Effect of fiber alignment in electrospun scaffolds on keratocytes and corneal epithelial cells behavior

Jing, Yan; Qiang, Linhui; Gao, Yan; Cui, Xuejun; Zhou, Hongyan; Zhong, Shuangling; Wang, Qing; Wang, Hongyan

doi:10.1002/jbm.a.33301

Cited by 85 publications

(74 citation statements)

References 25 publications

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“…We have seen distinct morphological differences in cells grown on different architectures, similar to other findings (Yan et al 2012;Mahjour et al 2016;Huang et al 2016). Our random scaffolds show cells in a more rounded morphology, spread between numerous fibres.…”

Section: Discussionsupporting

confidence: 88%

“…These experiments using electrospun scaffolds did not consider the mechanical or physical properties of the scaffolds and the effect this has on the cells. It is well documented that changes is fibre diameter and orientation induce a response of the cells in many tissue types (Yan et al 2012;Balguid et al 2009;Wang et al 2012), but the effect on renal cells has been ignored until now. The diameter of the fibre determines the pore size of the scaffold, and this determines how well cells will penetrate into a scaffold (Balguid et al 2009;Baker et al 2008;Ju et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…This variation in morphology has been shown to affect the way cells behave and integrate with the scaffold, larger fibres allow for greater cell integration, nanofibres represent the natural ECM and aligned fibres have shown to guide neural, vascular and cornea cell growth (Yan et al 2012;Balguid et al 2009;Wang et al 2012). Other methods have been proposed to increase the porosity of scaffolds to allow for greater cell integration; dual-spinning using a water soluble sacrificial material allows for a scaffold with greater porosity (Lowery et al 2010;Phipps et al 2012) and techniques such as cell electrospinning enable cell to be directly integrated within the scaffold (Jayasinghe 2013;Townsend-Nicholson & Jayasinghe 2006).…”

Section: Introductionmentioning

confidence: 99%

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The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream.Focus within kidney tissue engineering has been on 2-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas. The variation in morphology of tissue engineering scaffold has been shown to effect the way cells behave and integrate. In this study we examined the cellular response to scaffold architecture of novel electrospun scaffold for kidney tissue engineering. Fibre diameters of 1.10±0.16 µm and 4.49±0.47 µm were used with 3 distinct scaffold architectures. Traditional random fibres were spun onto a mandrel rotating at 250 rpm, aligned at 1800 rpm with novel cryogenic fibres spun onto a mandrel loaded with dry ice rotating at 250 rpm. Human kidney epithelial cells were grown for 1 and 2 weeks. Fibre morphology had no effect of cell viability in scaffolds with a large fibre diameter but significant differences were seen in smaller fibres. Fibre diameter had a significant effect in aligned and cryogenic scaffold. Imaging detailed the differences in cell attachment due to scaffold differences. These results show that architecture of the scaffold has a profound effect on kidney cells; whether that is effects of fibre diameter on the cell attachment and viability or the effect of fibre arrangement on the distribution of cells and their alignment with fibres. Results demonstrate that PCL scaffolds have the capability to maintain kidney cells life and should be investigated further as a potential scaffold in kidney tissue engineering.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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“…After being completely dried in air, samples were coated with gold, and observed by SEM. For CLSM observations, samples were treated with a special care route which was reported elsewhere [27].…”

