Effect of Fluoride in Drinking Water on Fecal Microbial Community in Rats

Zhong, Nan; Ma, Yongzheng; Meng, Xinyue; Sowanou, Alphonse; Wu, Liaowei; Huang, Wei; Gao, Yanhui; Pei, Junrui

doi:10.1007/s12011-021-02617-1

Cited by 4 publications

(4 citation statements)

References 33 publications

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“…Alpha diversity is a measure of the richness and evenness of microbial communities, and prior work has revealed that certain diseases are associated with changes in the α diversity of the microbiome, while others are not [ 35–38 ]. Zhong et al reported the microbial diversity in fecal samples from rat models of dental fluorosis, revealing an initial increase in such diversity (ACE and Chao indices) with rising NaF levels (25 or 50 mg/L for 12 weeks), whereas this diversity fell when higher levels of NaF were provided in the water given to these rats (100 and 150 mg/L groups) [ 21 ]. In the present study, a significant increase in the α diversity in richness of the salivary microbiome was observed in dental fluorosis patients compared with that of control individuals.…”

Section: Discussionmentioning

confidence: 99%

“…For example, the intake of elevated fluoride levels (80 mg/L) can alter both the makeup and diversity of the gut microbiota in common carp, resulting in reduced Actinobacteria abundance with a concomitant increase in Firmicutes and Fusobacterium abundance [ 20 ]. While exposure to a 100 mg/L dose of fluoride does not impact the diversity of the rat fecal microbiome, the richness of these intestinal communities initially rises and then falls as the levels of fluoride in the water rise [ 21 ]. The oral cavity is a highly diverse microbial habitat in humans and home to a relatively stable microbiome that can be impacted by environmental factors, dietary composition, and overall health status [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Saliva microbiome alterations in dental fluorosis population

Liu

Song²,

Zhang

et al. 2023

Journal of Oral Microbiology

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Background We aimed to explore saliva microbiome alterations in dental fluorosis population. Methods The prevalence of dental fluorosis was examined in 957 college students. Dean’s fluorosis index was used to evaluate the dental fluorosis status. Changes in the composition of the salivary microbiome were assessed in a subset of these patients (100 healthy controls, 100 dental fluorosis patients). Results Dental fluorosis affected 47% of the student sample, and incidence was unrelated to gender. Compared with healthy controls, the microbiota of patients with dental fluorosis exhibited increased diversity, with increased abundance of Treponema lecithinolyticum, Vibrio metschnikovii , Cupriavidus pauculus , Pseudomonas , Pseudomonadaceae , Pseudomonadales , and decreased abundance of Streptococcus mutans , Streptococcus sanguinis , Gemella , and Staphylococcales . Function analyses showed increases in arginine biosynthesis in patients affected by dental fluorosis, together with reductions in amino sugar and nucleotide sugar metabolism, fructose and mannose metabolism, and starch and sucrose metabolism. Conclusions These results suggest that there are striking differences in salivary microbiome between healthy controls and dental fluorosis patients. Dental fluorosis may contribute to periodontitis and systemic lung diseases. There is a need for cohort studies to determine whether altering the salivary microbiota in dental fluorosis patients can alter the development of oral or systemic diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Saliva microbiome alterations in dental fluorosis population

Liu

Song²,

Zhang

et al. 2023

Journal of Oral Microbiology

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“…Roseburia can ferment a variety of carbohydrates, increase the content of butyric acid in the intestine, and prevent or treat obesity-related diseases. Studies showed that Turicibacter and Roseburia had a negative association with intestinal dystrophy ( Zhong et al, 2022 ). Combining the research findings, it is concluded that FFCT altered the structure of GM while maintaining enteral nutrition.…”

Section: Discussionmentioning

confidence: 99%

Disordered Gut Microbiota in Colorectal Tumor-Bearing Mice Altered Serum Metabolome Related to Fufangchangtai

Cai

Xiao²,

Lin³

et al. 2022

Front. Pharmacol.

