Effect of ethylthiosulfаnylate and Chrome(VI) on the pro/antioxidant system in rats blood

Kotyk, B. І.; Iskra, R.; Sushko, O.; Slivinska, O. M.; Klymets, G.; Buchko, O.; Pylypets, A. Z.; Pryimych, V. I.

doi:10.15407/animbiol21.04.038

Cited by 5 publications

(1 citation statement)

References 66 publications

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“…The absorption value was measured after adding 0.2 ml of 20% ammonium thiocyanate solution at λ 480 nm. The content of lipid hydroperoxides was determined by the difference between the experimental sample and the control, but instead of the homogenate, 0.2 ml of bidistilled water was added, expressed in conditional units per 1 g of tissue [30].…”

Section: Methodsmentioning

confidence: 99%

Prooxidant-antioxidant profile in tissues of rats under the action of thiosulfonate esters

Liubas¹,

Iskra²,

Kotyk³

et al. 2023

Ukr.Biochem.J.

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Thiosulfonates are characterized by a wide spectrum of biological activity and have effective antimicrobial, antithrombotic, antitumor and antiparasitic effects. However, the use of synthetic sulfur-containing compounds for therapeutic and preventive purposes requires the study of their effect on the protective mechanisms of maintaining homeostasis, the antioxidant status of the body in particular. The aim of the study was to estimate lipid peroxidation process and the state of the antioxidant system in the kidneys, spleen, brain, and muscles of rats under the influence of newly synthesized sulfur-containing compounds of S-alkyl esters of thiosulfonic acids S-ethyl-4-aminobenzenethiosulfonate (ETS), S-allyl-4-aminobenzenethiosulfonate (ATS) and S-allyl-4-acetylaminobenzenethiosulfonate (AATS). Male Wistar rats kept on a standard diet were divided into four groups of 5 animals each: I (control), II, III, IV (experimental). The animals of the control group received additional 0.5 ml of oil and the animals of the experimental groups 0.5 ml of oil solution of thiosulfonate esters once a day. The animals of II, III and IV groups were given ETS, ATS, AATS at the rate of 100 mg per kg of body weight. The lipid hydroperoxides (LPH), TBA-active products, GSH content and activity of SOD, catalase, glutathione peroxidase, glutathione reductase in tissue homogenates were determined. The multidirectional changes of the studied indicators after consumption of S-alkyl esters of thiosulfonic acids for 21 days have been identified and characterized indicating on different degree of inhibition or activation of LPO processes depending on the type of tissue and the structure of thiosulfonate ester. Keywords: antioxidant system, rat tissues, S-allyl-4-acetylaminobenzenethiosulfonate, S-allyl-4-aminobenzenethiosulfonate, S-ethyl-4-aminobenzenethiosulfonate

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Section: Methodsmentioning

confidence: 99%

Prooxidant-antioxidant profile in tissues of rats under the action of thiosulfonate esters

Liubas¹,

Iskra²,

Kotyk³

et al. 2023

Ukr.Biochem.J.

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Effect of ethylthiosulfаnylate in combination with vitamin E on certain biochemical blood parameters and hematological indicators in rats under the influence of Cr(VI)

