Effect of Extraction Methods on the Types and Levels of Free Amino Acid of Beef Longissimus Muscle

Dashdorj, Dashmaa; Hwang, Inho

doi:10.5851/kosfa.2012.32.3.369

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…Depending on the extraction method, the constituents of amino acids from different organisms vary [ 33 , 34 , 35 ]. We used three extraction methods in our current study, and although only free amino acids were analyzed, the extracts’ contents were significantly different depending on the extraction method.…”

Section: Discussionmentioning

confidence: 99%

Anti-Stress and Anti-Depressive Effects of Spinach Extracts on a Chronic Stress-Induced Depression Mouse Model through Lowering Blood Corticosterone and Increasing Brain Glutamate and Glutamine Levels

Son

Jung

Shin

et al. 2018

JCM

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Spinach is one of the most widely consumed vegetables, and is known as for both physical and mental health maintenance. However, there is little information about how spinach protects one from stress. In the present study, we created three extracts from Spinach oleracea L., (frozen powder (FP), water extract (WE), and ethanol extract (EE)), and examined their anti-stress and anti-depressive effects on mouse using a chronic immobilization stress (CIS) regimen. FP, WE, and EE showed different free amino acid constituents. Calorie-balanced diets derived from each extract were tested for their ability to reduce blood corticosterone (CORT) levels in naïve mice. Diets supplemented with FP or EE induced lower blood CORT levels than a normal diet, but the WE diet did not. Mobility duration and sucrose preference were increased by FP and EE supplementation in the CIS-induced depression animal models. Moreover, FP and EE increased glutamate and glutamine levels in the medial prefrontal cortex (mPFC) compared with CIS-induced depressed group. These results suggest that spinach has anti-stress and anti-depressive properties by lowering CORT and increasing glutamate and glutamine levels in the mPFC.

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Section: Discussionmentioning

confidence: 99%

Anti-Stress and Anti-Depressive Effects of Spinach Extracts on a Chronic Stress-Induced Depression Mouse Model through Lowering Blood Corticosterone and Increasing Brain Glutamate and Glutamine Levels

Son

Jung

Shin

et al. 2018

JCM

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“…During the separation process, a flow rate of 1.5 mL/min was maintained. The primary amino acids in the samples were quantified by using alpha-aminobutyric acid as an internal standard 62 , 63 .…”

Section: Methodsmentioning

confidence: 99%

“…Composition and amino acid content. Briefly, the estimation of amino acid (TAA and FAA) content in FFS and LFS involved two stages, the sample preparation followed by the analysis of chromatographic data 62 . The content of TAAs and FAAs in two types of ensilages (FFS and LFS) were carried out following the method described by Henderson & Brooks (2010) 63 using Agilent 1260 Infinity System (Agilent Technologies Inc., USA).…”

Section: Estimation Of Principal Biochemical Constituentsmentioning

confidence: 99%

Comparative assessment of amino acids composition in two types of marine fish silage

Gauthankar

Khandeparker

Shivaramu

et al. 2021

Sci Rep

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Fish silage is a brown liquefied product achieved by the action of enzymes when finely grounded whole/parts of either single or mixed fish types are subjected to acidification. This study made a comparative assessment of biochemical and nutritive properties, especially the amino acid composition in supernatant phase of formic acid silages prepared from two fish types, Indian mackerel (Rastrelliger kanagurta) and false travely (Lactarius lactarius) representing fat fish (FF, fat content > 5%) and lean fish (LF, fat content < 5%), respectively during 35 days of fermentation (DoF). Significantly higher content of total amino acid (TAA) and free amino acids (FAA) were recorded in FFS (TAA, 41.2 ± 0.03 mg/g; FAA, 31.3 ± 0.003 mg/g) compared to LFS (TAA, 35.8 ± 0.07 mg/g; FAA, 18.26 ± 0.003 mg/g; FAA, 31.3 ± 0.003 mg/g) (p < 0.05). At the end of 35 DoF, the concentrations of amino acids such as asparagine, histidine, isoleucine, valine, cysteine, serine, lysine and arginine were significantly higher in FFS as compared to LFS. The relative amino acid composition of FFS and LFS varied in accordance with DoF and the relationship was found to be highly significant (ANOVA, p < 0.00001). High concentrations of l-amino acids such as leucine, glutamic acid and arginine were recorded in both FFS and LFS. In conclusion, the analysis suggested that a fermentation period of 25–30 days showed a significant effect on the composition of amino acids in both types of ensilage compared to other fermentation periods (p < 0.05). Considering the role of amino acids in enhancing the plant growth and proliferation, the findings of the present study are quite useful.

