001;Rekha et al, 2007). 한편 균주 비함유 control alginate bead 실험군은 비 접종 대조군에 비해 토마토의 shoot 길이, 뿌리길이, 습윤중량과 건조중량이 각각 3.9, 2.6, 13.7과 17.7% 증가하였으나 유의성은 나타나지 않았다.…”

Section: 세균군집 Dna 추출 및 증폭unclassified

“…이제까지 고정화 PGPR 균주에 의한 식물생장촉진 연구는 많 았지만 균주의 근권 잔류에 대해서는 조사하지 않거나 (Rekha et al, 2007;Schoebitz et al, 2014) 고정화 담체에서 방출되는 생 균수만을 측정한 경우가 대부분이었으며 (Bashan et al, 2002;Minaxi, 2011 …”

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Growth Promotion of Tomato by Application of Immobilized Arthrobacter woluwensis ED in Alginate Beads

Kwon¹,

Song

2014

The Korean Journal of Microbiology

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In order to increase the persistence of plant growth promoting rhizobacteria (PGPR) in rhizpsphere soil, the growth of tomato was examined after the application of Arthrobacter woluwensis ED immobilized in alginate bead, which was known as PGPR. When tomato seedlings were treated with A. woluwensis ED of 1 × 10 6 cells g soil -1 and incubated for 30 days in a plant growth chamber, the shoot length, root length, fresh weight and dry weight of the grown tomato plants treated with the suspended inoculants significantly increased by 36.2, 59, 51.1, and 37.5%, respectively compared to those of the uninoculated control. The treatment of the immobilized bacteria increased those by 42, 67.4, 62.5, and 60.4%, respectively compared to those of the uninoculated control. Therefore, the enhancement of tomato growth by the treatment of the immobilized bacteria was higher than those by the suspended inoculants. The effects of the inoculation on indigenous bacterial community and the fate of the inoculated bacteria were monitored by denaturing gradient gel electrophoresis analysis. The DNA band intensity of A. woluwensis ED in the tomato rhizosphere treated with the suspended inoculants continuously decreased after the inoculation, but the band intensity in the tomato rhizosphere soils treated with the immobilized inoculants showed the maximum at 1 week after inoculation and the decreasing rate was less than that of the suspended inoculants, which indicated the longer maintenance of the immobilized bacteria at rhizosphere soils. Therefore, encapsulation of PGPR in alginate beads may be more effective than liquid inoculant for the plant growth promotion and survival of PGPR at plant rhizosphere.

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Effect of free and encapsulated Pseudomonas putida CC-FR2-4 and Bacillus subtilis CC-pg104 on plant growth under gnotobiotic conditions

Cited by 95 publications

References 22 publications

Application of Immobilization Technology in Solubilization of Rock Phosphate

Application of Immobilization Technology in Solubilization of Rock Phosphate

Pea Protein Alginate Encapsulated Bacillus subtilis B26, a Plant Biostimulant, Provides Controlled Release and Increased Storage Survival

Growth Promotion of Tomato by Application of Immobilized Arthrobacter woluwensis ED in Alginate Beads

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