Kotyk¹

2023

Bìol. Tvarin

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The aim of our study was to investigate the effect of ethylthiosulfаnylate, a representative of the class of thiosulfonate compounds, in combination with vitamin E on certain biochemical blood parameters, hematological indicators and total Chromium content in liver of rats exposed to Cr(VI). Laboratory rats were divided into 8 groups of 5 animals each. Animals of group I (intact control) were injected daily intraperitoneally with 150 μl of physiological saline solution for 7 days. Rats of III/IV groups received intraperitoneal daily administration of K2Cr2O7 (diluted in physiological saline solution at a dose of 2.5 mg Cr(VI)/kg) for 7/14 days. Animals of the II experimental group were injected daily intragastrically with 1000 μl of sunflower oil for 14 days. Rats of V/VI experimental groups were administrated daily intragastrically with 1000 μl of an oil solution of vitamin E (20 mg/kg)/vitamin E (20 mg/kg) in combination with ethylthiosulfаnylate (100 mg/kg) for 14 days. Animals of VII/VIII experimental groups were injected daily intragastrically with 1000 μl of an oil solution of vitamin E (20 mg/kg) in combination with ethylthiosulfаnylate (100 mg/kg) for 14 days, after which a 7-day/14-day period of intraperitoneal K2Cr2O7 administration was performed. Exposure to Cr(VI) led to a decrease in the number of erythrocytes, leukocytes, content of hemoglobin, phospholipids, total protein against the background of the accumulation of total lipids, mono- and diglycerides, non-esterified fatty acids, and creatinine in blood of rats. Chromium concentration significantly increased in the liver of rats after administration of Cr(VI). The combined effect of vitamin E and ethylthiosulfаnylate contributed to the partial compensation of Cr(VI)-induced disturbances of the number of leukocytes and content of total proteins, phospholipids, non-esterified fatty acids in blood of rats. Vitamin E and ethylthiosulfаnylate pretreatment also contributed to the reduction of the percentage accumulation of Chromium in liver of rats injected with Cr(VI).

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Influence of liposomal thiosulfonate drug on the blood parameters of cows suffering catarrhal mastitis

Suprovych,

Stroianovska,

Vishchur

et al. 2023

Regul. Mech. Biosyst.

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Diseases of the mammary gland in cows are of multi-factor nature, which significantly complicates their control and treatment. One of the factors influencing development of mastitis is bacterial microflora. Because of limited use of antibiotics, development of novel alternative antmicrobial drugs is of great relevance. Therefore, the objective of the study was identifying the effect of an ethyl-thiosulfanilate-based liposomal drug on the hematological and biochemical parameters of blood of the cows suffering catarrhal mastitis. The animals were three times intramuscularly injected with the liposomal drug in the dose of 0.04 mL/kg of body weight. Blood from the cows was taken from the jugular vein on the day prior to administration of the drug, and also on the 3rd and 7th days after its application. During the bacterial study of the secretion of udder of sick cows, we collected 51 bacterial isolates, in which dominated Staphylococcus aureus (27.5%) and Streptococcus spp. (21.6%). In blood of the sick cows, we found higher number of leukocytes, increases in the concentrations of circulating immune complexes, lipid hydroperoxides, and ketone derivatives of oxidative modification of proteins. At the same time, we observed decreases in the number of lymphocytes and concentration of protein and the bactericidal activity of blood serum. Administration of the complex liposomal drug had a normalizing effect on the analyzed parameters of the cows. This was evidenced by 9.4% decrease in the total number of leukocytes, 18.5% decrease in the content of circulating immune complexes, 9.3% increase in lymphocytes, and 13.6% increase in the level of total protein. In the sick cows, the drug enhanced the bactericidal (by 35.4%) and lysozymic (by 36.3%) activities of blood serum, glutathione peroxidase activity, and increased the content of reduced glutathione. On the seventh day after injecting the drug into blood of the cows, we saw decrease in the level of intermediate and end products of lipid peroxidation and derivatives of oxidative modification of proteins. Therefore, the complex ethyl-thiosulfanilate-based liposomal drug promoted reduction of metabolic homeostasis of the organism, had positive effect on the activity of natural defense mechanisms in the organism and can be recommended for the treatment of cows with catarrhal mastitis.

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Effect of ethylthiosulfаnylate and Chrome(VI) on the pro/antioxidant system in rats blood

Cited by 5 publications

References 66 publications

Prooxidant-antioxidant profile in tissues of rats under the action of thiosulfonate esters

Prooxidant-antioxidant profile in tissues of rats under the action of thiosulfonate esters

Effect of ethylthiosulfаnylate in combination with vitamin E on certain biochemical blood parameters and hematological indicators in rats under the influence of Cr(VI)

Influence of liposomal thiosulfonate drug on the blood parameters of cows suffering catarrhal mastitis

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