Effect of exogenous testosterone on oxidative status of the testes in adult male rats

Abstract

The aim of this study was to investigate the testosterone-induced changes in the oxidative status of testes in adult male rats treated either with testosterone or after blockade of androgen receptors with cyproterone acetate. A total of 40 intact rats were divided into four groups: a control group receiving sterile oil, the testosterone group receiving testosterone isobutyras, the cyproterone group receiving cyproterone acetate and the combination group receiving both testosterone isobutyras and cyproterone acetate. Treatments were carried out for 2 days by intramuscular application. Parameters of oxidative stress and the expression levels of the steroidogenic acute regulatory protein (StAR) gene were measured in testes. Significantly increased TBARS and advanced glycation end products (AGEs) levels were found in the testosterone group when compared to the control group. The °1 ferric-reducing ability of the tissue and total antioxidative capacity were lower in the testosterone group in comparison with the control group. Gene expression analysis revealed significant downregulation of the StAR gene in the testes of rats in the testosterone and combination groups with respect to control animals. In conclusion, administration of exogenous testosterone influences the lipid peroxidation and carbonyl stress and decreases the antioxidant defence in the testes. These data might have implications for male fertility in humans.

The efficacy of antioxidant therapy against oxidative stress and androgen rise in ethylene glycol induced nephrolithiasis in Wistar rats

“…[58][59][60] It is reported that administration of exogenous testosterone influences the lipid peroxidation and carbonyl stress and decreases the antioxidant defense in the testes. 61 Moreover, supplementation of vitamin E (a-tocopherol) and 17-estradiol (E2) in ovariectomized diabetic rats resulted in an increase in antioxidant enzymes, Vit. A, and -carotene levels but a decrease in MDA levels played a role in preventing renal disorders.…”

Effect of Korean Herbal Formula (Modified Ojayeonjonghwan) on Androgen Receptor Expression in an Aging Rat Model of Late Onset Hypogonadism

“…Despite the efficacy of TRT in aging men with symptomatic LOH, the possibility of treatment-related adverse effects on cardiovascular disease, prostate cancer, and breast cancer remains uncertain. In addition, Tóthová et al [30] reported that exogenous T increases the oxidative stress and decreases anti-oxidative status in testes. In this respect, natural products or herbs (traditional herbal medicine) are considered a viable alternative treatment for LOH, without the adverse effects of TRT.…”

Oxidative Stress Induction in Monosex Nile Tilapia (Oreochromis niloticus, Linnaeus, 1758): A Field Study on the Side Effects of Methyltestosterone

“…In accordance, brown trout (Salmo trutta, L.) with higher testosterone levels prior to spawning have higher levels of oxidative damage exemplified by decreased antioxidant capacity at the time of spawning. In addition, testosterone induced changes in the oxidative status of rat testis by reducing TAC [32]. Enhanced reactive oxygen species production, and depleted antioxidant enzyme levels and activities are linked to testosterone administration in several published articles [33][34][35].…”