Effect of feeding on the pharmacokinetics of vilazodone in dogs

Abstract

Vilazodone (VLZ) is a drug approved for the treatment of major depressive disorder in humans but no data are available for dogs. The present study aimed to evaluate the pharmacokinetics of a single oral 40 mg dose of VLZ in healthy Labrador dogs (n=6) in fasted and fed conditions. Dogs were randomly divided in two (n=3) groups in a cross-over study design (2x2). Group I was administered with VLZ at 40 mg/dog after fasting overnight. Group II was fed prior to and after administration of the same dose. A two-week wash-out period was observed. Plasma samples collected underwent LC-MS/MS analysis. VLZ concentrations were quantified in dogs' plasma in two different windows of time: 30 min to 10 h for the fasted group and 4 h to 35 h for the fed group. The values for t1/2z were statistically different between the groups (fed, 4.6 ± 1.1 h vs fasted, 1.7 ± 0.2 h). Tmax drastically changed between the groups (fed, 10 h vs fasted, 1.5 h), while Cmax did not significantly vary (fed, 39.4 ± 5.6 ng/mL vs fasted, 38.7 ± 4.8 ng/mL). The AUC value was always statistically higher in the fed group. As a result, the average relative oral fasted bioavailability of VLZ was low, 28.8 ± 6.1%. In conclusion, feeding can affect the pharmacokinetics of VLZ in the dog.

Ultrafast Bioanalytical Assay for Vilazodone Quantification in Human Plasma Using Vilazodone D8 Internal Standard by Uplc- Ms/ms

“…Thorough literatures survey was carried out to judiciously assess the qualitative and quantitative analytical methods reported for the determination of vilazodone. There are several analytical methods available to estimate vilazodone in bulk pharmaceuticals and biological matrix including HPLC [8][9][10][11][12][13][14][15][16][17], Spectroscopic [18], HPTLC [19], Spectrophotometric [20,21], Spectro uorometric [22] and LC-MS/MS [23][24][25][26][27][28][29][30][31][32]. However, all reported methods other than LC-MS/MS are associated with several limitations such as sensitivity, higher analysis time and only used for the analytical quality control research purposes.…”

“…However, all reported methods other than LC-MS/MS are associated with several limitations such as sensitivity, higher analysis time and only used for the analytical quality control research purposes. Whereas published LC-MS/MS methods are developed in rat plasma [25,26,28,30], dog plasma [29], human plasma [27,31]. All methods described the inadequate validation range which is most crucial for the reliable estimation of plasma concentration and evaluation of pharmacokinetic parameters, usage of high plasma volume and solvent consumptions is quite more.…”