Effect of equilibration periods on post-thaw semen quality of Jamunapari bucks

Ramachandran, N.; Yadav, Sushma; Sikarwar, A.K.S.; Saraswat, Sonia; Ranjan, Ravi; Jindal, Shalini

doi:10.5958/0973-9718.2015.00053.7

Cited by 7 publications

(7 citation statements)

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“…In addition, the cryopreservation process causes nuclear damage and DNA disintegration due to the production of reactive oxygen species (ROS), which ultimately leads to lipid peroxidation, membrane damage and death of the spermatozoa (Zamiri, 2020). Mean value of post thaw progressive sperm motility (29.75±1.87) in the present study was higher than the findings of Ramachandran et al (2015) in Jamunapari bucks (28.82±1.99) and Tabarez et al (2017) in Blanca de Rasquera bucks (18.2-24.5) and lesser than Black Bengal bucks (40.89±0.65) (Singh et al, 2016), Alpine Sannen and Beetal crosses (45-56) (Narwade et al, 2017).…”

Section: Resultscontrasting

confidence: 91%

“…The mean values of per cent HOST-reactive spermatozoa (39.21±2.00) in present study were higher than Boer bucks (8.04±1.02) (Yodmingkwan et al, 2016), however, lower than Jamunapari bucks (41.01±3.02) (Ramachandran et al, 2015); Black Bengal bucks (47.02±0.58) (Singh et al, 2016); Gaddi bucks (52.48±1.43) (Sharma, 2018) and Alpine Sannen and Beetal crosses (61.0-64.63) (Narwade et al, 2017).…”

Section: Resultscontrasting

confidence: 69%

“…Mean percentage of sperm viability (38.64±1.93) in the present study was higher than as observed in Boer bucks (27.33±0.85) (Yodmingkwan et al, 2016) and lower than Jamunapari bucks (41.01±3.02) (Ramachandran et al, 2015), Gaddi bucks (45.26±1.32) (Sharma, 2018) and Black Bengal bucks (50.48±0.65) (Singh et al, 2016).…”

Section: Resultscontrasting

confidence: 54%

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In vivo Fertility Assessment of Frozen Thawed Pantja Buck Semen

Dhara,

Gupta,

Thakur

et al. 2023

AJDFR

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Background: Pantja is a medium sized, dual purpose Tarai goat breed of Uttarakhand and adjacent area of Uttar Pradesh. Due to the lack of pure breeding buck and uncontrolled cross breeding, population of this breed is reducing continuously. Therefore, for preservation and dissemination of this pure breed, cryopreservation of semen and artificial insemination by using frozen thawed semen can play a crucial role. Methods: Semen samples were collected from four sexually matured Pantja bucks and after preliminary evaluation extended in tris egg yolk based extender. Post thaw semen was examined for microscopical and certain biochemical evaluation. A total 12 does were inseminated and pregnancy diagnosis was conducted at 45 days onwards by ultrasonography. Result: Post thaw seminal attributes were significantly (P£0.01) reduced in comparision to freshly diluted semen. Total six does (50.00%) were pregnant and five (83.33%) of them gave birth to healthy kids. Among these five, three does (60.00%) gave birth to single and two does (40.00%) gave birth to twins.

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Section: Resultscontrasting

confidence: 91%

Section: Resultscontrasting

confidence: 69%

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In vivo Fertility Assessment of Frozen Thawed Pantja Buck Semen

Dhara,

Gupta,

Thakur

et al. 2023

AJDFR

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“…The lower (Srinivas et al, 2002;Sundararaman & Edwin, 2003;Naing et al, 2011) and higher seminal parameters under intensive (Kulaksiz & Daskin, 2010;Salviano et al, 2012;Fonseca et al, 2013;Qureshi et al, 2013;Ahmad et al, 2014) and semi-intensive (Thakur et al, 2005;Hassan et al, 2010) rearing systems have also been reported. The variation in semen quality parameters under different rearing systems in the present study could be due to variation in nutrient availability for semen production, breed and age of bucks which corroborates earlier findings (Mekasha et al, 2007;Bucak & Uysal, 2008;Mohamed & Abdelatif, 2010;Naing et al, 2011;Mellado et al, 2012;Ramachandran et al, 2015).…”

Section: Impact On Seminal Traitssupporting

confidence: 81%

Effect of management system on nutrient intake and seminal attributes in jamunapari bucks

