Effect of fenitrothion on the physical properties of crustacean lipoproteins

Abstract

FIG. 3.Steady-state fluorescence anisotropy (r s ), phase lifetime (τ P ), modulation lifetime (τ M ), rotational correlation time (τ r ), and limiting anisotropy (r ∞ ) of (A) DPH and (B) DPH-PA in HDL-1 of Macrobrachium borellii, measured in the absence or presence of 1, 10, and 20 ppm FS at 10 and 30°C. Student's t-test was used to compare the significance of the differences with respect to the sample without FS: ***P < 0.001, **P < 0.01, *P < 0.05. FIG. 4.Steady-state fluorescence anisotropy (r s ), phase lifetime (τ P ), modulation lifetime (τ M ), rotational correlation time (τ r ), and limiting anisotropy (r ∞ ) of (A) DPH and (B) DPH-PA in HDL-2 of M. borellii, measured in the absence or presence of 1, 10, and 20 ppm FS at 10, and 30°C. Student's t-test was used to compare the significance of the differences with respect to the sample without FS: ***P < 0.001, **P < 0.01, *P < 0.05.

Fenitrothion‐induced structural and functional perturbations in the yolk lipoproteins of the shrimp Macrobrachium borellii

“…FS at high concentrations decreased the τ of DPH by more than 50% and of DPH-PA by about 30% in both LV and LP. This effect was observed in other lipoprotein and lipid systems (13,14) and was attributed to an increased amount of water penetration in the lipid phase owing to packing defects produced by the insecticide. FS also produced an increase in r ∞ of DPH in LVe and LVl, indicating that this insecticide increased the ordering of the neutral lipid core in both LV.…”

“…We have demonstrated that the organophosphate insecticide fenitrothion (FS; O,O-dimethyl O-4-nitro-m-tolyl phosphorothioate) also incorporates into several circulating invertebrate lipoproteins, altering their lipid dynamics, the penetration of water into the lipid phase, and their ability to exchange FA. Differential alterations in these physical properties were found in shrimp lipoproteins with different lipid and apoprotein compositions (13). These changes were also found in spider lipoproteins, e.g., a different basal lipid order as well as lipid/apoprotein ratio (14).…”

“…The ability of LV to transfer palmitic acid to albumin is increased by FS in a concentration-dependent way. It was shown that FS decreased FA uptake by other lipoproteins (13). These facts suggest that FS decreases the capacity and/or affinity of lipoprotein to bind FA, which in turn might be due to a competition between FS and FA for the same binding site or to the alteration of the lipid phase properties produced by FS.…”

“…The importance of FS-lipoprotein interactions can be considered in two ways: (i) Lipoproteins may carry FS to target organs (13), and (ii) FS may alter the transfer of other lipids by the lipoprotein. The latter mechanism was explored in this work by studying the effect of FS on the palmitic acid transfer capacity of LV by using albumin as the acceptor.…”

Bioquímica y fisiología de lipoproteínas en arácnidos

“…En todos estos casos los lípidos predominantes fueron los PL (Komatsu et al, 1993). En el camarón Macrobrachium borellii se describió una HDL de 1, 13 g/ml de densidad, con un 36% de lípidos siendo los PL los predominantes (Garcia et al, 2002a).…”