Section: In Vitro Investigationmentioning

confidence: 99%

Electrospun Porous PDLLA Fiber Membrane Coated with nHA

Qiang

Zhang²,

Feng³

et al. 2018

Applied Sciences

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Featured Application: The PDLLA/nHA composite fibrous membrane has potential application value as scaffold material in tissue engineering.Abstract: Porous poly-D, L-lactic acid (PDLLA) electrospinning fiber membrane was prepared, and nano-hydroxyapatite (nHA) was adsorbed and wrapped into it during the unique shrinking process of the PDLLA fiber membrane to fabricate the PDLLA/nHA composite membrane scaffold for tissue engineering. Compare with the composite fibers prepared by blend electrospinning, most of nHA particles are observed to distribute on the surface of new type composite fibers, which could significantly improve the water wettability and induce the cellular adherence. FTIR analysis indicated that the PDLLA/nHA composite fibrous membrane was formed by physical adsorption. The combination was probed by scanning electron microscope, thermo-gravimetric, water contact angle and mechanical property analysis. It was proved that the nHA particles' content and distribution, surface wettability, modulus and tensile strength of PDLLA/nHA composite fibrous membrane were influenced by the concentration of nHA dispersion and pores on the PDLLA fiber surface. The 10.6 wt % PDLLA/nHA composite fibrous membrane exhibits a more balanced tensile strength (3.28 MPa) and surface wettability (with a water contact angle of 0 • ) of the composite mats. Scanning electron microscope and confocal laser scanning microscopy images of chondrocyte proliferation further showed that the composite scaffold is non-toxic. The adherence and proliferation of chondrocytes on the 10.6 wt % PDLLA/nHA fibrous membrane was significantly improved, compared with PDLLA mat. The 10.6 wt % PDLLA/nHA composite fibrous membrane has potential application value as scaffold material in tissue engineering.

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“…Alignment of organic micro-or nano-fibers is beneficial for enhanced charge transport [231,232,263,264], production of polarized light emission [265,266], improved absorption and photovoltaic properties [123,267], and enhanced crystal properties [268]. Additional benefits of parallel fiber arrangement include directional cell growth [269] and guided cell differentiation [270] as well as the achievement of high-modulus, highstrength fibers, which can be used for heat-resistant and protective clothing [271,272]. The multitude of applications requiring alignment makes evident the need for straightforward and uninvolved processes for producing aligned fiber structures beyond that of templated strategies.…”

Section: Es Fabrication Of Organic Micro-and Nano-fiber Devicesmentioning

confidence: 99%

Electrospinning for nano- to mesoscale photonic structures

et al. 2016

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Abstract:The fabrication of photonic and electronic structures and devices has directed the manufacturing industry for the last 50 years. Currently, the majority of small-scale photonic devices are created by traditional microfabrication techniques that create features by processes such as lithography and electron or ion beam direct writing. Microfabrication techniques are often expensive and slow. In contrast, the use of electrospinning (ES) in the fabrication of microand nano-scale devices for the manipulation of photons and electrons provides a relatively simple and economic viable alternative. ES involves the delivery of a polymer solution to a capillary held at a high voltage relative to the fiber deposition surface. Electrostatic force developed between the collection plate and the polymer promotes fiber deposition onto the collection plate. Issues with ES fabrication exist primarily due to an instability region that exists between the capillary and collection plate and is characterized by chaotic motion of the depositing polymer fiber. Material limitations to ES also exist; not all polymers of interest are amenable to the ES process due to process dependencies on molecular weight and chain entanglement or incompatibility with other polymers and overall process compatibility. Passive and active electronic and photonic fibers fabricated through the ES have great potential for use in light generation and collection in optical and electronic structures/ devices. ES produces fiber devices that can be combined with inorganic, metallic, biological, or organic materials for novel device design. Synergistic material selection and postprocessing techniques are also utilized for broad-ranging applications of organic nanofibers that span from biological to electronic, photovoltaic, or photonic. As the ability to electrospin optically and/or electronically active materials in a controlled manner continues to improve, the complexity and diversity of devices fabricated from this process can be expected to grow rapidly and provide an alternative to traditional resource-intensive fabrication techniques.

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Effect of fiber alignment in electrospun scaffolds on keratocytes and corneal epithelial cells behavior

Cited by 85 publications

References 25 publications

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

Electrospun Porous PDLLA Fiber Membrane Coated with nHA

Electrospinning for nano- to mesoscale photonic structures

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