Effect of Extraction Solvent on the Phytoconstituents of &lt;i&gt;Aegle marmelos&lt;/i&gt; (L.) Correa

Sulaiman, C T; Shahida, V.; Balachandran, Indira

doi:10.18311/jnr/2015/498

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…The results in this study show that, the extraction yield increases with increasing polarity of the solvent used, this may be the reason why yields of ethanol, and acetone extracts are higher than yields of water. These results are in agreement with previous studies in which, extraction yields of rice bran and some medicinal plants decreased in the following order: ethanol > acetone > distilled water [22].…”

Section: Discussionsupporting

confidence: 93%

Biological properties and identification of some active ingredients in Anastatica hierochuntica and Lepidium sativum, grown in Egypt

Al-Shimaa Saber Abd-elmegeed,

Heba saad Abd-alrahman,

Asmaa Ahmed Mohamed

et al. 2023

Int. J. Sci. Res. Arch.

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Medicinal plants have been used as a source of therapies since ancient times in Egypt. The present study was designed to investigate and compare between the anti-oxidant, anti-bacterial, and anti-tumor activity of different extracts from Anastatica hierochuntica and Lepidium sativum belong to Brassicaceae, cultivated in Egypt. The qualitative phytochemical screening followed by DPPH (1, 1-diphenyl-2-picrylhydrazyl) assay were used to assess the anti-oxidant of the extracts, while the disk diffusion method followed by micro-broth dilution were used to determine minimum inhibitory concentration of the plant extracts against 6 bacterial strains belonging to 3 species, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, The all results show that the studied plants have various bioactive phytochemicals. Both plants were observed to be moderate antioxidant agents but L. sativum was better than A. hierochuntica. Moreover, all studied crude extracts were able to inhibit the growth of reference and clinical isolated bacteria while L. sativum was more effective. A human ovarian adenocarcinoma OV17R, cell line was used to evaluate antitumor of these extracts by Sulfo-Rhodamine B colorimetric (SRB) assay and IC50 values below 20 μg/mL were recorded for the crude extract of both tested plants. Benzoic acid, cinnamic acid, rutin and vanillin were the most abundant phenolic compounds in extracts. Rutin and benzoic acid showed the best anti-bacterial activity against all tested bacteria, also expressed moderate cytotoxic activity with IC50 28.8 and 37.2 μg/mL, respectively. The present investigation provided supportive data for the possible use of the plant extracts investigated here in treatment of various diseases.

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Section: Discussionsupporting

confidence: 93%

Biological properties and identification of some active ingredients in Anastatica hierochuntica and Lepidium sativum, grown in Egypt

Al-Shimaa Saber Abd-elmegeed,

Heba saad Abd-alrahman,

Asmaa Ahmed Mohamed

et al. 2023

Int. J. Sci. Res. Arch.

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“…The extractive values of all the fractions of the rhizome did not affect the antibacterial potential of the extracts. The results of the yield of C. longa are similar to that of Sulaiman et al [36] where the effect of extraction solvent on the Phytoconstituents of Aegle marmalos showed maximum yield for hydroalcohol extract compared to other extracts. The authors also reported that yield of extract varies with extraction solvent and the part of the plant used.…”

Section: Discussionsupporting

confidence: 86%

The Antimicrobial, Anti Inflammatory and Analgesic Activities of the Rhizome Extract of Curcuma longa L. (Turmeric)

Ify

Raphael

Tochukwu

et al. 2021

JABB

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Aim: This study aimed at investigating the anti-microbial, anti-inflammatory, and analgesic properties of rhizome extracts of Curcuma longa (Turmeric). Methodology: Extraction of biochemical constituents of the 150 g of the turmeric rhizomes powder was carried out successively using hexane, ethyl acetate, methanol, and water as solvents. The antimicrobial potentials of the different fractions of the extract were tested on clinical bacterial including Staphylococcus aureus, Streptococcus pneumonia, Klebsiella pneumonia and Pseudomonas aeruginosa using agar well diffusion method at concentrations of 400 mg/ml, 200 mg/ml, 100 mg/ml, 50 mg/ml, and 25 mg/ml. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using agar dilution technique. For the anti-inflammatory effects of the extract of C. longa, albino rats were treated with methanolic extract (250, 500, and 1000 mg/kg body weight) and diclofenac as control, after which they were injected with egg albumin on the right hind paw to induce inflammation. The swelling degree of the injected paw was measured. The analgesic effect of C. longa methanolic extract on hot plate-induced pain was also investigated in albino rats, and the Index of response latency was recorded. Results: The biochemical assay showed that C. longa extract contained Alkaloids, Saponins, Tannins, Flavonoids, Carbohydrate, Steroids, Terpenes, Anthraquinone, and Cardiac Glycosides, with Methanol and aqueous extracts having the most biochemical constituents. The antibacterial susceptibility screening showed that K.pneumonia was the most susceptible organism, having the highest zone of inhibition of 18 mm for ethyl acetate extract at the concentration of 400 mg/ml. This was followed by S. pneumonia, S.aureus and P. aeruginosa with inhibition zone of 13, 12 and 10 mm respectively. The MIC for K.pneumonia and S.aureus were both 200 mg/ml, while that of P. aeruginosa and S. pneumonia were both 400 mg/ml. The paw size of rats treated with increasing doses of the extract and diclofenac significantly decreased with time. The concentration of 250 mg/kg had the lowest paw volume of 4.39±0.36mm while 1000 mg/kg recorded 5.18±0.28 as the highest, at 180 minute. Conclusion: Based on the result obtained, it can be concluded that Curcuma longa Rhizome extract possess anti-microbial, anti-inflammatory, and analgesic properties.

