Effect of exercise on lipoprotein lipase activity in rat heart and skeletal muscle

Borensztajn, Jayme; Rone,; Babirak, SP; McGarr, JA; Lb, Oscai

doi:10.1152/ajplegacy.1975.229.2.394

Cited by 89 publications

(31 citation statements)

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“…Of interest, however, the Pima Indians in this study tended to have an increase rather than decrease in SMLPL after insulin administration. Perhaps this response helps to meet the fuel need ofmuscle in the setting ofinsulin resistance (31 Since a reduced proportion oftype I, high-oxidative muscle fibers has been implicated in the etiology of obesity ( 14) and both animal and human studies have established the relationship between muscle fiber type and muscle LPL activity (15)(16)(17)(18), SMLPL activity would be predicted to be low before or during the development ofthe obese state. In the study ofTaskinen et al (32), SMLPL was lower in obese subjects, whereas Reitman et al (33) found no difference between lean and obese.…”

Section: Resultsmentioning

confidence: 99%

“…Wade et al ( 14) showed that the combustion of FA during light exercise is related to muscle fiber-type proportions. It is also recognized, from studies in both humans and animals, that muscle LPL activity is higher in muscles composed predominantly of high-oxidative slow twitch fibers and low in muscle with greater content of low-oxidative fast twitch fibers (15)(16)(17)(18). We hypothesized that a high 24-h RQ, i.e., a low fat/CHO oxidation ratio, is associated with low skeletal muscle LPL activity.…”

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confidence: 99%

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Relationship between skeletal muscle lipoprotein lipase activity and 24-hour macronutrient oxidation.

Ferraro¹,

Eckel²,

Larson³

et al. 1993

J. Clin. Invest.

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A low ratio of whole-body 24-h fat/carbohydrate (CHO) oxidation has been shown to be a predictor of subsequent body weight gain. We tested the hypothesis that the variability of this ratio may be related to differences in skeletal muscle metabolism. Since lipoprotein lipase (LPL) plays a pivotal role in partitioning lipoprotein-borne triglycerides to adipose (storage) and skeletal muscle (mostly oxidation), we postulated that a low ratio of fat/CHO oxidation was associated with a low skeletal muscle LPL (SMLPL) activity. As an index of substrate oxidation, 24-h RQ was measured under sedentary and eucaloric conditions in 16 healthy nondiabetic Pima males. During a 6-h euglycemic, hyperinsulinemic clamp, muscle biopsies were obtained at baseline, 3, and 6 h. Heparin-elutable SMLPL activity was 2.92±0.56 nmol free fatty acids/g-min (mean±SD) at baseline, was unchanged (2.91±0.51) at the third hour, and increased significantly (P < 0.05) to 3.13±0.57 at the sixth hour of the clamp. The mean (of baseline and 3-h) SMLPL activity correlated inversely with 24-h RQ (r = 0.57, P < 0.03) but not with body size, body composition, or insulinmediated glucose uptake. Since SMLPL activity is related to the ratio of whole body fat/CHO oxidation rate, a decreased muscle LPL activity may, therefore, predispose to obesity. (J. Clin. Invest. 1993. 92:441-445.) Key words: lipoprotein lipase

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Relationship between skeletal muscle lipoprotein lipase activity and 24-hour macronutrient oxidation.

Ferraro¹,

Eckel²,

Larson³

et al. 1993

J. Clin. Invest.

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“…In animals, the results have been inconsistent. Borensztajn et al (23) found a significant increase of LPL activity with exercise in tissue homogenates of all fiber types of skeletal muscle. In contrast, other investigators were unable to find any difference in LPL activity of either muscle or adipose tissue between trained and sedentary rats (24)(25)(26).…”

Section: Discussionmentioning

confidence: 97%

The regulation of adipose tissue and muscle lipoprotein lipase in runners by detraining.

