Effect of ethylthiosulfаnylate in combination with vitamin E on certain biochemical blood parameters and hematological indicators in rats under the influence of Cr(VI)

Abstract

The aim of our study was to investigate the effect of ethylthiosulfаnylate, a representative of the class of thiosulfonate compounds, in combination with vitamin E on certain biochemical blood parameters, hematological indicators and total Chromium content in liver of rats exposed to Cr(VI). Laboratory rats were divided into 8 groups of 5 animals each. Animals of group I (intact control) were injected daily intraperitoneally with 150 μl of physiological saline solution for 7 days. Rats of III/IV groups received intraperitoneal daily administration of K2Cr2O7 (diluted in physiological saline solution at a dose of 2.5 mg Cr(VI)/kg) for 7/14 days. Animals of the II experimental group were injected daily intragastrically with 1000 μl of sunflower oil for 14 days. Rats of V/VI experimental groups were administrated daily intragastrically with 1000 μl of an oil solution of vitamin E (20 mg/kg)/vitamin E (20 mg/kg) in combination with ethylthiosulfаnylate (100 mg/kg) for 14 days. Animals of VII/VIII experimental groups were injected daily intragastrically with 1000 μl of an oil solution of vitamin E (20 mg/kg) in combination with ethylthiosulfаnylate (100 mg/kg) for 14 days, after which a 7-day/14-day period of intraperitoneal K2Cr2O7 administration was performed. Exposure to Cr(VI) led to a decrease in the number of erythrocytes, leukocytes, content of hemoglobin, phospholipids, total protein against the background of the accumulation of total lipids, mono- and diglycerides, non-esterified fatty acids, and creatinine in blood of rats. Chromium concentration significantly increased in the liver of rats after administration of Cr(VI). The combined effect of vitamin E and ethylthiosulfаnylate contributed to the partial compensation of Cr(VI)-induced disturbances of the number of leukocytes and content of total proteins, phospholipids, non-esterified fatty acids in blood of rats. Vitamin E and ethylthiosulfаnylate pretreatment also contributed to the reduction of the percentage accumulation of Chromium in liver of rats injected with Cr(VI).