Effect of Ethephon, Indole Butyric Acid, and Treatment Solution pH on Rooting and on Ethylene Levels within Mung Bean Cuttings

Mudge, Kenneth W.; Swanson, Bert T.

doi:10.1104/pp.61.2.271

Cited by 33 publications

(15 citation statements)

References 9 publications

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“…Results of these studies have been highly variable. For example, in experiments using applied ethylene or ethylene-generating compounds to examine adventitious rooting in mung bean, researchers showed that ethylene stimulates rooting (Robbins et al, 1983), inhibits rooting (Geneve and Heuser, 1983), or has no effect (Mudge and Swanson, 1978). Similar experiments in tomato (Lycopersicon esculentum) have shown stimulatory effects (Hitchcock and Zimmerman, 1940;Orion and Minz, 1969;Phatak et al, 1981) and inhibitory effects (Roy et al, 1972;Coleman et al, 1980).…”

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Root Formation in Ethylene-Insensitive Plants

Clark¹,

Gubrium²,

Barrett³

et al. 1999

Plant Physiology

200

133

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Experiments with ethylene-insensitive tomato (Lycopersicon esculentum) and petunia (Petunia x hybrida) plants were conducted to determine if normal or adventitious root formation is affected by ethylene insensitivity. Ethylene-insensitive Never ripe (NR) tomato plants produced more below-ground root mass but fewer above-ground adventitious roots than wild-type Pearson plants. Applied auxin (indole-3-butyric acid) increased adventitious root formation on vegetative stem cuttings of wild-type plants but had little or no effect on rooting of NR plants. Reduced adventitious root formation was also observed in ethylene-insensitive transgenic petunia plants. Applied 1-aminocyclopropane-1-carboxylic acid increased adventitious root formation on vegetative stem cuttings from NR and wild-type plants, but NR cuttings produced fewer adventitious roots than wild-type cuttings. These data suggest that the promotive effect of auxin on adventitious rooting is influenced by ethylene responsiveness. Seedling root growth of tomato in response to mechanical impedance was also influenced by ethylene sensitivity. Ninety-six percent of wild-type seedlings germinated and grown on sand for 7 d grew normal roots into the medium, whereas 47% of NR seedlings displayed elongated tap-roots, shortened hypocotyls, and did not penetrate the medium. These data indicate that ethylene has a critical role in various responses of roots to environmental stimuli.

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confidence: 99%

Root Formation in Ethylene-Insensitive Plants

Clark¹,

Gubrium²,

Barrett³

et al. 1999

Plant Physiology

200

133

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“…Although the results of these studies have been quite variable (Geneve and Heuser 1983, Johnson and Hamilton 1977, Lipecki and Selwa 1973, Swanson 1976, most indicate that ethephon either promotes rooting (Carpenter 1975, Krishnamoorthy 1971, Robbins et al 1983b, Roy et al 1972 or has no influence on it (Chong 1982, Criley and Parvin 1979, Mudge and Swanson 1978, Nell and Sanderson 1972, Samananda et al 1972, Sanderson and Patterson 1980, Schier 1975. This variable response to ethylene could be expected, as a large number of species at different stages of development have been tested under a range of experimental conditions.…”

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confidence: 99%

“…Furthermore, ethephon may have extraneous effects on rooting which make interpretation of results difficult. These effects include the acidity of unbuffered so-lutions (Reid et al 1980), the pH dependency of release rate (Mudge and Swanson 1978), and the possible influence of breakdown products on rooting results.…”

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The effect of ethylene on adventitious root formation in mung bean (Vigna radiata) cuttings

Robbins

Reid

Paul

et al. 1985

J Plant Growth Regul

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Abstract.A promotive effect of ethylene on the formation of adventitious roots by mung bean cuttings was demonstrated using a recirculating solution culture system to apply dissolved ethylene. The number of roots increased in proportion to the length of exposure to the gas. Mean root numbers per cutting for a 4-day exposure to ethylene and an air control were 45 and 19, respectively. The tissue was most sensitive to a 24-h ethylene '~pulse" 2-3 days after taking cuttings. Rooting was maximal at a concentration of 13 t~1 1-1 ethylene. The ethylene treatment inhibited the growth of roots and terminal buds. Application of Ag § , as silver thiosulfate, reversed the effect of ethylene on the two growth responses but had no effect on root numbers. Norbornadiene, another inhibitor of ethylene action, reversed all three ethylene responses.Numerous workers have used the ethylene-releasing compound (2-chloroethyl) phosphonic acid (ethephon) to examine the effect of ethylene on the rooting of cuttings. Although the results of these studies have been quite variable

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“…The fact that ethylene may stimulate [13,28], inhibit [5,12], or be without effect on rooting [17] has caused some confusion in this research area . The possibility that auxininduced ethylene is involved in the auxinstimulated rooting has also been considered in this literature, although the conclusions differ [10,18,22] .…”

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Interaction of ethylene with indole-3-acetic acid in regulation of rooting in pea cuttings

Nordström

Eliasson

1993

Plant Growth Regul

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Cuttings of pea (Pisum sativum L . cv Marma) were treated with 1-aminocyclopropane-l-carboxylic acid (ACC) . This treatment caused increased ethylene production and reduction of root formation . The effect of 0 .1 mM ACC on the level of endogenous indole-3-acetic acid (IAA) in the rooting zone and in the shoot apex was analyzed by gas chromatography-single ion monitoring mass spectrometry or by high pressure liquid chromatography with fluorimetric detection (HPLC) . Concentrations of indole-3-acetylaspartic acid (IAAsp) in the stem bases were also determined using HPLC . The ACC treatment had little effect on the IAA level in the base measured after 24 h, but caused a considerable decrease during the 3 following days . IAAsp increased in the base on days 1, 2 and 3 and then declined . The build up of IAAsp in the base was not affected by ACC during the first two days of the treatment, but later this conjugate decreased more rapidly than in controls . No effect of the ACC treatment was found on the level of IAA in the apex . IAA (1 EiM) applied to the cuttings during 24 h reduced the number of roots formed . The possibility that IAA-induced ethylene is involved in this response was investigated .Our results support earlier evidence that the inhibitory effect of ethylene on rooting in pea cuttings is due to decreased IAA levels in the rooting zone . The inhibitory effect of applied IAA is obtained if the internal IAA level is maintained high during the first 24 h, whereas stimulation of rooting occurs if the internal IAA level remains high during an extended period of time . Our results do not support the suggestion that ethylene mediates the inhibitory effect of applied IAA .

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Effect of Ethephon, Indole Butyric Acid, and Treatment Solution pH on Rooting and on Ethylene Levels within Mung Bean Cuttings

Cited by 33 publications

References 9 publications

Root Formation in Ethylene-Insensitive Plants

Root Formation in Ethylene-Insensitive Plants

The effect of ethylene on adventitious root formation in mung bean (Vigna radiata) cuttings

Interaction of ethylene with indole-3-acetic acid in regulation of rooting in pea cuttings

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