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“…It proves that ethyl acetate fraction can inhibit the performance of the pancreatic lipase enzyme as an enzyme that plays an important role in fat metabolism and fat absorption. Moreover, ethyl acetate fraction also plays a role in inhibiting α-glucosidase, thus reducing the absorption of carbohydrate [17], [18], [19].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Ethanol and Ethyl Acetate Fraction of Chayote fruit (Sechium edule Jacq. Swartz) on the Oxidative Stress and Insulin Resistance of Male White Rat Model Type 2 Diabetes Mellitus

Siahaan

Illyas

Lindarto

et al. 2020

Open Access Maced J Med Sci

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BACKGROUND: Oxidative stress in type 2 diabetes mellitus (T2D) causes insulin resistance and disordered insulin secretion. Pathomechanisms of T2D consist of dysfunctional pancreatic β-cell and insulin resistance caused by free radical (reactive oxygen species and reactive nitrogen species) that produced from the glucose metabolism pathway. Insulin resistance can be measured using the homeostatic model assessment of insulin resistance (HOMA-IR). Oxidative stress can measure through the activities of malondialdehyde (MDA) and superoxide dismutase (SOD). AIM: This research aims to study the potential of chayote (Sechium edule Jacq. Swartz) to be used as antihyperglycemic in T2D. MATERIALS AND METHODS: This research was conducted with a post-test randomized controlled group design. Eleven groups with four male rats each were used. Normal untreated rats were treated under ad libitum feeding and drinking condition. Meanwhile, the rat models were induced with the combination of 45 mg/kg b.w. streptozotocin, 110 mg/kg b.w. nicotinamide, 40.5 mg/kg b.w. metformin, high-fat diet, and/or chayote extract. The chayote extract was orally administered to the rat in the form of ethanol extract and/or ethyl acetate fraction, with three dosages of 45 mg/kg b.w., 100 mg/kg b.w., and 150 mg/kg b.w. for each extract type. The body weight, glucose level, insulin level, MDA, and SOD activities were measured. The HOMA-IR was used. RESULTS: The lowest body weight of the rat model in week 0 was 145 ± 25.31, founded in Group H that was treated with ethyl acetate fraction of chayote extract (45 mg/kg b.w.). The lowest blood sugar level in the group with 2 h glucose load was 112.5 ± 27.00 on average, found in Group G that was treated with chayote ethanolic extract (150 mg/kg b.w.). The highest SOD in the group treated with chayote extract was 1.27 ± 0.20, founded in Group H treated with ethyl acetate 45 mg/kg b.w. The lowest level of MDA was 0.86 ± 0.70 in Group H treated with ethyl acetate 45 mg/kg b.w. The lowest fasting blood sugar spectrophotometer level was 150.54 ± 17.24 mg/dl in Group K with metformin treatment, followed by 155.16 ± 31.92 mg/dl in Group K treated 45 mg/kg b.w. ethanol treatment. The highest insulin level was 6.14 ± 0.71, founded in Group F that was treated with chayote ethanolic extract 100 mg/kg b.w. The lowest measurement of HOMA-IR was 0.16 ± 0.80 in Group E treated with ethanol extract of chayote 45 mg/kg b.w. CONCLUSION: Ethanol extract and fractionation of chayote work as an antioxidant and anti-insulin resistance.

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Effect of Extraction Solvent on the Phytoconstituents of <i>Aegle marmelos</i> (L.) Correa

Cited by 5 publications

References 12 publications

Biological properties and identification of some active ingredients in Anastatica hierochuntica and Lepidium sativum, grown in Egypt

Biological properties and identification of some active ingredients in Anastatica hierochuntica and Lepidium sativum, grown in Egypt

The Antimicrobial, Anti Inflammatory and Analgesic Activities of the Rhizome Extract of Curcuma longa L. (Turmeric)

The Effect of Ethanol and Ethyl Acetate Fraction of Chayote fruit (Sechium edule Jacq. Swartz) on the Oxidative Stress and Insulin Resistance of Male White Rat Model Type 2 Diabetes Mellitus

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