Effect of Flavonoids on the Differentiation of 3T3-L1 Adipocytes.

Iwashita, Keiko; Yamaki, Kohji; Tsushida, Tojiro

doi:10.3136/fstr.7.154

Cited by 17 publications

(15 citation statements)

References 23 publications

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“…Further studies will be needed to determine the mechanism and the relation of the chemical structure of the fl avonoids and the activity. Interestingly, some fl avonoids such as quercetin, kaempferol, isorhamnetin have also been reported as having antiadipogenesis activity (Iwashita et al, 2001). …”

Section: Discussionmentioning

confidence: 98%

Vitexin, orientin and other flavonoids from Spirodela polyrhiza inhibit adipogenesis in 3T3‐L1 cells

Kim

Lee

Seo

et al. 2010

Phytotherapy Research

104

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To investigate the adipogenesis inhibitory effect on lipid accumulation, 3T3-L1 cells were treated with fractions and isolated flavonoids of Spirodela polyrhiza. An ethanol extract of S. polyrhiza was fractionated into three fractions. The butanol soluble fraction (SPB) exhibited potent antiadipogenesis activity and decreased C/EBPα and PPARγ protein expression level in 3T3-L1 cells without significant cytotoxicity. The flavonoids were isolated from SPB and their chemical structures were identified as chrysoeriol (1), apigenin (2), luteolin (3), vitexin (4), cosmosin (5), orientin (6) and luteolin-7-O-β-d-glucoside (7) by spectroscopic analysis. Studies on the adipogenesis and intracellular triglyceride accumulation inhibitory effect showed that compounds 4 and 6 had the highest activity and decreased C/EBPα and PPARγ protein expression level in 3T3-L1 cells. These results suggest that the flavonoids isolated from SPB, especially compounds 4 and 6, contribute to the inhibitory activity of S. polyrhiza in 3T3-L1 cells.

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Section: Discussionmentioning

confidence: 98%

Vitexin, orientin and other flavonoids from Spirodela polyrhiza inhibit adipogenesis in 3T3‐L1 cells

Kim

Lee

Seo

et al. 2010

Phytotherapy Research

104

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“…The authors suggested the antidifferentiation by quercetin to be the direct inhibition of glyceraldehyde‐3‐phosphate dehydrogenase, the key enzyme in lipid synthesis. Other flavonoids that have been reported to have inhibiting effects were naringenin, hesperetin and phloretin (Iwashita et al . 2001).…”

Section: Resultsmentioning

confidence: 99%

The Effects of Morinda Citrifolia, Momordica Charantia and Centella Asiatica Extracts on Lipoprotein Lipase and 3t3-L1 Preadipocytes

Sahib

Hamid

Kitts

et al. 2010

Journal of Food Biochemistry

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The effect of Morinda citrifolia fruit (MCFE), Momordica charantia (MCE) and Centella asiatica (CAE) extracts on lipoprotein lipase (LPL) activity, proliferation and differentiation of 3T3-L1 preadipocytes was investigated. The inhibitory effect of the extracts on LPL activity was determined in vitro. The extent of cell proliferation was determined utilizing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and the degree of differentiation was measured by Oil Red O staining assay. Extracts were analyzed for total phenolic compounds and flavonoids utilizing Folin-Ciocalteu assay and high-performance liquid chromatography, respectively. Results showed that, at 1 mg/mL, MCFE demonstrated the highest inhibitory effect (21.4 Ϯ 2.3%), compared to the other extracts tested. On the other hand, MCE demonstrated the highest inhibitory effect on both proliferation and differentiation of preadipocytes (LC 50 of 1.6 mg/L). LC 50 refers to the concentration of extracts (mg/mL) to cause a 50% reduction in viable cells. Results also revealed that all extracts consisted of high concentration of phenolic compounds, with catechin and epicatechin predominantly present in all three extracts, that maybe responsible for the activities measured.3 Corresponding

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“…These compounds included the six flavonols, quercetin (5), 25) isorhamnetin (6), 26) rhamnetin (7), 27) quercitrin (8), 25) kaempferol (9), 25) and afzelein (10), 28) the five flavons, chrysoeriol (11), 29) apigenin (12), 30) tricin (13), 31) luteolin (14), 32) and amentoflavone (15), 33) the flavanonol, taxifolin (16), 34) the chalcone, isoliquiritigenin (17), 35) the dithiodicarboxylic acid, 3,3′-dithiodipropanoic acid (18), 36) the indole derivative, indole-3-carboxylic acid (19), 37) the alloxazine derivative, 7,8-dimethylalloxazine (20), 38) the phenylpropanoid, (E)-p-coumaric acid (21), 39) and the mono-terpenoid, (6S)-menthiafolic acid (22). 40) Compounds 6, 7, 11-22 were isolated for the first time from the flower of A. julibrissin.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, natural products also have been reported as a subject for an obesity study in 3T3-L1 cells. Previously, quercetin, 19,20) berberine, 21) ginsenosides, 22) and curcumin, 23) on lipid accumulation in 3T3-L1 adipocytes was investigated. Consequently, crude drugs and natural products with similar properties would help to solve obesity problems worldwide.…”

mentioning

confidence: 99%

Flavonol Acylglycosides from Flower of Albizia julibrissin and Their Inhibitory Effects on Lipid Accumulation in 3T3-L1 Cells

2012

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Obesity is a serious health problem worldwide. We investigated the anti-obesity effect of the flower of Albizia julibrissin DURAZZ. (Leguminosae). A 90% EtOH extract of the flower inhibited adipogenesis in 3T3-L1 preadipocytes, as well as the activity of glycerol-3-phosphate dehydrogenase (GPDH) activity. New flavonol acylglycosides (1-4) and eighteen known compounds (5-22) were isolated by bioassay-directed fractionation. These new glycosides were elucidated to be 3″-(E)-p-coumaroylquercitrin (1), 3″-(E)-feruloylquercitrin (2), 3″-(E)-cinnamoylquercitrin (3), and 2″-(E)-cinnamoylquercitrin (4) on the basis of spectroscopic and chemical analysis. These compounds inhibited adipogenesis in 3T3-L1 preadipocytes. In particular, 2 exhibited potent inhibitory effects on triglyceride accumulation. Furthermore, GPDH activity was inhibited by 2. Additionally, 2 inhibited glucose uptake in 3T3-L1 adipocytes. These results indicate that the 90% EtOH extract and compounds isolated from the flower of A. julibrissin inhibit adipogenesis in 3T3-L1 preadipocytes and may have anti-obesity effect through the inhibition of preadipocyte differentiation.

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Effect of Flavonoids on the Differentiation of 3T3-L1 Adipocytes.

Cited by 17 publications

References 23 publications

Vitexin, orientin and other flavonoids from Spirodela polyrhiza inhibit adipogenesis in 3T3‐L1 cells

Vitexin, orientin and other flavonoids from Spirodela polyrhiza inhibit adipogenesis in 3T3‐L1 cells

The Effects of Morinda Citrifolia, Momordica Charantia and Centella Asiatica Extracts on Lipoprotein Lipase and 3t3-L1 Preadipocytes

Flavonol Acylglycosides from Flower of Albizia julibrissin and Their Inhibitory Effects on Lipid Accumulation in 3T3-L1 Cells

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