Effect of Fibroblast-Seeded Artificial Dermis on Wound Healing

Abstract

In covering wounds, efforts should include use of the safest and least invasive methods with a goal of achieving optimal functional and cosmetic outcome. The recent development of advanced technology in wound healing has triggered the use of cells and/or biological dermis to improve wound healing conditions. The purpose of the study was to evaluate the effects of fibroblast-seeded artificial dermis on wound healing efficacy.Ten nude mice were used in this study. Four full-thickness 6-mm punch wounds were created on the dorsal surface of each mouse (total, 40 wounds). The wounds were randomly assigned to one of the following 4 treatments: topical application of Dulbecco phosphate-buffered saline (control), human fibroblasts (FB), artificial dermis (AD), and human fibroblast-seeded artificial dermis (AD with FB). On the 14th day after treatment, wound healing rate and wound contraction, which are the 2 main factors determining wound healing efficacy, were evaluated using a stereoimage optical topometer system, histomorphological analysis, and immunohistochemistry.The results of the stereoimage optical topometer system demonstrated that the FB group did not have significant influence on wound healing rate and wound contraction. The AD group showed reduced wound contraction, but wound healing was delayed. The AD with FB group showed decreased wound contraction without significantly delayed wound healing. Histomorphological analysis exhibited that more normal skin structure was regenerated in the AD with FB group. Immunohistochemistry demonstrated that the AD group and the AD with FB group produced less α-smooth muscle actin than the control group, but this was not shown in the FB group.Fibroblast-seeded artificial dermis may minimize wound contraction without significantly delaying wound healing in the treatment of skin and soft tissue defects.

Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing

“…This finding is consistent with Jang et al . 41 where the control group, treated with DPBS without cells, exhibited the greatest amount of α-SMA compared to fibroblasts alone, Matriderm (collagen and elastin scaffold), and Matriderm with fibroblasts treated groups. In the granulation tissue during wound healing, fibroblasts are activated to become myofibroblasts which possesses contractile ability.…”

Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement

“…The contracted area of each wound was measured and calculated as previously described 29 , and the rate of wound contraction was calculated as follows:…”

Novel bilayer wound dressing composed of silicone rubber with particular micropores enhanced wound re-epithelialization and contraction

“…Besides, the contracted area was calculated as previously described [18], and the amount of wound contraction was calculated as follows:…”