Effect of extraction conditions on the antioxidant and antiglycation capacity of carbohydrates from Opuntia robusta cladodes

Abstract

The extraction and characterisation of six crude polysaccharides from Opuntia robusta cladodes, as well as the assessment of their antioxidant and antiglycation activities in vitro, were examined. In this work, carbohydrates were extracted from defatted cladode's peels and pulps through a sequential extraction method in different aqueous medium (neutral, basic and chelating). The obtained polysaccharides were compared in terms of yield and physicochemical properties. Water-soluble extracts showed the highest yield, while peel's chelating-soluble extract gave high galacturonic acid content. Preliminary antioxidant in vitro tests suggested that peel's chelating-soluble extract presented the highest scavenging activity towards DPPH free radicals and inhibition of linoleic acid peroxidation. Furthermore, all extracted polysaccharides showed good potential in inhibiting the nonenzymatic glycation reaction, particularly on the first 3 days of incubation. The obtained results suggested that Opuntia robusta extracts could be explored as natural antioxidant, especially those extracted with chelating agent.

Purification and identification of rambutan (Nephelium lappaceum) peel phenolics with evaluation of antioxidant and antiglycation activities in vitro

“…Inhibition on the formation of Amadori products Amadori products were produced during the initial stage of protein nonenzymic glycation progress (Chaouch et al, 2016). Amadori products could reduce NBT to coloured compounds, which showed maximum absorbance at 530 nm.…”

Structural characterization and hypolipidemic activities of purified stigma maydis polysaccharides

“…Recently, natural polysaccharides have attracted much attention in the field of biochemistry and pharmacology because of their health benefits such as immune regulation (Li et al, ; Surin, Surayot, Seesuriyachan, You, & Phimolsiripol, ), antitumor (Meng, Liang, & Luo, ; Zhao et al, ), antifatigue (Jia et al, ), antioxidation (Chaouch, Hafsa, Rihouey, Cerf, & Majdoub, ; Mao et al, ), antidiabetes (Liu, Sun, Rao, Su, Li, et al, ), antihyperglycemia, and antihyperlipidemia (Liu, Sun, Rao, Su, & Yang, ). Stigma maydis (corn silk), a by‐product from corn cultivation and processing (Žilić, Janković, Basić, Vančetović, & Maksimović, ), has been used worldwide as a traditional herbal medicine for the treatment of chronic diseases, such as diabetes (Pan et al, ), obesity (Lee et al, ), hypertension (George & Idu, ), and cystitis (Peng, Zhang, & Zhou, ), due to its multiple bioactive components including polysaccharides, flavonoids, steroids, alkaloids, saponins, proteins, volatile oils, tannins, and vitamins (Zhao et al, ).…”

Preparation, characterisation and antioxidant activities of litchi (Litchi chinensis Sonn.) polysaccharides extracted by ultra‐high pressure

“…The FT‐IR analysis was carried out using the potassium bromide (KBr)‐disc method as described by Chaouch et al . () and Jiménez‐Escrig et al . () with a slight modification.…”

“…FT-IR is often used to determine functional groups, chemical structure, monosaccharides composition and structure of hemiacetal hydroxyl in polysaccharides. The FT-IR analysis was carried out using the potassium bromide (KBr)-disc method as described by Chaouch et al (2016) and Jim enez-Escrig et al (2015) with a slight modification. LCP (3 mg) was mixed with dried potassium bromide powders and ground for 5 min in agate mortar.…”