Effect of Experimentally Induced Liver Cirrhosis on Wound Healing of the Post-Extraction Tooth Socket in Rats

Abstract

Background: Wound healing in liver cirrhosis is known to be impaired possibly due to liver insufficiency and subsequent malnutrition status; however, there is no study to examine healing effectiveness of the tooth socket following an extraction in such patients. Materials and Methods: Irreversible cirrhosiswas induced in 30 Wistar rats by repetitive weekly doses of CCl₄ and continuous administration of phenobarbital in a 12-week course was monitored by body weight measurement and ascites development, and was proved histologically. One week later, cirrhotic and control rats were subjected to extractions of two maxillary grinders on each side, one side by simple method, the other by surgical method. Half of the animals of each subgroup were sacrificed on the 10th post-extraction day, whereas the other half on the 30th post-extraction day, and histological sections were examined from all tooth sockets for wound-healing activity. Results: A malnutrition status was detected in cirrhotic rats with significant difference in their body weight. Several histological parameters of socket healing were not statistically different between cirrhotic and control animals. However, a significant delay on epithelialization and cancellous bone formation was detected on the 10th post-extraction day for either simple or surgical extractions in cirrhotic animals. Conclusions: Liver cirrhosis in rats provokes a significant delay on epithelialization and mature cancellous bone formation and consecutively on early socket wound healing after a tooth extraction.

Pre-clinical Models for Oral and Periodontal Reconstructive Therapies

“…The small size of the extraction socket and the strong innate healing capacity, however, make this model less suitable for the screening of NMF. Thus, most studies conducted with this model focus on the impact of systemic variables on the repair of extraction sites (Pereira et al, 2007;Karalis et al, 2008;Wu et al, 2008). Potential endpoints include the rate and amount of new bone formation, resorption of the alveolar ridge, and consolidation of implanted biomaterials (Hahn et al, 1988;Hile et al, 2005;Balabanian et al, 2006).…”

Cigarette smoke inhalation modulates gene expression in sites of bone healing: a study in rats

“…The healing events in tooth extraction sockets culminate in the formation of woven bone that remodels and results in restoration of the defect. 1 Smoking has been reported to negatively affect bone healing after tooth extraction, leading to a higher incidence of pain, 2 reduced socket blood fill, decreased bony height, 3,4 and low bone mineral density. 3 Such clinical findings have also been histologically documented in rats.…”

Early Propranolol Administration Does Not Prevent Development of Esophageal Varices in Cirrhotic Rats

“…Cirrhosis was induced in rats that had previously undergone ligation of the left adrenal vein and complete devascularization of the left renal vein. The effectiveness of this model has already been demonstrated in previous studies [12,13] .…”

“…Liver cirrhosis, portal hypertension and esophageal varices were induced using a model, originally developed in our department, which has been proved to be very effective for the induction of cirrhotic portal hypertension and esophageal varices [12,13] .…”

“…Two weeks later, induction of liver cirrhosis started according to the model of weekly intragastric administration of CCl 4 in the phenobarbitone-treated rat [14,15] . CCl 4 was administered weekly until stable ascites developed (8-10 weeks) as previously described [13] . Ascites development was easily recognized by an abrupt daily increase in body weight and was confirmed by the abdominal distention observed in the anesthetized rat in the prone position.…”