Ramachandran¹,

Singh²,

Shinde³

et al. 2016

JEBAS

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The present study assessed comparative semen production potential of Jamunapari bucks under stall feeding (SF) and grazing cum supplementation (GS) systems of 10 animals in each system under group feeding and management conditions. SF Bucks received 500 g concentrate pellets and 700 g green fodder/h/d besides ad lib dry fodder for continuous one year. GS Bucks were allowed 4-6 hr daily grazing and supplemented concentrate pellets @ 500 g/h/d. Intake of nutrients by bucks in two groups were calculated, semen were collected using AV method biweekly to assess seminal traits. The apparent digestibility coefficients of dry matter, crude protein and hemi cellulose were higher in feed materials consumed by GS bucks than SF bucks. However, neutral detergent fibre, acid detergent fibre and cellulose digestibility had shown a reverse trend. The overall Least Square Mean (LSM) for intake of DM, OM, CP, NDF, ADF, hemicellulose, cellulose, ME install-fed bucks were 1309. 38, 1190.62, 176.99, 728.72, 410.08, 306.11, 266.09 g/day, 7.76 MJ/d, respectively. The respective nutrients intake in GS bucks were 1396.14, 1259.67, 198.87, 685.24, 367.99, 335.99, 211.23 g/day, 8.21MJ/d. LSM for volume (ml), color (1-2 scale), consistency (1-4 scale), mass activity (0-5 scale), initial progressive motility (%), sperm density(10 6 /ml), live sperms (%) and abnormal sperms (%) of semen produced by SF bucks were 0.92, 1.19, 2.86, 4.30, 78.21, 4317.41, 82.96, and 1.77, respectively. The respective values for the semen of GS bucks were 0.69, 1.24, 2.67, 3.64, 72.78, 3183.14, 74.87 and 2.18.Analysis of data revealed that SF bucks performed better (P<0.01) with lower nutrients intake than the GS bucks.

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“…Traditionally, goat frozen semen straw is thawed in a water bath at 37°C for 12-30 s (Narwade et al, 2017;Sharma et al, 2018;Sharma and Sood, 2019). The combination of duration and temperature of thawing for 45 s at 40°C (Ramachandran et al, 2015), for 20 s at 40°C (Sharma et al, 2018) have been studied with various results. Thawing of frozen semen will reactivate spermatozoa physiologically; hence, thawing has to be done carefully at an optimal temperature with sufficient time to minimize the decrease in semen quality (Borah et al, 2015).…”

Section: The Thawing Temperaturesmentioning

confidence: 99%

Combination of nanoparticle green tea extract in tris-egg yolk extender and 39 °c thawing temperatures improve the sperm quality of post-thawed Kacang goat semen

et al. 2022

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Kacang goats are small ruminants produced by low-income households in smallholder and farm to reduce poverty and prevent undernutrition. Studies to find a cryopreservation protocol for Kacang goat semen are expected to multiplication of genetically superior animals selected by the paternal lineage. This study evaluated the effect of thawing temperature and supplementation of the green tea extract nanoparticle in skim milk-egg yolk (SM-EY) extender on post-thaw sperm quality of Kacang goat semen. Six ejaculates of Kacang goat were diluted in SM-EY supplemented or not (control group) with 0.001 mg/mL NPs GTE. The diluted semen was packaged with 0.25 mL straws (insemination dose: 60x10 6 sptz/mL) and cryopreserved. Then, six samples of the control group and NPs GTE groups were thawed at 37°C or 39°C sterile water for 30 s and submitted to sperm quality evaluations. The sperm viability, motility, and intact of the plasma membrane (IPM) were higher (p<0.05) in NPs GTE group than control group. In contrast, the NPs GTE group presented lower (p<0.05) malondialdehyde levels and sperm DNA fragmentation (SDF) compared with the control group. The catalase levels were not significantly different (p > 0.05) between the control and NPs GTE groups. Thawing at 39°C resulted in higher (p<0.05) sperm viability, motility, and IPM than thawing at 37°C. However, thawing at 39°C group presented lower (p<0.05) malondialdehyde levels compared with thawing at 37°C. SDF and catalase levels were similar (p>0.05) between thawing at 37°C and thawing at 37°C. In conclusion, supplementation of 0.001 mg/mL of NPs GTE in SM-EY extender and thawing temperature of 39°C resulted in a better quality of frozen-thawed Kacang goat semen.

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Effect of equilibration periods on post-thaw semen quality of Jamunapari bucks

Cited by 7 publications

References 0 publications

In vivo Fertility Assessment of Frozen Thawed Pantja Buck Semen

In vivo Fertility Assessment of Frozen Thawed Pantja Buck Semen

Effect of management system on nutrient intake and seminal attributes in jamunapari bucks

Combination of nanoparticle green tea extract in tris-egg yolk extender and 39 °c thawing temperatures improve the sperm quality of post-thawed Kacang goat semen

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