Simsolo¹,

Ong²,

Kern³

1993

J. Clin. Invest.

101

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IntroductionTo study the mechanism of lipoprotein lipase (LPL) regulation by exercise, we recruited 16 healthy athletes to undergo a 2-wk period of detraining. Fasting fat and muscle biopsies were performed both before and after the detraining period. In muscle, detraining resulted in a decrease in LPL activity in both the heparin-releasable (HR) (-45%, P < 0.05) and cellular (ex- Triglyceride-derived FFA are taken up by tissues through the action oflipoprotein lipase (LPL)'(1), which is found predominantly in adipose tissue and muscle. In human adipose tissue, little FFA is synthesized from glucose, and therefore LPL is an important enzyme for lipid storage (2). In contrast, muscle uses FFA for oxidation (3). The LPL found in both adipose tissue and muscle contributes to the clearance of circulating triglyceride-rich lipoproteins.Previous studies have examined the effects of exercise on LPL activity, using a number of different exercise regimens (reviewed in reference 4). Studies of endurance training in human subjects showed significant increases in LPL activity, both in skeletal muscle and adipose tissue, as well as in postheparin plasma. Weight loss also yields increases in adipose LPL (5), however, and many studies of exercise did not control for weight loss during the exercise regimen. Kiens et al. (6) studied six healthy men in which LPL activity was measured in the knee extensors at rest and after 8 wk of training of one leg, while the other leg was not exercised and served as a control. They found a 70% increase in LPL activity in the trained leg compared with the nontrained one.A number of previous studies have examined the mechanism of LPL regulation, and have discovered that LPL may be regulated at multiple different cellular sites (7). This study was designed to examine the mechanism of regulation of LPL by exercise in adipose tissue and muscle. As described below, short-term changes in exercise altered LPL activity in both tissues through posttranslational changes. Methods Recruitment ofstudy subjects. 16 subjects (10 men and 6 women; age, 37±2 yr) were recruited for this study, and their characteristics are shown in Table I. All subjects were in good health, were taking no medications, and were running 2 32 km/wk, or performing an equivalent amount of aerobic exercise (one subject bicycled -600 km/ week). 6 of the 16 subjects ran a marathon within 1 mo preceding the study. To participate in the study, the subjects had to agree to detrain for 2 wk, and gave informed consent to the procedures. Detraining entailed no aerobic exercise, but just activities of daily living. Compliance with these instructions was judged to be excellent. This protocol was approved by the Internal Review Board of Cedars-Sinai Medical Center, and subjects gave informed consent to the procedures.

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“…feeding/ fasting [4], cold adaptation [5], physical exercise [6,7], lactation [8] and infections/tumours [9]. Hormones involved in the regulation include insulin, catecholamines, glucocorticoids and tumour necrosis factor (reviewed in refs.…”

Section: Introductionmentioning

confidence: 99%

Two different mechanisms are involved in nutritional regulation of lipoprotein lipase in guinea-pig adipose tissue

Semb

Olivecrona

1989

Biochemical Journal

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Lipoprotein lipase activity in adipose tissue responds rapidly to changes in the physiological state. To study what mechanisms are involved in the regulation, guinea pigs were fasted and the decrease in adipose-tissue lipoprotein lipase activity was compared with the decreases in mRNA and lipase synthesis. The mRNA pattern (three species) did not change. There was a close parallelism between the abundance of lipase mRNA and relative lipase synthesis (immunoprecipitable 35S-labelled lipoprotein lipase as fraction of total [35S]protein after pulse-labelling with [35S]methionine). Total protein synthesis decreased on fasting, compounding the decrease in relative lipase synthesis. Lipoprotein lipase mRNA changed similarly in fatpads and in isolated adipocytes, whereas lipase activity changed more in the pads, indicating disproportionally large changes in extracellularly located lipase. In old guinea pigs the decreases in lipoprotein lipase activity and lipase synthesis were comparable, but in young animals the change in lipase activity was substantially larger than the change in lipase synthesis. Refeeding of fasted young guinea pigs with glucose resulted in a rapid increase in lipoprotein lipase activity, but there was only a small change in lipase mRNA. Old animals responded slowly to refeeding. The results indicate that in older animals the major mechanism for regulation of adipose. lipoprotein lipase activity is a relatively slow change in lipase mRNA, whereas in younger animals an additional, more rapid, regulation is exerted on the transport and turnover of the enzyme.

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Effect of exercise on lipoprotein lipase activity in rat heart and skeletal muscle

Cited by 89 publications

References 0 publications

Relationship between skeletal muscle lipoprotein lipase activity and 24-hour macronutrient oxidation.

Relationship between skeletal muscle lipoprotein lipase activity and 24-hour macronutrient oxidation.

The regulation of adipose tissue and muscle lipoprotein lipase in runners by detraining.

Two different mechanisms are involved in nutritional regulation of lipoprotein lipase in guinea-pig adipose tissue

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