Effect of Fertility Blend® Administration on the Oocytes Quality and Embryonic Development using assisted Reproductive Technology in Mice

Abstract

Female Fertility Blend ® (FFB) is one of new nutritional supplement that used to enhance the fertility status in women. This supplement containing vitamins, minerals, enzymes, amino acids, all may improve the oocytes quality and ovarian function; at the same time protect oocytes from free radicals damage. The aim of the study is to examine the in vivo effect of FFB on oocyte quality, and in vitro fertilization rate (IVFR), and embryonic development (ED) at early cleavage stages using the mice as a model for human being. Therefore, two groups of mature female mice were involved (20 mouse each). The treated group is daily orally administrated by 3.4mg/kg /body weight from FFB for 10 days and the other groups (the control) were treated with FFB- free distilled water only for the same period. Oocytes were collected and an epididymal sperms from mature fertilized male mice are obtained and in vitro fertilization (IVF) is done. Following 24 and 48hrs from IVF, the FR and ED rate are recorded. This results showed a significant (P<0.05) differences in fertilization rate and embryonic development when treating the female mice with FFB compared to control group. It is concluded that the FFB treatment has a great improvement in oocytes maturation and in vitro fertilization and embryonic development status.

A comparative study of preimplantation embryos development of young and aged mice treated with L-carnitine

“…The horns of the uterus were dissected under a stereomicroscope (Zeiss, Germany). The "flushing and dissection" technique was used to retrieve embryos from uterine horns or oviducts 18,19 . An inverted microscope (TECHNO, Japan) was used to examine the morphology of all retrieved early-stage (i.e., 2-cell) embryos.…”