Effect of Ethanol Extracts of Three Chinese Medicinal Plants With Anti-diarrheal Properties on Ion Transport of the Rat Intestinal Epithelia

Abstract

Abstract. Effects of ethanol extracts of three Chinese medicinal plants, namely, Qinpi (Fraxini cortex), Kushen (Sophora flavescens, AITON), and Huanglian (Coptis teeta, WALLICH), on ion transport of the rat intestinal epithelia were determined in this study. Rat intestinal epithelia mounted in an Ussing chamber attached to a voltage / current clamp were used for measuring changes in the short circuit current across the epithelia. Activation of the intestinal epithelia by serosal administration of 5 m M forskolin resulted in an increase in basal short circuit current. The ethanol extracts of each of the three plants partially reduced the current stimulated by forskolin. In the following experiments, ouabain and bumetanide were added prior to adding the ethanol extract of these plants for revealing their effect on Na + and Cl -movement. The results suggest that the ethanol extract of the Qinpi would affect Cl -transport. On the contrary, the ethanol extract of Kushen would affect Na + transport rather than Cl -movement. This study provides evidences that reveal the pharmacological mechanism of the Chinese plants with anti-diarrheal properties.

The mechanism of vasorelaxation induced by ethanol extract of Sophora flavescens in rat aorta

“…Sophora flavescens has been used for the treatment of diarrhea and arrhythmia in China (Dai et al, 1986;Tsai et al, 2004). In the current study, we found ESF caused acute relaxation of isolated aortic rings via endothelium-dependent NO-cGMP signaling.…”

“…Sophora flavescens AIT (Leguminosae) is a shrub distributed in China, Japan and some European countries and has been used as a folk medicine for various diseases, including diarrhea and arrhythmia (Dai et al, 1986;Tsai et al, 2004). Previously, it was reported that Sophora flavescens root contains alkaloids, triterpenoids, and flavonoids (Tang and Eisenbrand, 1992;Jung et al, 2005), some of which have shown various biological activities such as antiinflammatory, antioxidant, and anti-diabetic effects Jung et al, 2005Jung et al, , 2006.…”

Antibacterial activity of sophoraflavanone G Isolated from the roots of Sophora flavescens against methicillin‐resistant Staphylococcus aureus

“…Sophorae radix, the dried root of Sophora flavescens AITON (Leguminosae), is an important herbal medicine, which has been traditionally employed as an antipyretic, analgesic, antihelminthic, and stomachic, and has been shown to exert antibacterial, anti-inflammatory, antioxidant, and antimalarial effects (Kuroyanagi et al, 1999;Hsiang et al, 2001;Tsai et al, 2004;. S. flavescens is also known to harbor numerous flavonoids, including formononetin, kushenol E, kushenol B, sophoraflavanone G, kushenol L, kushenol M, kuraridin, kurarinone, kushenol N, and kushenol F (Ryu et al, 1960;Hirobumi et al, 2002;Kang et al, 2000).…”

Simultaneous analysis of coumarins and secoiridoids in Cortex Fraxini by high-performance liquid chromatography–diode array detection–electrospray ionization tandem mass spectrometry

“…The drug is mainly used as an antibacterial, analgesic and anti-inflammatory agent for the treatment of diarrhea, bacillary dysentery, arthritis and hyperuricemia [2][3][4][5]. Other pharmaco- * Corresponding author.…”