Effect of folic acid on human trophoblast health and function in vitro

Ahmed, Tarem; Fellus, Ilan; Gaudet, Jeremiah; MacFarlane, Amanda J.; Fontaine-Bisson, Bénédicte; Bainbridge, Shannon

doi:10.1016/j.placenta.2015.11.012

Cited by 30 publications

(18 citation statements)

References 42 publications

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“…Folate alters cell proliferation, and specific inhibition of folate cycle has been shown to reduce the cell proliferation of the embryo (O'Neill, 1998). Meanwhile, human placenta cell line cultures exposed to high FA reduced cell viability and increased proliferation rate (Ahmed et al, 2016). Concordant with that, in this project, high FA diet increased the mitotic index (cell undergoing division) in the P.I group.…”

Section: Discussionsupporting

confidence: 74%

The Effect of Folic Acid Supplementation in the Ovary and Upon Embryo Development

et al. 2019

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Faculty of Natural and Environmental Sciences School of Biological Sciences Thesis for the degree of Doctor of Philosophy The Effect of Folic Acid Supplementation in the Ovary and Upon Embryo Development by Reyna Stephanie Penailillo Escarate Altered maternal nutrition around conception can affect oocyte and embryo development which influences later-life health outcomes. Low folate is related to poor reproductive outcomes. Folic acid (FA) supplementation and fortification have been effective strategies to avoid NTD. On the other hand, the FA levels in women of reproductive age are increased more than recommended, with unknown consequences. This project aims to determine the effect of high FA diet on the

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Section: Discussionsupporting

confidence: 74%

The Effect of Folic Acid Supplementation in the Ovary and Upon Embryo Development

et al. 2019

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“…Interestingly, tumors in the two negative control groups tend to grow faster compared to PBS control; however, statistical significance was not observed. One possible reason might be the overall slower tumor growth rate with folate deficient diet was rescued by certain growth factors contained in GDENs 45 , 46 . Follow-up studies on the effect of blank GDENs may be valuable since ginger has been widely used in food consumption as well as herb and nutrition supplement, and several studies also reported that ginger derived exosome-like nanoparticles had treatment effect in different disease models by oral administration 17 , 47 .…”

Section: Discussionmentioning

confidence: 99%

Arrowtail RNA for Ligand Display on Ginger Exosome-like Nanovesicles to Systemic Deliver siRNA for Cancer Suppression

Wang

Yin

et al. 2018

Sci Rep

119

102

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Exosomes have shown increasing potential as delivery vesicles for therapy, but challenges like cost/yield, drug payload, and targeting specificity still exist. Plant derived exosome-like nanoparticles have been reported as a promising substitution and exhibit biocompatibility through oral, intranasal administration; however, systemic delivery of siRNA by exosome-like nanoparticles directly isolated from plants has not been reported. Recently, we reported the control of RNA orientation to decorate human derived exosome with cell targeting ligands for specific delivery of siRNA to tumors. Here, we expand to the application of arrowtail RNA nanoparticles for displaying ligands on ginger derived exosome-like nanovesicles (GDENs) for siRNA delivery and tumor inhibition through IV administration. Cushion ultracentrifugation coupled with equilibrium density gradient ultracentrifugation were used for purifying GDENs that displayed size, density, and morphology similar to human derived exosomes. Folic acid (FA), as a ligand, was displayed on the surface of GDENs for targeted delivery of survivin siRNA to KB cancer models. In vitro gene knockdown efficacy by FA-3WJ/GDENs/siRNA complex was comparable to transfection. We observed inhibition of tumor growth on a xenograft model by intravenous administration, which reveals the potential of GDENs as an economic delivery system for siRNA.

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“…Seven studies have analyzed the effect of a wide range of FA concentrations on bone tissue formation in vitro in different cell types: bone marrow mesenchymal cells [9], mouse palate [34], cells of the mouse embryonic neural crest [33], osteoblasts [35], osteoclasts [36], human placenta cell lines [37], and directly on chicken embryos [38] (Table 3). Most studies supplement vitamins in cell cultures through the culture medium; however, Santos et al exposed cell cultures to hydroxyapatite nanoparticles loaded with FA [9].…”

Section: Folic Acid and Its Effect On Cell Culturesmentioning

confidence: 99%

Effect of folic acid on animal models, cell cultures, and human oral clefts: a literature review

Bendahan

Escobar

Castellanos

et al. 2020

Egypt J Med Hum Genet

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Background Folate is a naturally occurring, water-soluble B vitamin. The synthetic form of this compound is folic acid (FA), the deficiency of which is linked to neural tube disorders (NTD), which can be prevented by consuming it before, or during the early months of, pregnancy. However, the effect of FA on oral cleft formation remains controversial. The aim of the present study was to review the evidence concerning the effect of FA on the formation of cleft lip and palate (CLP) in both animals and humans, as well as its impact on different cell types. A search was conducted on various databases, including MEDLINE, EMBASE, and Central, for articles published until January 2020. Main body Current systematic reviews indicate that FA, alone or in combination with other vitamins, prevents NTD; however, there is no consensus on whether its consumption can prevent CLP formation. Conversely, the protective effect of FA on palatal cleft (CP) induction has been inferred from animal models; additionally, in vitro studies enumerate a cell-type and dose-dependent effect of FA on cell viability, proliferation, and differentiation, hence bolstering evidence from epidemiological studies. Conclusions Meta-analysis, animal models, and in vitro studies demonstrated the protective effect of FA against isolated CP; however, the heterogeneity of treatment protocols, doses, and FA administration method, as well as the different cell types used in in vitro studies, does not conclusively establish whether FA prevents CLP formation.

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Effect of folic acid on human trophoblast health and function in vitro

Cited by 30 publications

References 42 publications

The Effect of Folic Acid Supplementation in the Ovary and Upon Embryo Development

The Effect of Folic Acid Supplementation in the Ovary and Upon Embryo Development

Arrowtail RNA for Ligand Display on Ginger Exosome-like Nanovesicles to Systemic Deliver siRNA for Cancer Suppression

Effect of folic acid on animal models, cell cultures, and human oral clefts: a literature review

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