Effect of extracellular matrix on adhesion, viability, actin cytoskeleton and K+ currents of cells expressing human ether à go-go channels

Toral, Claudia; Mendoza-Garrido, M. Eugenia; Azorin, E; Hernández-Gallegos, Elizabeth; Gómora, Juan Carlos; Delgadillo, Dulce María; Solano-Agama, Carmen; Camacho, Javier

doi:10.1016/j.lfs.2007.05.014

Cited by 34 publications

(27 citation statements)

References 29 publications

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“…Influence of potassium on cancer survival is poorly investigated. There are few reports suggesting extracellular matrix ether à go-go (EAG) potassium channels to provide oncogenic properties [27]. In vitro studies revealed increased tumour cell proliferation by activation of EAG channels.…”

Section: Discussionmentioning

confidence: 99%

Renal cell carcinoma and tumour thrombus in the inferior vena cava: clinical outcome of 98 consecutive patients and the prognostic value of preoperative parameters

Niedworok

Dörrenhaus

Dorp³

et al. 2014

World J Urol

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Section: Discussionmentioning

confidence: 99%

Renal cell carcinoma and tumour thrombus in the inferior vena cava: clinical outcome of 98 consecutive patients and the prognostic value of preoperative parameters

Niedworok

Dörrenhaus

Dorp³

et al. 2014

World J Urol

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“…The relationship of intracellular water and the cytoskeleton, however, has been reviewed [344]. The amount of "free" versus "bound" water [345], K + ions and the cytoskeleton are all involved in the differences between normal and cancer cells [346]. It can be inferred therefore that the critical distinction between normal and cancer cells lies in a subtle imbalance in intracellular and extracellular interfacial water tension.…”

Section: Eiws and Diseasementioning

confidence: 99%

Biological Water Dynamics and Entropy: A Biophysical Origin of Cancer and Other Diseases

Davidson¹,

Lauritzen

Seneff

2013

Entropy

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This paper postulates that water structure is altered by biomolecules as well as by disease-enabling entities such as certain solvated ions, and in turn water dynamics and structure affect the function of biomolecular interactions. Although the structural and dynamical alterations are subtle, they perturb a well-balanced system sufficiently to facilitate disease. We propose that the disruption of water dynamics between and within cells underlies many disease conditions. We survey recent advances in magnetobiology, nanobiology, and colloid and interface science that point compellingly to the crucial role played by the unique physical properties of quantum coherent nanomolecular clusters of magnetized water in enabling life at the cellular level by solving the "problems" of thermal diffusion, intracellular crowding, and molecular self-assembly. Interphase water and cellular surface tension, normally maintained by biological sulfates at membrane surfaces, are compromised by exogenous interfacial water stressors such as cationic aluminum, with consequences that include greater local water hydrophobicity, increased water tension, and interphase stretching. The ultimate result is greater "stiffness" in the extracellular matrix and either the "soft" cancerous state or the "soft" neurodegenerative state within cells. Our hypothesis provides a basis for understanding why so many idiopathic diseases of today are highly stereotyped and pluricausal. OPEN ACCESSEntropy 2013, 15 3823

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“…In the third trimester, up to 90% of cervical collagen is soluble; thus, its concentration decreases w50% compared with non-pregnant women , Myers et al 2009). Human KCNH1-mediated potassium currents are decreased in cells grown on collagen (Toral et al 2007). Thus, a plausible scenario is that the up-regulating effects of P 4 on KCNH1 mRNA and protein expression are related to the inhibitory effect of P 4 on collagen organisation/ concentration.…”

Section: Discussionmentioning

confidence: 99%

KCNH1 potassium channels are expressed in cervical cytologies from pregnant patients and are regulated by progesterone

et al. 2013

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Potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1) potassium channels are potential tumour markers and cancer therapeutic targets and are up-regulated by oestrogens and human papilloma virus (HPV) oncogenes. However, the role of KCNH1 in normal tissues is poorly understood, and its expression in pregnancy is unknown. We wondered whether KCNH1 channels are expressed in cervical cells from pregnant patients and whether progesterone (P 4 ) regulates KCNH1. The association with HPV was also investigated. KCNH1 protein expression was studied by immunocytochemistry in liquid-based cervical cytologies; 93 samples were obtained from pregnant patients at different trimesters, and 15 samples were obtained from non-pregnant women (controls). The presence of HPV was studied by PCR with direct sequencing and nested multiplex PCR. HeLa cervical cancer cells were transfected with human progesterone receptor-B (PR-B) and treated with P 4 . KCNH1 mRNA expression in these cultures was studied by real-time PCR. KCNH1 protein was detected in 100% of the pregnancy samples and in 26% of the controls. We found 18 pregnant patients infected with HPV and detected 14 types of HPV. There was no association between the percentage of cells expressing KCNH1 and either the presence or type of HPV. P 4 induced KCNH1 mRNA and protein expression in cells transfected with human PR-B. No regulation of KCNH1 by P 4 was observed in non-transfected cells. We show for the first time the expression of an ion channel during human pregnancy at different trimesters and KCNH1 regulation by P 4 in human cells. These data raise a new research field for KCNH1 channels in human tissues.

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Effect of extracellular matrix on adhesion, viability, actin cytoskeleton and K+ currents of cells expressing human ether à go-go channels

Cited by 34 publications

References 29 publications

Renal cell carcinoma and tumour thrombus in the inferior vena cava: clinical outcome of 98 consecutive patients and the prognostic value of preoperative parameters

Renal cell carcinoma and tumour thrombus in the inferior vena cava: clinical outcome of 98 consecutive patients and the prognostic value of preoperative parameters

Biological Water Dynamics and Entropy: A Biophysical Origin of Cancer and Other Diseases

KCNH1 potassium channels are expressed in cervical cytologies from pregnant patients and are regulated by progesterone

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