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Purpose: This study aimed to investigate the relationship between gut microbiota (GM) and serum metabolism using antineoplastic Fufangchangtai (FFCT) as the model prescription in the treatment of colorectal cancer (CRC).Methods: Tumor-bearing mice and normal mice were administered different doses of FFCT. The tumor volume of tumor-bearing mice was observed. The levels of CD4+ and CD8+ T cells in the blood, spleen, and tumor of mice were determined using a flow cytometer. The bacterial microbiota in stool samples from mice and the serum metabolomics of FFCT-treated mice and fecal microbiota transplantation mice were detected using 16s RNA sequencing and liquid chromatography–mass spectrometry (LC/MS), respectively.Results: The tumor volume of mice showed no significant decrease after FFCT intervention. The levels of CD4+ and CD8+T lymphocytes showed a significant increase under the intervention of FFCT. GM of colorectal tumor-bearing mice and healthy mice were determined, and the diversity and abundance of Firmicutes, Deferribacteres, Bacteroidetes, and Proteobacteria were significantly different between the two groups. Furthermore, we found that the levels of matrine, isogingerenone B, and armillaripin were significantly decreased in tumor-bearing mice after FFCT intervention, indicating that the tumor-induced dysbiosis of gut bacteria may affect the absorption and metabolism of FFCT. Under the intervention of FFCT, serum metabolism of mice transplanted with feces from CRC patients showed less metabolites related to FFCT than that from healthy people, indicating that GM could be a single factor affecting the metabolism of FFCT. Furthermore, we found that different doses of FFCT-treated mice had higher abundance of Roseburia, Turicibacter, and Flexispira than that in the non-intervention control group. Firmicutes and Bacteroidetes in FFCT-treated groups showed a similar trend compared to the healthy group, indicating that FFCT might correct the intestinal microenvironment by modulating gut microbiota in colorectal tumor-bearing mice.Conclusion: The dysbiosis of GM in tumor-bearing mice reduced the serum metabolites related to FFCT, and FFCT could correct the disordered GM of colorectal tumor-bearing mice to exert efficacy.

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“…Building on preliminary research on the effects of drinking water fluoride on rat gut microbiota [17], this study aims to analyze the dose-response relationship of fluoride exposure in drinking water to the rat gut microbiota. Initially, 16S rRNA amplicon sequencing will be employed to observe changes in the gut microbiota and explore dose-response relationships.…”

Section: Introductionmentioning

confidence: 99%

The Dose–Response Effect of Fluoride Exposure on the Gut Microbiome and Its Functional Pathways in Rats

Mo,

Wang,

Meng

et al. 2023

Metabolites

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Metabolic activities within the gut microbiome are intimately linked to human health and disease, especially within the context of environmental exposure and its potential ramifications. Perturbations within this microbiome, termed “gut microbiome perturbations”, have emerged as plausible intermediaries in the onset or exacerbation of diseases following environmental chemical exposures, with fluoride being a compound of particular concern. Despite the well-documented adverse impacts of excessive fluoride on various human physiological systems—ranging from skeletal to neurological—the nuanced dynamics between fluoride exposure, the gut microbiome, and the resulting dose–response relationship remains a scientific enigma. Leveraging the precision of 16S rRNA high-throughput sequencing, this study meticulously examines the ramifications of diverse fluoride concentrations on the gut microbiome’s composition and functional capabilities within Wistar rats. Our findings indicate a profound shift in the intestinal microbial composition following fluoride exposure, marked by a dose-dependent modulation in the abundance of key genera, including Pelagibacterium, Bilophila, Turicibacter, and Roseburia. Moreover, discernible alterations were observed in critical functional and metabolic pathways of the microbiome, such as D-lyxose ketol-isomerase and DNA polymerase III subunit gamma/tau, underscoring the broad-reaching implications of fluoride exposure. Intriguingly, correlation analyses elucidated strong associations between specific bacterial co-abundance groups (CAGs) and these shifted metabolic pathways. In essence, fluoride exposure not only perturbs the compositional equilibrium of the gut microbiota but also instigates profound shifts in its metabolic landscape. These intricate alterations may provide a mechanistic foundation for understanding fluoride’s potential toxicological effects mediated via gut microbiome modulation.

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Effect of Fluoride in Drinking Water on Fecal Microbial Community in Rats

Cited by 4 publications

References 33 publications

Saliva microbiome alterations in dental fluorosis population

Saliva microbiome alterations in dental fluorosis population

Disordered Gut Microbiota in Colorectal Tumor-Bearing Mice Altered Serum Metabolome Related to Fufangchangtai

The Dose–Response Effect of Fluoride Exposure on the Gut Microbiome and Its Functional Pathways in Rats

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