Effect of ferrate and monochloramine disinfection on the physiological and transcriptomic response of Escherichia coli at late stationary phase

Daer, Sahar; Goodwill, Joseph E.; Ikuma, Kaoru

doi:10.1016/j.watres.2020.116580

Cited by 19 publications

(13 citation statements)

References 60 publications

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“…[ 15 ]. Destruction of the cell plasma membrane, protein degradation, and DNA damage may be the way for disinfectants to inactivate bacteria [ 16 , 17 ]. Studies on the inactivation of bacteria by chlorine-containing disinfectants have indicated that bacteria might be inactivated by the oxidative damage of cell components [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Screening of Staphylococcus aureus for Disinfection Evaluation and Transcriptome Analysis of High Tolerance to Chlorine-Containing Disinfectants

Song

Huang

et al. 2023

Microorganisms

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The nonstandard use of disinfectants can lead to the disinfectant resistance of bacteria and even increase antibiotic resistance. However, compared with the study of antibiotic resistance, studies of bacterial resistance to disinfectants are relatively few in number. In this study, we explored the standard strain screening procedure for the evaluation of disinfection efficacy. Staphylococcus aureus strains with different sources and substrates were selected from the National Pathogen Resource Center of China and screened the standard strains that could evaluate the long-term bacteriostatic effect of the chlorine-containing disinfectants through the determination of the physical properties, genome-based safety evaluation, and disinfection test evaluation. In this process, one S. aureus strain was more resistant to the long-term bacteriostasis of chlorine-containing disinfectants than the other strains. This strain and the standard strain ATCC 6538 were cultured in the medium containing a low concentration of chlorine-containing disinfectant synchronously. Then, comparative transcriptome analysis was carried out to investigate the potential mechanism of a high tolerance to chlorine-containing disinfectants. The pathway of significant differential expression is related to the oxocarboxylic acid metabolic mechanism, amino acid metabolic mechanism, and pyrimidine mechanism, which may be the molecular mechanism of S. aureus evolution to adapt to chlorine-containing disinfectants. Our study established a technical process for screening and evaluating standard strains for disinfection, which also provided a reference for studying the bacterial evolution mechanism toward chlorine tolerance.

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Section: Introductionmentioning

confidence: 99%

Screening of Staphylococcus aureus for Disinfection Evaluation and Transcriptome Analysis of High Tolerance to Chlorine-Containing Disinfectants

Song

Huang

et al. 2023

Microorganisms

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“…PF, a green oxidant, has been used for drinking water disinfection and for suppressing pathogenic microorganisms in soil and groundwater . PF may cause pathogen inactivation via nonselective protein degradation, DNA damage, and cytoplasmic membrane decomposition . Our previous research demonstrated that the PF treatment samples had thousand-fold lower total coliform and Escherichia coli counts than the UP samples. , PF is more effective than NaClO and kalinite for disinfecting drinking water with respect to fecal bacteria, at a lower dosage and with shorter reaction time .…”

Section: Discussionmentioning

confidence: 94%

Chemical Pretreatments and Anaerobic Digestion Shape the Virome and Functional Microbiome in Fecal Sludge

Cui

Wang

Cai

et al. 2023

Environ. Sci. Technol.

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The decomposition and pathogen inactivation of fecal sludge (FS) are vitally important for safely managing onsite sanitation and protecting public and environmental health. However, the microbiome and virome assemblages in FS after chemical and biological treatments remain unclear. Here, we reported the differences in the solid reduction and microbiomes of FS subjected to potassium ferrate (PF), alkali (ALK), and sodium hypochlorite (NaClO) pretreatments and anaerobic digestion (AD). The PF and NaClO pretreatments enhanced FS hydrolysis and pathogen suppression, respectively; AD suppressed Grampositive bacteria. Most of the viromes were those of bacteriophages, which were also shaped by chemical pretreatments and AD. Metatranscriptome analysis revealed distinct gene expression patterns between the PF-and ALK-pretreated FS and the subsequent AD. Differentially expressed gene profiles indicated that genes related to biological processes, molecular functions, and transcriptional regulators were upregulated in ALK-AD and PF-AD samples. These findings suggested that the effect of different treatment technologies on the viral diversity, pathogen abundance, and metabolic function of the core microbiome extends beyond FS decomposition and that the use of combined processes would provide possible alternatives for FS management in pandemic emergencies.

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“…Although the VBNC state is generally regarded as a survival strategy to against adverse conditions [4], the molecular mechanism is less reported. In previous reports, the transcriptomic approach was used to explore the mechanism of VBNC state in E. coli [18][19][20], Brettanomyces bruxellensis [21], V. alginolyticus [22], V. cholerae [23], Corynebacterium diphtheriae [24], P. syringae pv. syringae [25], Enterococcus faecalis [26], V. parahaemolyticus [27], and C. michiganensis [28].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional profiling of Xanthomonas campestris pv. campestris in viable but nonculturable state

Bai

Xu²,

Wang³

et al. 2023

BMC Genomics

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Background Xanthomonas campestris pv. campestris (Xcc) is an important seed-borne plant pathogenic bacteria that can cause a serious threat to cruciferous crops. Bacteria can enter into the viable but non-culturable (VBNC) state under stress conditions, and cause potential risks to agricultural production because the VBNC bacterial cells will evade culture-based detection. However, little is known about the mechanism of VBNC. Our previous study showed that Xcc could be induced into VBNC state by copper ion (Cu2+). Results Here, RNA-seq was performed to explore the mechanism of VBNC state. The results indicated that expression profiling was changed dramatically in the different VBNC stages (0 d, 1 d, 2 d and 10 d). Moreover, metabolism related pathways were enriched according to COG, GO and KEGG analysis of differentially expressed genes (DEGs). The DEGs associated with cell motility were down-regulated, whereas pathogenicity related genes were up-regulated. This study revealed that the high expression of genes related to stress response could trigger the active cells to VBNC state, while the genes involved in transcription and translation category, as well as transport and metabolism category, were ascribed to maintaining the VBNC state. Conclusion This study summarized not only the related pathways that might trigger and maintain VBNC state, but also the expression profiling of genes in different survival state of bacteria under stress. It provided a new kind of gene expression profile and new ideas for studying VBNC state mechanism in X. campestris pv. campestris.

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Effect of ferrate and monochloramine disinfection on the physiological and transcriptomic response of Escherichia coli at late stationary phase

Cited by 19 publications

References 60 publications

Screening of Staphylococcus aureus for Disinfection Evaluation and Transcriptome Analysis of High Tolerance to Chlorine-Containing Disinfectants

Screening of Staphylococcus aureus for Disinfection Evaluation and Transcriptome Analysis of High Tolerance to Chlorine-Containing Disinfectants

Chemical Pretreatments and Anaerobic Digestion Shape the Virome and Functional Microbiome in Fecal Sludge

Transcriptional profiling of Xanthomonas campestris pv. campestris in viable but nonculturable state

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