Effect of Feeding a Postbiotic Derived from Saccharomyces cerevisiae Fermentation as a Preharvest Food Safety Hurdle for Reducing Salmonella Enteritidis in the Ceca of Layer Pullets

Abstract

Salmonella Enteritidis is responsible for a significant proportion of foodborne Salmonellosis in the United States and continues to be attributable to table eggs, despite increased federal oversight in recent years. Technologies, including feed additives, continue to be evaluated for pre-harvest application of their potential food safety benefits. Here, Diamond V Original XPC™, a Saccharomyces cerevisiae fermentation based postbiotic (SCFP), was evaluated for its effectiveness in the reduction of Salmonella Enteritidis (SE) colonization in young layer pullets. A total of forty, day-old Hy-Line W-36 layer pullets were equally divided and randomly assigned to one of two dietary treatments with SCFP, or without (PCON) and orally gavaged on Day 28 with 10 6 CFU/mL of SE. Another twenty, day-old layer pullets were fed the same control feed without SCFP and blank inoculated on Day 28 with 1 ml of sterile PBS to serve as a negative control (NCON). Qualitative and quantitative analyses of cecal Salmonella contents were performed for all birds on Day 32. The prevalence of SE in the ceca of all directly challenged birds was 100%, however, the SE concentration in birds fed SCFP diet (3.35 Log 10 CFU/g) was significantly lower ( P <0.0001) than that of the PCON birds not fed SCFP (4.49 Log 10 CFU/g). Moreover, the proportion of SE colonized individuals with enumerable SE concentrations was lower in SCFP fed birds (57.9%) when compared to the PCON (95.0%). These data suggest that inclusion of SCFP in the diet may aid in the reduction of SE within the ceca of commercial laying hens and could serve as an additional, pre-harvest food safety hurdle.

Saccharomyces cerevisiae fermentation product improves robustness of equine gut microbiome upon stress

“…Our results are also in agreement with other studies that demonstrated that postbiotic supplementation is associated with improved microbiome balance which has been translated into host health in many species (23,60,62,63). In horses, postbiotic supplementation resulted in increased relative abundances of fibrolytic bacteria (64) and attenuated exercise-induced stress markers (28).…”

Dietary Inclusion of a Saccharomyces cerevisiae-Derived Postbiotic Is Associated with Lower Salmonella enterica Burden in Broiler Chickens on a Commercial Farm in Honduras

“…Controlled research evaluating the effects of SCFP to reduce Salmonella Enteritidis challenges has demonstrated quantitative reductions in cecal contents [ 62 , 63 ]; however, reductions in prevalence (% positive) for direct challenge studies are commonly not observed due to extremely high challenge doses administered via oral gavage. Real-world industry conditions have exposure routes and doses which vary greatly [ 59 ].…”

Non-Antibiotics Strategies to Control Salmonella Infection in Poultry

“…In contrast to probiotics, postbiotics involve the use of non-viable bacteria or bacterial metabolic products such as inactivated cells, enzymes, exopolysaccharides, plasmalogens, organic acids, short-chain fatty acids, and peptides, mainly (but not exclusively) produced from lactic acid bacteria due to their multiple metabolic abilities [ 59 ]. Some of the benefits offered by postbiotics, when used in poultry, include the direct or indirect control of pathogens such as Salmonella and their negative effects ( Table 4 ).…”