Interactions between Fibrobacter succinogenes, Prevotella ruminicola, and Ruminococcus flavefaciens in the digestion of cellulose from forages.

Fondevila, M.; Dehority, Burk A.

doi:10.2527/1996.743678x

Cited by 75 publications

(67 citation statements)

References 34 publications

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“…While cellulolytic species may compete directly for cellulose (104,192,193,499,616), both cellulolytic and noncellulolytic species can compete for cellodextrin products of cellulose hydrolysis, in cross-feeding of nutrients, and in production of inhibitory compounds. A number of examples of such interactions have been demonstrated in defined mixed cultures (Table 2).…”

Section: Ecological Aspects Of Cellulose-degrading Communitiesmentioning

confidence: 99%

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

4,014

2,868

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Fundamental features of microbial cellulose utilization are examined at successively higher levels of aggregation encompassing the structure and composition of cellulosic biomass, taxonomic diversity, cellulase enzyme systems, molecular biology of cellulase enzymes, physiology of cellulolytic microorganisms, ecological aspects of cellulase-degrading communities, and rate-limiting factors in nature. The methodological basis for studying microbial cellulose utilization is considered relative to quantification of cells and enzymes in the presence of solid substrates as well as apparatus and analysis for cellulose-grown continuous cultures. Quantitative description of cellulose hydrolysis is addressed with respect to adsorption of cellulase enzymes, rates of enzymatic hydrolysis, bioenergetics of microbial cellulose utilization, kinetics of microbial cellulose utilization, and contrasting features compared to soluble substrate kinetics. A biological perspective on processing cellulosic biomass is presented, including features of pretreated substrates and alternative process configurations. Organism development is considered for “consolidated bioprocessing” (CBP), in which the production of cellulolytic enzymes, hydrolysis of biomass, and fermentation of resulting sugars to desired products occur in one step. Two organism development strategies for CBP are examined: (i) improve product yield and tolerance in microorganisms able to utilize cellulose, or (ii) express a heterologous system for cellulose hydrolysis and utilization in microorganisms that exhibit high product yield and tolerance. A concluding discussion identifies unresolved issues pertaining to microbial cellulose utilization, suggests approaches by which such issues might be resolved, and contrasts a microbially oriented cellulose hydrolysis paradigm to the more conventional enzymatically oriented paradigm in both fundamental and applied contexts

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Section: Ecological Aspects Of Cellulose-degrading Communitiesmentioning

confidence: 99%

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

4,014

2,868

View full text Add to dashboard Cite

show abstract

“…Several reports have also described interactions between two or among three cellulolytic species of ruminal bacteria (Odenyo et al, 1994a, b ;Fondevila & Dehority, 1996 ;Mosoni et al, 1997 ;. Most of these studies have been performed under batch culture conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Their data indicated that R. albus 8 generally out-competed R. flavefaciens FD-1, due to production by the former of a bacteriocin-like substance. Fondevila & Dehority (1996) presented evidence that cellulose digestion was reduced when strains of F. succinogenes A3c and R. flavefaciens B34b were grown together in batch culture and suggested that an inhibitor was produced by one of the two species. Mosoni et al (1997) reported that R. flavefaciens FD-1 became detached from cellulose in the presence of R. albus 20.…”

Section: Introductionmentioning

confidence: 99%

Competition among three predominant ruminal cellulolytic bacteria in the absence or presence of non-cellulolytic bacteria

Chen

Weimer

2001

Microbiology

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Competition among three species of ruminal cellulolytic bacteria -Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD-1 and Ruminococcus albus 7 -was studied in the presence or absence of the non-cellulolytic ruminal bacteria Selenomonas ruminantium or Streptococcus bovis. Co-cultures were grown under either batch or continuous conditions and populations were estimated using species-specific oligonucleotide probes to 16S rRNA. The three cellulolytic species co-existed in cellobiose batch co-culture, but inclusion of either Sel. ruminantium or Str. bovis yielded nearly a monoculture of the noncellulolytic competitor. In cellobiose chemostats, R. albus completely dominated the triculture, but R. flavefaciens became predominant over F. succinogenes and R. albus when Sel. ruminantium was co-inoculated into the chemostats. Similar effects on competition were observed in the presence of Str. bovis at a lower (0021 h N1 ), but not at a higher (0045 h N1 ) dilution rate. In cellulose batch co-cultures, R. albus was more abundant than both F. succinogenes and R. flavefaciens, regardless of the presence of the noncellulolytic species. Co-existence among the three cellulolytic species was observed in almost all cellulose chemostats, but Sel. ruminantium altered the relative proportions of the cellulolytic species. R. albus and R. flavefaciens were found to produce inhibitors that suppressed growth of R. flavefaciens and F. succinogenes, respectively. These data indicate that interactions among cellulolytic bacteria, while complex, can be modified further by non-cellulolytic species.

