Effect of Ethamsylate on Carrageenan‐induced Rat Paw Oedema: A Comparison With Indomethacin

Gard, Paul R.; Trigger, D. J.

doi:10.1111/j.1440-1681.1990.tb01285.x

Cited by 7 publications

(4 citation statements)

References 12 publications

(10 reference statements)

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“…This is due to the fact that inflammatory reaction is very complex so anti-inflammatory activity can be appreciated by different manners, the interpretation varies according to the test used. For example, indomethacin inhibits carrageenan induced paw edema (Gard and Trigger, 1990) …”

Section: Discussionmentioning

confidence: 99%

In vitro effects of aqueous seeds extract of Acacia cyanophylla on the opsonized zymosan-induced superoxide anions production by rat polymorphonuclear leukocytes

Abbouyi¹,

Toumi²,

Hachimi³

et al. 2004

Journal of Ethnopharmacology

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Section: Discussionmentioning

confidence: 99%

In vitro effects of aqueous seeds extract of Acacia cyanophylla on the opsonized zymosan-induced superoxide anions production by rat polymorphonuclear leukocytes

Abbouyi¹,

Toumi²,

Hachimi³

et al. 2004

Journal of Ethnopharmacology

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“…5 Ethamsylate appears to ensure haemostasis by increasing platelet-leukocyte aggregates. 6 The drug also exhibited other important pharmacological actions, scavenging hydroxyl radicals, 7 inhibiting prostaglandin synthesis, 8 decreasing inflammation 9 and decreasing vascular peritoneal permeabilization due to arachidonate. 7 In light of the above data and in view of the wide use of ethamsylate to control capillary bleeding, it looked pertinent to investigate the effect of the drug on the development of liver injury.…”

Section: Ethamsylatementioning

confidence: 99%

Untitled

2017

JDMDC

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This industrial solvent is widely used to cause acute hepatic injury with centrilobular necrosis in rodents. Moreover, its long-term administration is associated with the development of liver cirrhosis and ascites. It is thus used to investigate potential therapeutic agents. 10 The development of liver injury is largely ascribed to the formation of the trichloromethyl radical, CCl 3 and the ensuing oxidative cellular damage, 11 although vascular, 12 and inflammatory 13 mechanisms are also involved. Materials and method Animals Adult Sprague-Dawley rats of either sex, weighing 130-150g were used in this study. Rats were fed with standard laboratory chow and water Ad libitum. Animal procedures were performed in accordance with the Ethics Committee of the National Research Centre and followed the recommendations of the National Institutes of Health Guide for Care and Use of Laboratory Animals (Publication No. 85-23, revised 1985). Drugs and chemicals Carbon tetrachloride (CCl 4) (BDH Chemicals, UK), and ethamsylate (MINAPHARM Pharmaceuticals, Egypt) were used. The doses for rats used were based upon the human dose after conversion to that of rat according to Paget and Barnes conversion tables. 14 The CCl 4 model of acute hepatic damage The rats were divided into 5 equal groups (six rats each). Groups 1-4 received CCl 4 in olive oil (1:1, vol/vol) at a dose of 2.8ml/kg by gavage. Starting on the first day of CCl 4 , rats were also treated with ethamsylate (45, 90 or 180mg/kg) once daily orally for one week. The fifth group of rats was treated with the vehicle (olive oil) at 2.8ml/kg (no CCl 4). Rats had free access to food and drinking water during the study. Biochemical assessment At the end of the study, blood samples were obtained from the retro-orbital venous plexus, under light ether anaesthesia. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) activities in serum were measured using commercially available kits (Biodiagnostic, Egypt). Histopathology After the end of the treatment period, rats were killed, the liver of different groups was removed, fixed in 10% formalin and 5µm thick paraffin sections were stained with haematoxylin and eosin (H&E) and examined under the light microscope. Statistical analysis Data are expressed as means±SE. Multiple group comparisons were performed by ANOVA followed by Duncan's multiple range test P<0.05 was considered statistically significant. Results Effect of ethamsylate on serum enzyme activities Rats treated with CCl 4 exhibited markedly increased activities of

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“…66 The antioxidant activity of ethamsylate can explain its reduction of prostaglandin biosynthesis independent of cyclooxygenase 16 and the inhibition of carrageenan-induced rat paw edema. 61 Therefore, we recently tested ethamsylate in a rat peritoneal model of vascular permeabilization by arachidonic acid, a model in which arachidonic responses were potently antagonized by antioxidants. 66,67 Intravenous ethamsylate was found to potently antagonize the peritoneal vascular permeabilization induced by arachidonate in this rat peritoneal model.…”

Section: Antioxidant/antiinflammatory Actionsmentioning

confidence: 99%

Therapeutic Efficacy and Mechanism of Action of Ethamsylate, a Long-Standing Hemostatic Agent

Garay¹,

Chiavaroli²,

Hannaert

2006

American Journal of Therapeutics

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Ethamsylate (2,5-dihydroxy-benzene-sulfonate diethylammonium salt) is a synthetic hemostatic drug indicated in cases of capillary bleeding. This review covers more than 40 years of intensive clinical and fundamental research with ethamsylate. First, we summarize the large medical literature concerning its clinical efficacy. Of these, well-controlled clinical trials clearly showed the therapeutic efficacy of ethamsylate in dysfunctional uterine bleeding, with the magnitude of blood-loss reduction being directly proportional to the severity of the menorrhagia. Other well-controlled clinical trials showed therapeutic efficacy of ethamsylate in periventricular hemorrhage in very low birth weight babies and surgical or postsurgical capillary bleeding. Second, we review the numerous investigations performed to elucidate the mechanism of action of ethamsylate. Ethamsylate acts on the first step of hemostasis by improving platelet adhesiveness and restoring capillary resistance. Recent studies showed that ethamsylate promotes P-selectin-dependent, platelet adhesive mechanisms. Finally, we compare ethamsylate with other recent hemostatic agents. It is suggested that the place of ethamsylate as a hemostatic agent is that of a mild but well-tolerated drug, particularly useful in dysfunctional uterine bleeding when contraception is not needed.

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Effect of Ethamsylate on Carrageenan‐induced Rat Paw Oedema: A Comparison With Indomethacin

Cited by 7 publications

References 12 publications

In vitro effects of aqueous seeds extract of Acacia cyanophylla on the opsonized zymosan-induced superoxide anions production by rat polymorphonuclear leukocytes

In vitro effects of aqueous seeds extract of Acacia cyanophylla on the opsonized zymosan-induced superoxide anions production by rat polymorphonuclear leukocytes

Untitled

Therapeutic Efficacy and Mechanism of Action of Ethamsylate, a Long-Standing Hemostatic Agent

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