Effect of Forage Sterilization Method on Rate and Extent of Fiber Degradation by Ruminal Bacteria

Lancaster, Carmel; Patterson, J. A.

doi:10.2527/jas1988.6671800x

Cited by 7 publications

(2 citation statements)

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“…As suggested by Osborne and Dehority (1989) and demonstrated by Fondevila and Dehority (1994), the sequential addition of microorganisms after inactivation of the first organism can be a very useful procedure for studies on interactions of ruminal bacteria. Although several studies in the literature indicate some solubilization of carbohydrate as an effect of autoclaving forage in liquid media (Morris and Van Gylswyk, 1980;Graham et al, 1985;Lancaster and Patterson, 1988), we did not find any effect of a second autoclaving on the extent of forage cellulose digestion (present study) or hemicellulose digestion (Fondevila and Dehority, 1994).…”

Section: Discussioncontrasting

confidence: 94%

Interactions between Fibrobacter succinogenes, Prevotella ruminicola, and Ruminococcus flavefaciens in the digestion of cellulose from forages.

Fondevila

Dehority

1996

Journal of Animal Science

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The synergistic and inhibitory interactions observed between Fibrobacter succinogenes A3c, Prevotella ruminicola H2b, and Ruminococcus flavefaciens B34b in the digestion of forage cellulose were studied in detail. Orchardgrass and alfalfa hays, both at two maturity stages, were used as substrates. Sequential inoculation procedures were developed whereby a second inoculation was made after the initial fermentation was killed. Total cellulose digestion from sequential addition of the organisms was then compared to values obtained in simultaneous co-culture. When the noncellulolytic P. ruminicola was co-cultured with either of the two cellulolytic species (F. succinogenes or R. flavefaciens) forage cellulose digestion numerically increased over that of the cellulolytic species alone. In contrast, decreases from co-culture values were noted with sequential addition of the organisms. When F. succinogenes and R. flavefaciens were co-cultured, cellulose digestion was reduced compared to F. succinogenes alone. However, no such reduction was observed when the organisms were added sequentially. Further experiments indicated that this inhibitory activity is only produced when the organisms are co-cultured and is stable to autoclaving at 121 degrees C for 20 min. Inhibition of this type could be the result of bacterocin production by one of the organisms; however, most bacterocins are destroyed by autoclaving.

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Section: Discussioncontrasting

confidence: 94%

Interactions between Fibrobacter succinogenes, Prevotella ruminicola, and Ruminococcus flavefaciens in the digestion of cellulose from forages.

Fondevila

Dehority

1996

Journal of Animal Science

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“…In order to remove any potential artifacts associated with heating (weakening of the lucerne cell walls or slide-mounted lucerne sections, or production of fermentation inhibitors), the lucerne was not sterilized prior to inoculation. Lancaster & Patterson (1988) reported that autoclaving and boiling of forages resulted in reduced rate and extent of in vitro ruminal degradation. Instead, forages were air-dried at 50 o C to inactivate most mesophilic non-sporeforming anaerobic vegetative cells, and following addition of culture medium a large inoculum of the desired test culture was added.…”

Section: Componentmentioning

confidence: 99%

Degradation of lucerne stem cell walls by five rumen bacterial species

Jung

Engels

Weimer

2004

NJAS: Wageningen Journal of Life Sciences

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The rumen bacterial strains Butyrivibrio fibrisolvens H17c, Fibrobacter succinogenes S85, Lachnospira multiparus 40, Ruminococcus albus 7 and R. flavefaciens FD-1 were compared individually and as a five-species mixture with a rumen inoculum for their ability to degrade lucerne (Medicago sativa L.) stem cell wall polysaccharides. Two maturity stages of lucerne (bud and full flower) were utilized as substrates and incubation periods of 24 and 96 h were employed. Lucerne stem substrates and fermentation residues were analysed for cell wall content and composition. Degradation of total cell wall polysaccharides and all polysaccharide fractions was greater for immature than for mature lucerne stems. The 96-h incubation resulted in significantly more degradation of all cell wall polysaccharides than did the 24-h incubation. The R. albus culture was able to degrade all cell wall polysaccharide fractions as well or better than any other culture, including the rumen inoculum. Co-culture of R. albus with the four other ruminal species did not improve degradation of any wall polysaccharides compared with R. albus alone. Microscopic examination indicated that R. albus and the five-species mixture appeared to degrade lucerne tissues in thin sections to almost the same extent as did rumen fluid.

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Management, Husbandry, and Colony Health

Gresham

Haines

2012

The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents

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Effect of Forage Sterilization Method on Rate and Extent of Fiber Degradation by Ruminal Bacteria

Cited by 7 publications

References 0 publications

Interactions between Fibrobacter succinogenes, Prevotella ruminicola, and Ruminococcus flavefaciens in the digestion of cellulose from forages.

Interactions between Fibrobacter succinogenes, Prevotella ruminicola, and Ruminococcus flavefaciens in the digestion of cellulose from forages.

Degradation of lucerne stem cell walls by five rumen bacterial species

Management, Husbandry, and Colony Health

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