Effect of experimental conditions on the binding abilities of ciprofloxacin-imprinted nanoparticles prepared by solid-phase synthesis

Effect of Polymerization Time on the Binding Properties of Ciprofloxacin-Imprinted nanoMIPs Prepared by Solid-Phase Synthesis

“…Glass beads (Spheriglass-2429, 70–100 μm average particle size, Potters, UK) were aminated as previously reported [ 22 ]. Acrylic acid (AA), 3-(aminopropyl) trimethoxysilane (APTMS), ciprofloxacin (CIP), N , N -dimethylaminopyridine (DMAP), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), hexamethyldisilazane (HMDS), N -hydroxysuccinimide (NHS), N -isopropylacrylamide (NIPAm), levofloxacin (LEV), N , N ′-methylene-bis-acrylamide (BIS), morpholinethanesulfonic acid (sodium salt, MES), succinic anhydride, N -tertbutylacrylamide (TBAm), ammonium persulfate (APS), N , N , N ′, N ′-tetramethylethylenediamine (TEMED) were obtained from Sigma-Merck (Milan, Italy).…”

“…The template CIP-HS was grafted onto the aminated glass beads in accordance with the protocol previously reported [ 22 ]. The polymerization mixtures were prepared modifying the general protocol reported in the literature [ 15 ] and adjusting the dilution of monomers to avoid formation of unwanted lumps of polymer.…”

“…The eluates were lyophilized, weighed and stored at 4 °C. Size distribution and polydispersity index were determined as previously reported [ 22 ].…”

“…NanoMIPs and nanoNIPs were grafted onto aminated glass beads in accordance with the protocol previously reported [ 22 ].…”

“…This template was chosen as a system model as imprinted nanoparticles have been extensively reported in the literature as synthetic receptors in sensing devices [ 19 , 20 ] and solid-phase extraction [ 21 ]. Moreover, ciprofloxacin is strongly fluorescent, so it can be detected at very low concentrations by HPLC, making it possible to determine the binding isotherms in conditions of high dilution required by the equilibrium constants typical of nanoMIPs prepared by solid-phase synthesis [ 22 ]. The binding properties were studied by partition equilibrium and rebind kinetic experiments to measure the binding affinity and the kinetic rate constants.…”