Effect of Eriodictyol on Glucose Uptake and Insulin Resistance in Vitro

Zhang, Weiyun; Lee, Jung‐Jin; Kim, Young S.; Kim, In-Su; Han, Joo‐Hui; Lee, Sang-Gil; Ahn, Minwook; Jung, Sungyup; Myung, Chang‐Seon

doi:10.1021/jf300601z

Cited by 101 publications

(73 citation statements)

References 38 publications

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“…Both 300 µM propionic acid and 500 µM valeric acid increased significantly insulin-stimulated glucose uptake by 85.1% (Δ to insulin-treated) and 74.8%, respectively. A PPARγ agonist, rosiglitazone as a positive control [21]–[23], increased significantly both basal by 60.0% and insulin-stimulated glucose uptake by 170.3%. However, in the case of rosiglitazone, the increasing rate of glucose uptake merely by insulin was about 110.3% (Δ between insulin-stimulated and basal glucose uptake).…”

Section: Resultsmentioning

confidence: 99%

The Effects of Propionate and Valerate on Insulin Responsiveness for Glucose Uptake in 3T3-L1 Adipocytes and C2C12 Myotubes via G Protein-Coupled Receptor 41

et al. 2014

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Since insulin resistance can lead to hyperglycemia, improving glucose uptake into target tissues is critical for regulating blood glucose levels. Among the free fatty acid receptor (FFAR) family of G protein-coupled receptors, GPR41 is known to be the Gαi/o-coupled receptor for short-chain fatty acids (SCFAs) such as propionic acid (C3) and valeric acid (C5). This study aimed to investigate the role of GPR41 in modulating basal and insulin-stimulated glucose uptake in insulin-sensitive cells including adipocytes and skeletal muscle cells. Expression of GPR41 mRNA and protein was increased with maximal expression at differentiation day 8 for 3T3-L1 adipocytes and day 6 for C2C12 myotubes. GPR41 protein was also expressed in adipose tissues and skeletal muscle. After analyzing dose-response relationship, 300 µM propionic acid or 500 µM valeric acid for 30 min incubation was used for the measurement of glucose uptake. Both propionic acid and valeric acid increased insulin-stimulated glucose uptake in 3T3-L1 adipocyte, which did not occur in cells transfected with siRNA for GPR41 (siGPR41). In C2C12 myotubes, these SCFAs increased basal glucose uptake, but did not potentiate insulin-stimulated glucose uptake, and siGPR41 treatment reduced valerate-stimulated basal glucose uptake. Therefore, these findings indicate that GPR41 plays a role in insulin responsiveness enhanced by both propionic and valeric acids on glucose uptake in 3T3-L1 adipocytes and C2C12 myotubes, and in valerate-induced increase in basal glucose uptake in C2C12 myotubes.

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Section: Resultsmentioning

confidence: 99%

The Effects of Propionate and Valerate on Insulin Responsiveness for Glucose Uptake in 3T3-L1 Adipocytes and C2C12 Myotubes via G Protein-Coupled Receptor 41

et al. 2014

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“…10,18 Li et al 10 reported that eriodictyol elevated the activities of SOD, CAT, and GPx, as well as inhibited the production of malondialdehyde, thiobarbituric acid reactive substances (TBARS), TNF-α and IL-1β in kidney tissues. 19 Moreover, eriodictyol attenuates the degree of retinal inflammation and plasma lipid peroxidation, and preserves the blood-retinal barrier (BRB) integrity in streptozotocin-induced diabetic rats. In addition, eriodictyol increased insulin-induced glucose uptake in human hepatocellular liver carcinoma cells (HepG2) and differentiated 3T3-L1 adipocytes under HG condition.…”

Section: Discussionmentioning

confidence: 99%

Eriodictyol inhibits high glucose‐induced oxidative stress and inflammation in retinal ganglial cells

et al. 2018

J of Cellular Biochemistry

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Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes mellitus and is considered as a leading cause of blindness. Oxidative stress and inflammation are significant drivers for the development of DR. Eriodictyol, a flavonoid compound, was proved to possess anti‐inflammatory, antioxidative, and antidiabetic activities. However, the role of eriodictyol in DR has not been unveiled. In the current study, we explored the protective effects of eriodictyol on high glucose (HG)‐induced rat retinal ganglial cells (RGCs). The results suggested that eriodictyol improved cell viability of HG‐induced rat RGC‐5 cells in a dose‐dependent manner. Eriodictyol reduced the reactive oxygen species production and increased the activities of superoxide dismutase, glutathione peroxidase and catalase in rat RGC‐5 cells in response to HG stimulation. The production of proinflammatory cytokines including tumor necrosis factor alpha and interleukin‐8 was diminished after eriodictyol treatment. Eriodictyol also suppressed cell apoptosis induced HG in rat RGC‐5 cells. Furthermore, eriodictyol enhanced the nuclear translocation of nuclear factor erythroid‐2 (E2)‐related factor 2 (Nrf2) and elevated the expression of antioxidant enzyme heme‐oxygenase‐1 (HO‐1). These findings suggested that eriodictyol protects the RGC‐5 cells from HG‐induced oxidative stress, inflammation, and cell apoptosis through regulating the activation of Nrf2/HO‐1 pathway.

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“…Supplementation with eriodictyol suppressed diabetes with upregulation of mRNA expression of PPARγ2 and adipocyte-specific fatty acid-binding protein as well as the protein levels of PPARγ2 in differentiated 3T3-L1 adipocytes. Furthermore, eriodictyol reactivated Akt in HepG2 cells with HG-induced insulin resistance [103]. …”

Section: Natural Anti-diabetic/inflammatory Bioactive Compounds Anmentioning

confidence: 99%

Natural Phyto-Bioactive Compounds for the Treatment of Type 2 Diabetes: Inflammation as a Target

et al. 2016

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Diabetes is a metabolic, endocrine disorder which is characterized by hyperglycemia and glucose intolerance due to insulin resistance. Extensive research has confirmed that inflammation is closely involved in the pathogenesis of diabetes and its complications. Patients with diabetes display typical features of an inflammatory process characterized by the presence of cytokines, immune cell infiltration, impaired function and tissue destruction. Numerous anti-diabetic drugs are often prescribed to diabetic patients, to reduce the risk of diabetes through modulation of inflammation. However, those anti-diabetic drugs are often not successful as a result of side effects; therefore, researchers are searching for efficient natural therapeutic targets with less or no side effects. Natural products’ derived bioactive molecules have been proven to improve insulin resistance and associated complications through suppression of inflammatory signaling pathways. In this review article, we described the extraction, isolation and identification of bioactive compounds and its molecular mechanisms in the prevention of diabetes associated complications.

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Effect of Eriodictyol on Glucose Uptake and Insulin Resistance in Vitro

Cited by 101 publications

References 38 publications

The Effects of Propionate and Valerate on Insulin Responsiveness for Glucose Uptake in 3T3-L1 Adipocytes and C2C12 Myotubes via G Protein-Coupled Receptor 41

The Effects of Propionate and Valerate on Insulin Responsiveness for Glucose Uptake in 3T3-L1 Adipocytes and C2C12 Myotubes via G Protein-Coupled Receptor 41

Eriodictyol inhibits high glucose‐induced oxidative stress and inflammation in retinal ganglial cells

Natural Phyto-Bioactive Compounds for the Treatment of Type 2 Diabetes: Inflammation as a Target

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