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Assessing the Changes in Protein Content During the Ensilage of the Indian Mackerel (Rastrelliger kanagurta) by Biological Method

Mahale

Khandeparker

Gauthankar

et al. 2023

Waste Biomass Valor

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Fish silage is a lique ed product resulting from tissue hydrolysis and the richness of the silage is characterized by its nutritional pro le comprising of proteins, amino acids, and fats. For the production of sh silage of high nutritional value, a precise assessment of the degree of hydrolysis, particularly proteolysis becomes a paramount importance. The goal of this study is to assess the changes in protein content in sh silage prepared from the Indian mackerel, (Rastrelliger kanagutra) waste using 10% (v/w) sugarcane molasses, during 8 days of fermentation (DoF). The resultant ensiled product was evaluated for changes in titratable acidity, nutrients, total protein, total carbohydrate, total lipids content, and amino acid composition. Altogether, 17 types of amino acids were identi ed. Concentrations of total amino acids (15.627 ± 0.03 mg/g) and free amino acids (5.556 ± 0.08 mg/g) peaked at the end of 6 DoF with the predominance of histidine, arginine, alanine, asparagine, aspartic acid, and glutamic acid. Results of the non-metric multidimensional scaling (nMDS) plots and one-way analysis of similarities (ANOSIM) test indicated signi cant differences in the composition of total amino acids at different DoF were discernible (nMDS, stress 0.03162, one-way ANOSIM R = 1; p < 0.0001). Similarity percentage (SIMPER) analysis indicated that maximum content of amino acids (TAA and FAA) were released in silage during 4-6 DoF. A signi cant difference was observed in concentrations of most amino acids except cysteine, serine, and tryptophan (ANOVA, p < 0.05) at 2,4,6 and 8 DoF. Considering the vital role played by amino acids in providing resistance to plant pathogens as well as contributing to animal nutritional supplements, the results of the present study are highly noteworthy. Statement Of NoveltyThe current study was performed to explore the approach for the preparation of biological sh silage for different DoF. Liqui ed sh silage by the biological method was rapid with a shorter incubation period (4-6 DoF) as compared to others documented in the literature. Biological sh silage preparation has explicit bene ts in terms of ease of preparation and environment-friendly approach. Amino acids such as asparagine and glutamine possessing plant growth promotion property and role in animal nutrition hitherto unreported in formic-acid sh silage were recorded in the sh silage prepared by the biological method. Therefore method used in the current study, employing 10 % molasses for the preparation of biological sh silage can be used as an important alternative to the safe and environmentally friendly disposition of waste material generated from the sh industry.

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Effect of Extraction Methods on the Types and Levels of Free Amino Acid of Beef Longissimus Muscle

Cited by 5 publications

References 10 publications

Anti-Stress and Anti-Depressive Effects of Spinach Extracts on a Chronic Stress-Induced Depression Mouse Model through Lowering Blood Corticosterone and Increasing Brain Glutamate and Glutamine Levels

Anti-Stress and Anti-Depressive Effects of Spinach Extracts on a Chronic Stress-Induced Depression Mouse Model through Lowering Blood Corticosterone and Increasing Brain Glutamate and Glutamine Levels

Comparative assessment of amino acids composition in two types of marine fish silage

Assessing the Changes in Protein Content During the Ensilage of the Indian Mackerel (Rastrelliger kanagurta) by Biological Method

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