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“…Cellulose is digested in the rumen (Michalet-Doreau et al 2002). The ability to degrade cellulose depends mainly on the type of forage, crop maturity, and the members of the cellulolytic bacterial communities (Fondevila and Dehority 1996). To ensure maintenance and growth of cellulolytic bacteria, optimal ruminal conditions are required.…”

Section: Cellulose-degrading Bacteriamentioning

confidence: 99%

Rumen microorganisms and fermentation

et al. 2014

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RESUMENEl rumen es un ecosistema complejo donde los nutrientes consumidos por los rumiantes son digeridos mediante un proceso de fermentación realizado por los microorganismos ruminales (bacterias, protozoos y hongos). Dichos microorganismos están en simbiosis, debido a su capacidad de adaptación e interacción, y mientras el rumiante proporciona el ambiente necesario para su establecimiento estos proporcionan energía al animal, la que proviene de los productos finales de la fermentación. Dentro del rumen, los microorganismos coexisten en un entorno reducido y a un pH cercano a la neutralidad. Estos microorganismos fermentan los sustratos presentes en la dieta del rumiante (azúcares, proteínas y lípidos). Sin embargo, el proceso de fermentación no es 100% eficaz, ya que durante la fermentación existen pérdidas de energía, principalmente en forma de gas metano (CH 4 ), el que representa un problema medioambiental, ya que es un gas de efecto invernadero. Por consiguiente, para mejorar la eficiencia de los sistemas de producción de rumiantes se han establecido estrategias nutricionales que tienen como objetivo manipular la fermentación ruminal mediante el uso de aditivos en la dieta, como monensina, sebo, tampones, compuestos de nitrógeno, probióticos, etc. Estos aditivos permiten cambiar el proceso de fermentación y mejorar la eficiencia animal, además disminuyen la pérdida de energía. El objetivo de este trabajo es revisar los procesos fermentativos que tienen lugar en el rumen y aplicar los fundamentos de estos en el desarrollo de nuevas estrategias nutricionales que pudieran ayudar a mejorar los procesos de digestión, de manera que se alcance una máxima producción.Palabras clave: aditivos, microorganismos ruminales, simbiosis. SUMMARYThe rumen consists of a complex ecosystem where nutrients consumed by ruminants are digested by fermentation process, which is executed by diverse microorganisms such as bacteria, protozoa, and fungi. A symbiotic relationship is found among different groups of microorganisms due to the diverse nature of these microbial species and their adaptability and interactions also coexist. The ruminant provides the necessary environment for the establishment of such microorganisms, while the microorganisms obtain energy from the host animal from microbial fermentation end products. Within the ruminal ecosystem, the microorganisms coexist in a reduced environment and pH remains close to neutral. Rumen microorganisms are involved in the fermentation of substrates contained in thedietof the animals (carbohydrates, proteins and lipids). However, the fermentation process is not 100% effective because there are energy losses mainly in the form of methane gas (CH 4 ), which is a problem for the environment since it is a greenhouse gas. In order to improve the efficiency of ruminant production systems, nutritional strategies that aim to manipulate ruminal fermentation using additives in the diet such as monensin, tallow, buffers, nitrogen compounds, probiotics, and others have been used. These additi...

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Interactions between Fibrobacter succinogenes, Prevotella ruminicola, and Ruminococcus flavefaciens in the digestion of cellulose from forages.

Cited by 75 publications

References 34 publications

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Competition among three predominant ruminal cellulolytic bacteria in the absence or presence of non-cellulolytic bacteria

Rumen microorganisms and fermentation

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